LOCUS       Exported                9271 bp ds-DNA     circular SYN 26-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2020 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..635
                     /label=HIV-1 5'LTR
     primer_bind     2..24
                     /label=HIV-1 5' LTR
     misc_feature    685..822
                     /label=psi
     primer_bind     764..782
                     /label=G00
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                     /label=cPPT
     misc_feature    2185..2788
                     /label=pCMV
     primer_bind     2659..2679
                     /label=CMV_F
     primer_bind     2822..2868
                     /label=pLVX_Phi3_AURKB_CF_F
     misc_feature    2849..3883
                     /label=AURKB
     primer_bind     complement(3025..3044)
                     /label=AURKB_CP_R
     primer_bind     complement(3864..3904)
                     /label=pLVX_Phi3_AURKB_CF_R
     misc_feature    3936..4444
                     /label=pPGK
     primer_bind     complement(4376..4395)
                     /label=pGK_Rvs
     CDS             4468..5178
                     /label=mCherry
     primer_bind     complement(4613..4631)
                     /label=mCherry_R
     primer_bind     4881..4900
                     /label=mCherry_Fwd
     misc_feature    5193..5784
                     /label=WPRE
     primer_bind     complement(5245..5265)
                     /label=WPRE_R
     misc_feature    5988..6624
                     /label=HIV-1 3'UTR
     misc_feature    complement(7094..7764)
                     /label=pUC Ori
     misc_feature    complement(7909..8905)
                     /label=AmpR
     primer_bind     8533..8552
                     /label=AmpR_Fwd
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc tgaagcttag atctcgagaa
     2821 ttcgcggccg ctccggacgc gtgccaccat ggcccagaag gagaactcct acccctggcc
     2881 ctacggccga cagacggctc catctggcct gagcaccctg ccccagcgag tcctccggaa
     2941 agagcctgtc accccatctg cacttgtcct catgagccgc tccaatgtcc agcccacagc
     3001 tgcccctggc cagaaggtga tggagaatag cagtgggaca cccgacatct taacgcggca
     3061 cttcacaatt gatgactttg agattgggcg tcctctgggc aaaggcaagt ttggaaacgt
     3121 gtacttggct cgggagaaga aaagccattt catcgtggcg ctcaaggtcc tcttcaagtc
     3181 ccagatagag aaggagggcg tggagcatca gctgcgcaga gagatcgaaa tccaggccca
     3241 cctgcaccat cccaacatcc tgcgtctcta caactatttt tatgaccgga ggaggatcta
     3301 cttgattcta gagtatgccc cccgcgggga gctctacaag gagctgcaga agagctgcac
     3361 atttgacgag cagcgaacag ccacgatcat ggaggagttg gcagatgctc taatgtactg
     3421 ccatgggaag aaggtgattc acagagacat aaagccagaa aatctgctct tagggctcaa
     3481 gggagagctg aagattgctg acttcggctg gtctgtgcat gcgccctccc tgaggaggaa
     3541 gacaatgtgt ggcaccctgg actacctgcc cccagagatg attgaggggc gcatgcacaa
     3601 tgagaaggtg gatctgtggt gcattggagt gctttgctat gagctgctgg tggggaaccc
     3661 accctttgag agtgcatcac acaacgagac ctatcgccgc atcgtcaagg tggacctaaa
     3721 gttccccgct tccgtgccca tgggagccca ggacctcatc tccaaactgc tcaggcataa
     3781 cccctcggaa cggctgcccc tggcccaggt ctcagcccac ccttgggtcc gggccaactc
     3841 tcggagggtg ctgcctccct ctgcccttca atctgtcgcc tgaggatccg gcgcgcccgg
     3901 gttcgaatgt acacgtacgt taattaatct agataattct accgggtagg ggaggcgctt
     3961 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     4021 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     4081 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     4141 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     4201 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     4261 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     4321 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     4381 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     4441 tcgacctgcc tcgaggcccg ggccaccatg gtgagcaagg gcgaggagga taacatggcc
     4501 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     4561 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     4621 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     4681 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     4741 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     4801 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     4861 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4921 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     4981 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     5041 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     5101 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     5161 gacgagctgt acaagtaacc gggtctggaa caatcaacct ctggattaca aaatttgtga
     5221 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5281 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5341 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5401 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5461 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5521 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5581 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5641 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5701 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5761 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     5821 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     5881 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     5941 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     6001 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     6061 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     6121 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     6181 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     6241 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     6301 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     6361 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     6421 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6481 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6541 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6601 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     6661 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     6721 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6781 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6961 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7561 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7801 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7861 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7921 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7981 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8041 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8101 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8161 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8221 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8281 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8341 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8401 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8461 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8521 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8581 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8641 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8701 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8761 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8821 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8881 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
     8941 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9001 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     9061 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     9121 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     9181 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta
     9241 tttgttaaat atgtactaca aacttagtag t
//
