LOCUS       Exported                6930 bp ds-DNA     circular SYN 13-APR-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 11, 2020 from SnapGene Viewer 5.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     248..267
                     /label=BR-0457
                     /note="color: black; sequence: ccacgggtgcgcatgatcgt"
     primer_bind     1146..1170
                     /label=BR-0164
                     /note="color: black; sequence: ATCAGGCGCTCTTCCGCTTCCTCGC"
     primer_bind     complement(1146..1170)
                     /label=BR-0163
                     /note="color: black; sequence: GCGAGGAAGCGGAAGAGCGCCTGAT"
     primer_bind     complement(1157..1185)
                     /label=SR-0576
                     /note="color: black; sequence: 
                     gcagcgagtcagtgagcgaggaagcggaa"
     primer_bind     1161..1192
                     /label=SR-0575
                     /note="color: black; sequence: 
                     gcttcctcgctcactgactcgctgcgctcggt"
     primer_bind     complement(1220..1251)
                     /label=BR-0054
                     /note="color: black; sequence: 
                     CTGTGGATAACCGTATTACCGCCTTTGAGTGA"
     primer_bind     1220..1251
                     /label=BR-0053
                     /note="color: black; sequence: 
                     TCACTCAAAGGCGGTAATACGGTTATCCACAG"
     primer_bind     complement(1299..1318)
                     /label=KJ-0353
                     /note="color: black; sequence: TTTTTACGGTTCCTGGCCTT"
     rep_origin      complement(1319..2001)
                     /direction=LEFT
                     /label=ColE1 Origin
                     /note="color: #9eafd2"
     primer_bind     complement(1845..1871)
                     /label=JY-0271
                     /note="color: black; sequence: GCTACCAGCGGTGGTTTGTTTGCCGGA"
     primer_bind     complement(1845..1871)
                     /label=JY-0287
                     /note="color: black; sequence: GCTACCAGCGGTGGTTTGTTTGCCGGA"
     primer_bind     1846..1872
                     /label=BR-0017
                     /note="color: black; sequence: CCGGCAAACAAACCACCGCTGGTAGCG"
     primer_bind     complement(2018..2046)
                     /label=KJ-0217
                     /note="color: black; sequence: 
                     aacaccccttgtattactgtttatgtaaG"
     CDS             2047..2859
                     /label=KanR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     2047..2068
                     /label=JZ-0309
                     /note="color: black; sequence: atgagccatattcaacgggaaa"
     primer_bind     2412..2451
                     /label=HZ-73
                     /note="color: black; sequence: 
                     TGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCT"
     primer_bind     2412..2451
                     /label=HZ-073
                     /note="color: black; sequence: 
                     TGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCT"
     primer_bind     2419..2444
                     /label=BCD-0583
                     /note="color: black; sequence: gcagtgttcctgcgccggttgcattc"
     primer_bind     complement(2574..2599)
                     /label=HZ-33
                     /note="color: black; sequence: GTTTATGCATTTCTTTCCAGACTTGT"
     primer_bind     complement(2574..2599)
                     /label=HZ-033
                     /note="color: black; sequence: GTTTATGCATTTCTTTCCAGACTTGT"
     primer_bind     2860..2884
                     /label=KJ-0352
                     /note="color: black; sequence: taagaattaattcatgagcggatac"
     primer_bind     2863..2891
                     /label=KJ-0216
                     /note="color: black; sequence: 
                     gaattaattcatgagcggatacatatttg"
     primer_bind     2894..2929
                     /label=BR-0182
                     /note="color: black; sequence: 
                     TGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGC"
     primer_bind     complement(2901..2929)
                     /label=BR-0458
                     /note="color: black; sequence: 
                     gcgcggaacccctatttgtttatttttct"
     rep_origin      complement(2955..3410)
                     /direction=LEFT
                     /label=M13 Origin
                     /note="color: #9eafd2"
     rep_origin      complement(3087..3393)
                     /direction=LEFT
                     /label=F1 ori
                     /note="color: #9eafd2"
     primer_bind     3403..3421
                     /label=BR-0631
                     /note="color: black; sequence: gggcgcgtcccattcgcca"
     misc_feature    3490..3508
                     /label=T7term
                     /note="color: #85dae9"
     primer_bind     3490..3508
                     /label=BR-0811
                     /note="color: black; sequence: GCTAGTTATTGCTCAGCGG"
     primer_bind     3512..3541
                     /label=BR-0052
                     /note="color: black; sequence: 
                     CAGCAGCCAACTCAGCTTCCTTTCGGGCTT"
     primer_bind     complement(3512..3541)
                     /label=BR-0051
                     /note="color: black; sequence: 
                     AAGCCCGAAAGGAAGCTGAGTTGGCTGCTG"
     primer_bind     complement(3529..3557)
                     /label=BR-0866
                     /note="color: black; sequence: 
                     GATCCGGCTGCTAACAAAGCCCGAAAGGA"
     primer_bind     complement(3534..3572)
                     /label=SR-0186
                     /note="color: black; sequence: 
                     CGCAAGGTCCTTTAAGATCCGGCTGCTAACAAAGCCCGA"
     primer_bind     complement(3536..3557)
                     /label=BR-0468
                     /note="color: black; sequence: GATCCGGCTGCTAACAAAGCCC"
     primer_bind     complement(3538..3557)
                     /label=HZ-200
                     /note="color: black; sequence: GATCCGGCTGCTAACAAAGC"
     primer_bind     3548..3582
                     /label=SR-0185
                     /note="color: black; sequence: 
                     CAGCCGGATCTTAAAGGACCTTGCGAATACCCGCA"
     misc_feature    complement(3558..5243)
                     /label=HIV RT
                     /note="color: #b7e6d7; direction: LEFT"
     misc_feature    complement(3747..3749)
                     /label=D498
                     /note="color: #ff9ccd; direction: LEFT"
     primer_bind     complement(3844..3870)
                     /label=SR-0188
                     /note="color: black; sequence: TGTTACCAACCGTGGCCGCCAGAAGGT"
     primer_bind     3846..3886
                     /label=SR-0187
                     /note="color: black; sequence: 
                     CTTCTGGCGGCCACGGTTGGTAACATAACCTGCTTTACCCA"
     misc_feature    complement(3912..3914)
                     /label=D443
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    3981..3983
                     /label=Ptofixint225
                     /note="color: #b1ff67"
     misc_feature    complement(4548..4550)
                     /label=G231
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    complement(4551..4553)
                     /label=M230
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    complement(4554..4556)
                     /label=W229
                     /note="color: #84b0dc; direction: LEFT"
     primer_bind     4575..4600
                     /label=SR-0144
                     /note="color: black; sequence: CTGATGTTTTTTATCCGGTGTGGTCA"
     misc_feature    complement(4683..4694)
                     /label=YMDD
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    complement(4689..4691)
                     /label=M184
                     /note="color: #faac61; direction: LEFT"
     primer_bind     complement(4751..4783)
                     /label=HZ-59
                     /note="color: black; sequence: 
                     GGAAAGGTAGTCCGGCAATTTTTCAGAGCAGCA"
     primer_bind     complement(4751..4783)
                     /label=HZ-059
                     /note="color: black; sequence: 
                     GGAAAGGTAGTCCGGCAATTTTTCAGAGCAGCA"
     primer_bind     complement(4759..4787)
                     /label=HZ-011
                     /note="color: black; sequence: 
                     GGTTGGAAAGGTAGTCCGGCAATTTTTCA"
     primer_bind     complement(4759..4787)
                     /label=HZ-11
                     /note="color: black; sequence: 
                     GGTTGGAAAGGTAGTCCGGCAATTTTTCA"
     primer_bind     4766..4784
                     /label=HZ-168
                     /note="color: black; sequence: TTGCCGGACTACCTTTCCA"
     misc_feature    complement(4785..4787)
                     /label=G152
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(4788..4790)
                     /label=Q151
                     /note="color: #ffef86; direction: LEFT"
     primer_bind     complement(4938..4967)
                     /label=HZ-068
                     /note="color: black; sequence: 
                     CTGGGTATTCCGCATCCGGCAGGTCTGAAA"
     primer_bind     complement(4938..4967)
                     /label=HZ-68
                     /note="color: black; sequence: 
                     CTGGGTATTCCGCATCCGGCAGGTCTGAAA"
     primer_bind     complement(4966..4997)
                     /label=HZ-066
                     /note="color: black; sequence: 
                     AAACGCACCCAGGATTTTTGGGAAGTTCAGCT"
     misc_feature    complement(4968..4970)
                     /label=Q91
                     /note="color: #9eafd2; direction: LEFT"
     misc_feature    complement(4974..4976)
                     /label=E89
                     /note="color: #faac61; direction: LEFT"
     primer_bind     complement(4983..5006)
                     /label=HZ-057
                     /note="color: black; sequence: GAACTGAATAAACGCACCCAGGAT"
     primer_bind     complement(4983..5006)
                     /label=HZ-57
                     /note="color: black; sequence: GAACTGAATAAACGCACCCAGGAT"
     primer_bind     complement(4984..5000)
                     /label=HZ-167
                     /note="color: black; sequence: AATAAACGCACCCAGGA"
     primer_bind     complement(4987..5003)
                     /label=HZ-172
                     /note="color: black; sequence: CTGAATAAACGCACCCA"
     primer_bind     complement(4991..5018)
                     /label=HZ-014
                     /note="color: black; sequence: 
                     GTGGATTTTCGTGAACTGAATAAACGCA"
     primer_bind     complement(4991..5009)
                     /label=HZ-162
                     /note="color: black; sequence: CGTGAACTGAATAAACGCA"
     primer_bind     complement(4995..5015)
                     /label=HZ-164
                     /note="color: black; sequence: GATTTTCGTGAACTGAATAAA"
     misc_feature    complement(4998..5000)
                     /label=N81
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(5001..5003)
                     /label=L80
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(5004..5006)
                     /label=E79
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(5007..5009)
                     /label=R78
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(5010..5012)
                     /label=F77
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(5013..5015)
                     /label=D76
                     /note="color: #ffef86; direction: LEFT"
     primer_bind     complement(5014..5045)
                     /label=HZ-64
                     /note="color: black; sequence: 
                     AAGGATAGCACCAAATGGCGTAAACTGGTGGA"
     primer_bind     complement(5014..5045)
                     /label=HZ-064
                     /note="color: black; sequence: 
                     AAGGATAGCACCAAATGGCGTAAACTGGTGGA"
     misc_feature    complement(5016..5018)
                     /label=V75
                     /note="color: #84b0dc; direction: LEFT"
     misc_feature    complement(5019..5021)
                     /label=L74
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(5025..5027)
                     /label=R72
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(5046..5048)
                     /label=K65
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    complement(5052..5054)
                     /label=I63
                     /note="color: #ffef86; direction: LEFT"
     primer_bind     complement(5218..5256)
                     /label=SR-0178
                     /note="color: black; sequence: 
                     TGGTGGAAGCGGTATGCCGATTAGCCCGATTGAAACCGT"
     primer_bind     5231..5265
                     /label=SR-0177
                     /note="color: black; sequence: 
                     GGCTAATCGGCATACCGCTTCCACCATGATGATGA"
     primer_bind     5256..5297
                     /label=BR-0661
                     /note="color: black; sequence: 
                     ATGATGATGATGATGGTGCATATGTATATCTCCTTCTTAAAG"
     primer_bind     5256..5285
                     /label=BR-0707
                     /note="color: black; sequence: 
                     ATGATGATGATGATGGTGCATATGTATATC"
     misc_feature    complement(5256..5273)
                     /label=N-His Tag
                     /note="color: #c7b0e3; direction: LEFT"
     primer_bind     complement(5274..5328)
                     /label=BR-0348
                     /note="color: black; sequence: 
                     ACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATG"
     primer_bind     5274..5314
                     /label=BR-0166
                     /note="color: black; sequence: 
                     CATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTC"
     primer_bind     5277..5314
                     /label=KJ-0128
                     /note="color: black; sequence: 
                     ATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTC"
     primer_bind     5307..5346
                     /label=BR-0349
                     /note="color: black; sequence: 
                     ATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCC"
     misc_binding    complement(5323..5345)
                     /label=LacO
                     /note="color: #84b0dc; direction: LEFT"
     primer_bind     complement(5345..5364)
                     /label=T7
                     /note="color: #85dae9; direction: LEFT"
     primer_bind     complement(5388..5407)
                     /label=BR-0627
                     /note="color: black; sequence: cgtccggcgtagaggatcga"
     primer_bind     complement(5429..5447)
                     /label=BR-0177
                     /note="color: black; sequence: AGCAACCGCACCTGTGGCG"
     primer_bind     complement(5444..5464)
                     /label=DL-0096
                     /note="color: black; sequence: TCGGCGATATAGGCGCCAGCA"
     primer_bind     complement(5444..5464)
                     /label=BR-0177*
                     /note="color: black; sequence: TCGGCGATATAGGCGCCAGCA"
     CDS             5878..6834
                     /label=LacI
                     /note="color: #9eafd2; direction: RIGHT"
     primer_bind     complement(6422..6442)
                     /label=JZ-0345
                     /note="color: black; sequence: tggtttgttgaaaaccggaca"
     primer_bind     complement(6787..6827)
                     /label=KK-0037
                     /note="color: black; sequence: 
                     CTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGA"
ORIGIN
        1 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
       61 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
      121 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
      181 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
      241 ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg
      301 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg
      361 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc
      421 gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc
      481 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg
      541 accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca
      601 acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg
      661 tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac
      721 caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct
      781 tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca
      841 cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa
      901 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
      961 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     1021 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     1081 gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     1141 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1201 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1261 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1321 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1381 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1441 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1501 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1561 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1621 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1681 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1741 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     1801 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1861 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1921 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1981 gttaagggat tttggtcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2041 ggtgttatga gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac
     2101 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     2161 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     2221 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     2281 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     2341 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     2401 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     2461 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     2521 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2581 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2641 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2701 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2761 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2821 aataaattgc agtttcattt gatgctcgat gagtttttct aagaattaat tcatgagcgg
     2881 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2941 aaaagtgcca cctgaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg
     3001 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3061 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     3121 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     3181 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     3241 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     3301 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     3361 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     3421 aatccggata tagttcctcc tttcagcaaa aaacccctca agacccgttt agaggcccca
     3481 aggggttatg ctagttattg ctcagcggtg gcagcagcca actcagcttc ctttcgggct
     3541 ttgttagcag ccggatctta aaggaccttg cgaatacccg cagaaaccag tttgtcaact
     3601 tgctcgttcc ctccaatacc tttatgagcc gggacccaag ccagatacac cttctccttt
     3661 ttaatcagct gctcaataat ttgatttacc agttcacttt cagactgatc cggctgggcc
     3721 tgaataatcc caagtgcgta ctggctgtcc gtaacgatgt ttacttccag accgctatct
     3781 tgaagcgcca agtaaatcgc ttgtaattct gttttctgat tcgtggtgtc cgttaaagta
     3841 actaccttct ggcggccacg gttggtaaca taacctgctt tacccagttt ggtttcgcga
     3901 tttgctgcac catcaacata aaaggtttcg gcaccaacaa tcggttcttt ttctaactga
     3961 taccacagtt tcaccagcgg aggggtatta acaaattccc attcaggaat ccaggttgcc
     4021 tgccaatatt cggtccacca agtttcccat gtctctttct gaatcggcag tttgaatttc
     4081 ggggttttac cccaaataac aatgctttcg gttgtgattt tctgaacggc ttcagtcagc
     4141 tgtttcacat cattggtatg tgcaccacgc atacgtgcat atttaccggt tttcagattc
     4201 ttgaacggtt cttgataaat ctgataggtc cactgacctt gaccttgttt ctggatctcg
     4261 gcaatcagat ctttgctcgg atcataataa acgccgtgca caggttcttt cagaatttca
     4321 cgattttctg ccagttccag ttctgcttct tccgtcagcg gaataacttc tgtcagtgct
     4381 tttgtaccac gcagcagttt acacagctga cgaactttaa tacccggata gatctggctt
     4441 gcccaattca gtttgccaac cagtttctga atatcattaa cggtccagct atctttttcc
     4501 ggcagcacaa tcggctgaac ggtccattta tccggatgca gttcataacc catccacaga
     4561 aaaggcggtt ctttctgatg ttttttatcc ggtgtggtca gaccccaacg cagcagatgc
     4621 tgacgcagtt cttcaatttt tgtacgatgc tgaccaattt ccagatcgct accaacatac
     4681 agatcatcca tatactggta gatcacaata tccggattct gtttacgaaa cggttccaga
     4741 attttggtca tgctgctctg aaaaattgcc ggactacctt tccaaccttg cggcagaaca
     4801 ttatactgat agcgaatacc cggtgtttcg ttattaatgc tcgggatcgt aaatgcggta
     4861 tatttacgaa aatcttcatc cagcggaacg ctaaaatatg catcacccac atccagaact
     4921 gtaacgcttt tctttttttt cagacctgcc ggatgcggaa tacccagctg aacttcccaa
     4981 aaatcctggg tgcgtttatt cagttcacga aaatccacca gtttacgcca tttggtgcta
     5041 tcctttttct tgatggcaaa aaccggtgta ttatacggat tttccggacc aattttgctg
     5101 attttgcctt ctttttccat ctcggtgcaa atttccacca gtgctttgat tttttcttcg
     5161 gtcagaggcc actgtttaac tttcggacca tccataccag gtttcagttt aaccggaacg
     5221 gtttcaatcg ggctaatcgg cataccgctt ccaccatgat gatgatgatg gtgcatatgt
     5281 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca
     5341 attcccctat agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctctacg
     5401 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     5461 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     5521 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     5581 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     5641 tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
     5701 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
     5761 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
     5821 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
     5881 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
     5941 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
     6001 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
     6061 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
     6121 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
     6181 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
     6241 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
     6301 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
     6361 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
     6421 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
     6481 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
     6541 cgcgttggtg cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
     6601 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     6661 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
     6721 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6781 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     6841 caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt
     6901 gagagccttc aacccagtca gctccttccg
//
