LOCUS       Exported                7790 bp ds-DNA     circular SYN 22-APR-2020
DEFINITION  Tgatcc motif at MCS is a result of issues cloning in with BamHI. Had
            to use a compatable sticky end from BclI-HF79.9 kDa
            Isoelectric point (pI) : 7.77
            Charge at pH 7.0: 9.52.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Alex Brown
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 11, 2020 from SnapGene Viewer 5.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        139..167
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                      ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     gene            209..1069
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                      ALWVI 
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL 
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG 
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             209..1069
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    2242..2264
                     /label=pGEX_3_primer
                     /note="/translation=MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                      ALWVI 
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL 
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG 
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    2258..2449
                     /label=ROP
                     /note="/translation=MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                      ALWVI 
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL 
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG 
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             complement(3088..3705)
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIF
                     GEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLA
                     FATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLI
                     VTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            complement(3088..3702)
                     /gene="tet (576 - 1190)"
                     /label=tet (576 - 1190)
                     /note="/translation=MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                      ALWVI 
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL 
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG 
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             3283..3978
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQR
                     VGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPERCR
                     HLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVALTAR
                     FPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAASGPRW
                     FAPAGENPV"
     misc_feature    3761..4852
                     /label=lacI
                     /note="/translation=MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNL
                      LLGCF 
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD 
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV 
                     ANCRGD*"
     CDS             complement(3761..4720)
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVS
                     LVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"
     CDS             complement(4593..5093)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFL
                     MQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSDRF
                     PRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAVANC
                     RGD"
     misc_feature    5127..5146
                     /label=pBRrevBam_primer
     primer_bind     5127..5146
                     /label=pBRrevBam_primer.FOR
     promoter        5230..5248
                     /label=T7_promoter
     operon          5248..5275
                     /operon="Lac"
                     /label=operator
                     /label=lacO
     misc_feature    5293..5309
                     /label=T7_transl_en_RBS
     CDS             5317..5382
                     /codon_start=1
                     /label=Linker
                     /translation="MGHHHHHHHHHHSSGHIDDDDK"
     misc_feature    5323..5352
                     /label=10x His Tag
     misc_feature    5371..5382
                     /label=Enterokinase
                     /label=EK
     primer_bind     5383..5395
                     /label=MMLV-RT NdeI front.FOR
     misc_feature    5383..5388
                     /label=MCS
     CDS             5389..5409
                     /codon_start=1
                     /label=MMLV-RT upstream segment
                     /translation="GQPLQVL"
     misc_feature    5396..6095
                     /label=MMLV-RT NdeI front.FOR extension
     CDS             5410..7461
                     /codon_start=1
                     /label=MMLV RT mutant
                     /translation="TLNIEDEHRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGPPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPLPDA
                     DHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIALTQALKMAEGK
                     KLNVYTDSRYAFATAHIHGEIYRRRGWLTSKGKEIKNKDEILALLKALFLPKRLSIIHC
                     PGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSPNSRLIN"
     misc_feature    5610..5614
                     /label=CCTGG
     misc_feature    5658..5662
                     /label=CCTGG
     unsure          5824..5826
                     /label=L139P
     primer_bind     5875..5895
                     /label=MMLV-RT 1:2.FOR
     misc_feature    5896..6595
                     /label=MMLV-RT 1:2.FOR extension
     misc_feature    5943..5946
                     /label=GATC
     misc_feature    5960..5964
                     /label=CCTGG
     unsure          6007..6009
                     /label=D200N
     misc_feature    6025..6028
                     /label=GATC
     misc_feature    6055..6058
                     /label=GATC
     primer_bind     complement(6097..6120)
                     /label=MMLV-RT NdeI:1.REV
     misc_feature    6147..6151
                     /label=CCTGG
     misc_feature    6189..6192
                     /label=GATC
     misc_feature    6213..6217
                     /label=CCTGG
     misc_feature    6318..6322
                     /label=CCTGG
     misc_feature    6348..6351
                     /label=GATC
     primer_bind     6356..6374
                     /label=MMLV-RT 2:3.FOR
     misc_feature    6375..7074
                     /label=MMLV-RT 2:3.FOR extension
     unsure          6397..6399
                     /label=T330P
     misc_feature    6424..6427
                     /label=GATC
     misc_feature    6441..6445
                     /label=CCTGG
     misc_feature    6447..6450
                     /label=GATC
     primer_bind     complement(6596..6616)
                     /label=MMLV-RT 1:2.REV
     misc_feature    6708..6711
                     /label=GATC
     misc_feature    6711..6715
                     /label=CCTGG
     misc_feature    6735..6739
                     /label=CCTGG
     misc_feature    6789..6793
                     /label=CCTGG
     misc_feature    6811..6814
                     /label=GATC
     primer_bind     6849..6870
                     /label=MMLV-RT 3:XholI.FOR
     misc_feature    6871..7462
                     /label=MMLV-RT 3:XholI.FOR extension
     misc_feature    6961..6964
                     /label=GATC
     misc_feature    6968..6972
                     /label=CCTGG
     primer_bind     complement(7073..7091)
                     /label=MMLV-RT 2:3.REV
     misc_feature    7098..7101
                     /label=GATC
     unsure          7216..7218
                     /label=L603W
     unsure          7228..7230
                     /label=E607K
     misc_feature    7368..7372
                     /label=CCTGG
     primer_bind     complement(7448..7466)
                     /label=MMLV-RT XholI rear.REV
     misc_feature    7462..7472
                     /label=MCS
     misc_RNA        7462
                     /label=MMLV-RT 3:XholI.FOR extension
     terminator      7475..7603
                     /label=T7_terminator
     misc_feature    7517..7535
                     /label=T7_Terminal_primer
     primer_bind     complement(7517..7535)
                     /label=T7 Terminal Primer.REV
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
     3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
     3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
     4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
     4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
     4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
     4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
     4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
     4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
     4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
     4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
     4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
     4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
     4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
     4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
     4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
     4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
     4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
     5281 aaataatttt gtttaacttt aagaaggaga tataccatgg gccatcatca tcatcatcat
     5341 catcatcatc acagcagcgg ccatatcgac gacgacgaca agcatatggg ccagccgctc
     5401 caagtgctca ccctgaacat cgaagacgaa catcgcttgc acgaaaccag caaagaaccg
     5461 gatgtttcac tgggttccac ttggctgtcg gactttccgc aagcgtgggc agaaaccggc
     5521 ggtatgggtc tggctgtgcg ccaagctccg ctgattatcc cactgaaggc aactagtacc
     5581 ccggtatcca ttaaacagta cccgatgagc caggaggccc gtctgggcat taaaccgcat
     5641 atccagcgtc tgctggacca gggtatctta gtcccgtgcc agtccccgtg gaacaccccg
     5701 ctgctgccgg ttaaaaaacc gggcaccaac gactatcgcc cggtacaaga tttacgtgaa
     5761 gttaacaagc gcgtagaaga tatccatccg accgttccca acccgtacaa tctgctctca
     5821 ggtccgccgc cttcccacca atggtacacc gttctggacc tgaaagatgc cttcttttgc
     5881 ttgcgtctgc atccgactag ccagcccctg tttgcattcg agtggcgtga cccggaaatg
     5941 gggatcagcg gccagctcac ctggacccgt ctgccgcaag gtttcaaaaa ctcacctacc
     6001 ctgtttaatg aagctctgca ccgcgatctg gcggatttcc gcattcaaca cccggatctg
     6061 attctgctgc agtatgtgga cgacctgctg ctggcggcta cctccgaact ggattgccag
     6121 cagggcaccc gtgccttact gcaaaccctg ggtaacttag gttatcgtgc tagcgcgaaa
     6181 aaagcccaga tctgtcagaa gcaggttaaa tacctgggtt acctcctgaa agaaggccag
     6241 cgctggttaa ccgaagctcg caaggagacg gtcatgggtc agccaacccc gaaaaccccg
     6301 cgccagctgc gtgagttcct gggcaccgcg ggcttttgtc gcctgtggat cccgggcttc
     6361 gcggaaatgg ctgcgccgct gtacccgctc accaaaccgg ggaccctgtt caactggggt
     6421 ccggatcagc aaaaggctta ccaggagatc aaacaggcgt tgctgaccgc accagctctc
     6481 ggtctgccgg acctgacgaa accgtttgaa ctgtttgtgg acgaaaaaca gggttacgca
     6541 aaaggtgttc tgacccagaa actgggtcca tggcgtcgcc cggtcgccta cctgagcaag
     6601 aaactggacc cggttgcagc tggctggccg ccgtgtctcc gtatggtagc tgcaatcgct
     6661 gtgctgacca aagatgcggg aaaactgacc atgggtcaac cgttggtgat cctggcaccg
     6721 catgcggtag aagccctggt gaaacagccg ccggaccgtt ggctgtccaa cgcccgcatg
     6781 acgcactacc aggcgctgct gctggatacc gatcgtgttc agttcggccc ggttgttgcg
     6841 ctgaacccgg caactctgct tccgttaccg gaagagggtc tgcagcacaa ctgccttgat
     6901 atcctcgcag aagcgcacgg cacccgtcct gacctgacgg accagccact gccggacgca
     6961 gatcatacct ggtacaccga cggcagcagc ctgctgcaag agggccaacg caaagcgggg
     7021 gcagccgtaa ccactgagac cgaggttatt tgggctaaag ctctgccagc cggcacttct
     7081 gcacagcgtg cggaactgat cgctctgacc caagccctga aaatggctga aggcaagaaa
     7141 ttgaacgttt acaccgacag ccgctatgcg ttcgcgactg cccacatcca cggtgaaatc
     7201 taccgccgcc gcggttggct gacgtcgaag ggcaaagaaa tcaaaaacaa agatgaaatc
     7261 ttagctctgc tgaaagctct gtttctgccg aaacgtctgt ccattattca ctgcccgggt
     7321 caccagaaag ggcactctgc agaagctcgt ggaaaccgta tggcagacca ggctgcgcgt
     7381 aaagcagcca tcactgaaac cccggatacg tcaactctgc tgattgaaaa cagcagcccg
     7441 aacagccgct tgattaactg actcgatgat ccggctgcta acaaagcccg aaaggaagct
     7501 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     7561 gtcttgaggg gttttttgct gaaaggagga actatatccg gatatcccgc aagaggcccg
     7621 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga
     7681 cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg
     7741 ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
//
