LOCUS       Exported                8926 bp ds-DNA     circular SYN 22-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2020 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..635
                     /label=HIV-1 5'LTR
     primer_bind     2..24
                     /label=HIV-1 5' LTR
     misc_feature    685..822
                     /label=psi
     primer_bind     764..782
                     /label=G00
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                     /label=cPPT
     misc_feature    2185..2788
                     /label=pCMV
     primer_bind     2659..2679
                     /label=CMV_F
     CDS             2843..3538
                     /label=ZsGreen1
     misc_feature    3591..4099
                     /label=pPGK
     CDS             4123..4833
                     /label=mCherry
     primer_bind     complement(4268..4286)
                     /label=mCherry_R
     primer_bind     4536..4555
                     /label=mCherry_Fwd
     misc_feature    4848..5439
                     /label=WPRE
     primer_bind     complement(4900..4920)
                     /label=WPRE_R
     misc_feature    5643..6279
                     /label=HIV-1 3'UTR
     misc_feature    complement(6749..7419)
                     /label=pUC Ori
     misc_feature    complement(7564..8560)
                     /label=AmpR
     primer_bind     8188..8207
                     /label=AmpR_Fwd
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc tgaagcttag atctcgagaa
     2821 ttcgcggccg ctccggacgc gtatggccca gtccaagcac ggcctgacca aggagatgac
     2881 catgaagtac cgcatggagg gctgcgtgga cggccacaag ttcgtgatca ccggcgaggg
     2941 catcggctac cccttcaagg gcaagcaggc catcaacctg tgcgtggtgg agggcggccc
     3001 cttgcccttc gccgaggaca tcttgtccgc cgccttcatg tacggcaacc gcgtgttcac
     3061 cgagtacccc caggacatcg tcgactactt caagaactcc tgccccgccg gctacacctg
     3121 ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc tgcaacgccg acatcaccgt
     3181 gagcgtggag gagaactgca tgtaccacga gtccaagttc tacggcgtga acttccccgc
     3241 cgacggcccc gtgatgaaga agatgaccga caactgggag ccctcctgcg agaagatcat
     3301 ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc atgtacctgc tgctgaagga
     3361 cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag gccaagtccg tgccccgcaa
     3421 gatgcccgac tggcacttca tccagcacaa gctgacccgc gaggaccgca gcgacgccaa
     3481 gaaccagaag tggcacctga ccgagcacgc catcgcctcc ggctccgcct tgccctaagg
     3541 atccggcgcg cccgggttcg aatgtacacg tacgttaatt aatctagata attctaccgg
     3601 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     3661 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     3721 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     3781 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     3841 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     3901 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     3961 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     4021 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     4081 tcatctccgg gcctttcgac ctgcctcgag gcccgggcca ccatggtgag caagggcgag
     4141 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     4201 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     4261 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     4321 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     4381 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     4441 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     4501 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     4561 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     4621 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     4681 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     4741 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     4801 tccaccggcg gcatggacga gctgtacaag taaccgggtc tggaacaatc aacctctgga
     4861 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     4921 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4981 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5041 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5101 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5161 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5221 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     5281 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5341 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5401 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     5461 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     5521 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     5581 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     5641 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     5701 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     5761 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     5821 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     5881 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     5941 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     6001 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     6061 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     6121 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6181 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6241 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6301 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     6361 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6421 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6481 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6541 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6601 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6661 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7201 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7261 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7861 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7921 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8161 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8401 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8461 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8521 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
     8581 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8641 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8701 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     8761 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     8821 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     8881 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
