LOCUS       Exported                9073 bp ds-DNA     circular SYN 22-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2020 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..635
                     /label=HIV-1 5'LTR
     misc_feature    685..822
                     /label=psi
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                     /label=cPPT
     misc_feature    2185..2788
                     /label=pCMV
     primer_bind     2822..2868
                     /label=pLVX_Phi3_ZSGreen_CF_F
     CDS             2849..3544
                     /label=ZsGreen1
     primer_bind     complement(3525..3561)
                     /label=pLVX_Phi3_ZSGreen_CF_R
     misc_feature    3559..3729
                     /label=Anti-miR-211-5p
     misc_feature    3738..4246
                     /label=pPGK
     CDS             4270..4980
                     /label=mCherry
     primer_bind     complement(4415..4433)
                     /label=mCherry_R
     primer_bind     4683..4702
                     /label=mCherry_Fwd
     misc_feature    4995..5586
                     /label=WPRE
     primer_bind     complement(5047..5067)
                     /label=WPRE_R
     misc_feature    5790..6426
                     /label=HIV-1 3'UTR
     misc_feature    complement(6896..7566)
                     /label=pUC Ori
     misc_feature    complement(7711..8707)
                     /label=AmpR
     primer_bind     8335..8354
                     /label=AmpR_Fwd
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc tgaagcttag atctcgagaa
     2821 ttcgcggccg ctccggacgc gtgccaccat ggcccagtcc aagcacggcc tgaccaagga
     2881 gatgaccatg aagtaccgca tggagggctg cgtggacggc cacaagttcg tgatcaccgg
     2941 cgagggcatc ggctacccct tcaagggcaa gcaggccatc aacctgtgcg tggtggaggg
     3001 cggccccttg cccttcgccg aggacatctt gtccgccgcc ttcatgtacg gcaaccgcgt
     3061 gttcaccgag tacccccagg acatcgtcga ctacttcaag aactcctgcc ccgccggcta
     3121 cacctgggac cgctccttcc tgttcgagga cggcgccgtg tgcatctgca acgccgacat
     3181 caccgtgagc gtggaggaga actgcatgta ccacgagtcc aagttctacg gcgtgaactt
     3241 ccccgccgac ggccccgtga tgaagaagat gaccgacaac tgggagccct cctgcgagaa
     3301 gatcatcccc gtgcccaagc agggcatctt gaagggcgac gtgagcatgt acctgctgct
     3361 gaaggacggt ggccgcttgc gctgccagtt cgacaccgtg tacaaggcca agtccgtgcc
     3421 ccgcaagatg cccgactggc acttcatcca gcacaagctg acccgcgagg accgcagcga
     3481 cgccaagaac cagaagtggc acctgaccga gcacgccatc gcctccggct ccgccttgcc
     3541 ctaaggatcc ggcgcgccag gcgaaggtca caaagggaat taaaggcgaa ggtcacaaag
     3601 ggaattaaag gcgaaggtca caaagggaat taaaggcgaa ggtcacaaag ggaattaaag
     3661 gcgaaggtca caaagggaat taaaggcgaa ggtcacaaag ggaattaaag gcgaaggtca
     3721 caaagggaat ctagataatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     3781 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     3841 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     3901 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     3961 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     4021 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     4081 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     4141 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     4201 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cctcgaggcc
     4261 cgggccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     4321 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     4381 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     4441 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     4501 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     4561 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     4621 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     4681 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     4741 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     4801 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     4861 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     4921 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     4981 ccgggtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5041 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5101 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5161 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5221 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5281 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5341 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     5401 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5461 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5521 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5581 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     5641 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     5701 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5761 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
     5821 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     5881 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     5941 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     6001 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     6061 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     6121 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     6181 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     6241 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6301 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6361 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6421 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6481 agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg
     6541 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6601 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6661 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6721 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6781 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6841 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6901 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6961 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7021 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7081 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7141 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7201 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7261 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7321 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7381 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7441 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7501 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7561 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7621 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7681 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7741 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7801 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7861 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7921 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7981 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8041 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8101 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8161 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8221 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8281 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8341 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8401 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8461 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8521 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8581 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8641 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8701 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
     8761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8821 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     8881 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     8941 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     9001 taaatatgta ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta
     9061 caaacttagt agt
//
