LOCUS       Exported                3892 bp ds-DNA    circular SYN 12-DEC-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLXMA level 2A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   SynthSys Center
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 30, 2020 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      complement(2..42)
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    52..75
                     /note="assembly linker"
                     /note="color: #a6acb3"
     gene            complement(76..981)
                     /gene="aadA"
                     /note="aadA"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(76..981)
                     /codon_start=1
                     /gene="aadA"
                     /product="spectinomycin adenyltransferase"
                     /note="aadA"
                     /note="AadA"
                     /note="color: #993366"
                     /protein_id="ASG92126.1"
                     /translation="MPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIA
                     EVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRAL
                     INDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPA
                     TIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDER
                     NVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRA
                     DQLEEFVHYVKGEITKVVGK"
     CDS             complement(76..867)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /note="color: #ccffcc"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    1065..1088
                     /note="assembly linker"
                     /note="color: #a6acb3"
     gene            complement(1089..1751)
                     /gene="rep"
                     /note="rep"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(1089..1751)
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /note="rep"
                     /note="color: #993366"
                     /protein_id="ASG92127.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      1752..2523
                     /note="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
                     /note="color: #ffff00"
     misc_feature    2611..2634
                     /note="assembly linker"
                     /note="color: #a6acb3"
     regulatory      2635..2682
                     /note="color: #ffffff"
     misc_feature    2743..2767
                     /note="LB"
                     /note="color: #a6acb3"
     misc_feature    2790..2820
                     /note="LB"
                     /note="color: #a6acb3"
     misc_feature    2794..2818
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     misc_feature    2833..2838
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
                     /note="color: #99ccff"
     misc_feature    2846..2849
                     /note="GGAG Overlap"
                     /note="color: #ff0000"
     promoter        2861..2895
                     /note="J23106 promoter"
                     /note="color: #00ffff"
     RBS             2904..2915
                     /note="B0034 RBS"
                     /note="color: #ffff00"
     CDS             2922..3641
                     /codon_start=1
                     /note="sfGFP"
                     /note="color: #ffff00"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     YYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK*"
     terminator      3646..3717
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     terminator      3733..3760
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
                     /note="color: #ffffff"
     misc_feature    3772..3775
                     /note="ACCC Overlap"
                     /note="color: #ff0000"
     misc_feature    3782..3892
                     /note="RB from pTi6"
                     /note="color: #a6acb3"
     misc_feature    3806..3829
                     /note="RB"
                     /note="color: #a6acb3"
     misc_feature    3839..3867
                     /note="Overdrive"
                     /note="color: #a6acb3"
ORIGIN
        1 acaagaaaag aaaaaacacc cgttagggtg tttttagtta gtgtggcgcg ccgacttgcg
       61 acatgcggtc ctttgttatt tgccgactac cttggtgatc tcgcctttca cgtagtggac
      121 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
      181 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
      241 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
      301 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
      361 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
      421 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
      481 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
      541 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
      601 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
      661 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
      721 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
      781 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
      841 tacttcggcg atcaccgctt ccctcatgat gtttaacttt gttttagggc gactgccctg
      901 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg
      961 ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa
     1021 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttcgatcgg attggcggtt
     1081 atgcggttct accggcgcgg cagcgttacc cgtgtcggcg gctccaacgg ctcgccatcg
     1141 tccagaaaac acggctcatc gggcatcggc aggcgctgct gcccgcgccg ttcccattcc
     1201 tccgtttcgg tcaaggctgg caggtctggt tccatgcccg gaatgccggg ctggctgggc
     1261 ggctcctcgc cggggccggt cggtagttgc tgctcgcccg gatacagggt cgggatgcgg
     1321 cgcaggtcgc catgccccaa cagcgattcg tcctggtcgt cgtgatcaac caccacggcg
     1381 gcactgaaca ccgacaggcg caactggtcg cggggctggc cccacgccac gcggtcattg
     1441 accacgtagg ccgacacggt gccggggccg ttgagcttca cgacggagat ccagcgctcg
     1501 gccaccaagt ccttgactgc gtattggacc gtccgcaaag aacgtccgat gagcttggaa
     1561 agtgtcttct ggctgaccac cacggcgttc tggtggccca tctgcgccac gaggtgatgc
     1621 agcagcattg ccgccgtggg tttcctcgca ataagcccgg cccacgcctc atgcgctttg
     1681 cgttccgttt gcacccagtg accgggcttg ttcttggctt gaatgccgat ttctctggac
     1741 tgcgtggcca tgcttatctc catgcggtag gggtgccgca cggttgcggc accatgcgca
     1801 atcagctgca acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa gtggcagtca
     1861 attacagatt ttctttaacc tacgcaatga gctattgcgg ggggtgccgc aatgagctgt
     1921 tgcgtacccc ccttttttaa gttgttgatt tttaagtctt tcgcatttcg ccctatatct
     1981 agttctttgg tgcccaaaga agggcacccc tgcggggttc ccccacgcct tcggcgcggc
     2041 tccccctccg gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc ggccttcggc
     2101 cttgcccaag gtggcgctgc ccccttggaa cccccgcact cgccgccgtg aggctcgggg
     2161 ggcaggcggg cgggcttcgc ccttcgactg cccccactcg cataggcttg ggtcgttcca
     2221 ggcgcgtcaa ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt
     2281 ggtgtccaac cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa
     2341 attaaaaaaa ttgatggggc aaggccgcag gccgcgcagt tggagccggt gggtatgtgg
     2401 tcgaaggctg ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct
     2461 gtccatcagc ttgtccagca gggttgtcca cgggccgagc gaagcgagcc agccggtggc
     2521 cgctcgcggc catcgtccac atatccacgg gctggcaagg gagcgcagcg accgcgcagg
     2581 gcgaagcccg gagagcaagc ccgtaggggg ccatcatcag ttcggtggtc ttccgacgaa
     2641 caataaggcc gcaaatcgcg gcctttttta ttgataacaa aaccggctca gttctgcgta
     2701 gaaaccaaca tgcaagctcc accgggtgca aagcggcagc ggcggcagga tatattcaat
     2761 tgtaaatggc ttcatgtccg ggaaatctac tggtggcagg atatattgtg gtgtaaacaa
     2821 tggagaaaaa gagaattcgg tctcaggaga tatgcaggtg tttacggcta gctcagtcct
     2881 aggtatagtg ctagctacta gagaaagagg agaaatacta aatgcgtaaa ggcgaagaac
     2941 tgttcacggg cgtagttccg attctggtcg agctggacgg cgatgtgaac ggtcataagt
     3001 ttagcgttcg cggtgaaggt gagggcgacg cgaccaacgg caaactgacc ctgaagttca
     3061 tctgcaccac cggtaaactg ccggtgcctt ggccgacctt ggtgacgacg ttgacgtatg
     3121 gcgtgcagtg ttttgcgcgt tatccggacc acatgaaaca acacgatttc ttcaaatctg
     3181 cgatgccgga gggttacgtc caggagcgta ccatttcctt caaggatgat ggctactaca
     3241 aaactcgcgc agaggttaag tttgaaggtg acacgctggt caatcgtatc gaattgaagg
     3301 gtatcgactt taaagaggat ggtaacattc tgggccataa actggagtat aacttcaaca
     3361 gccataatgt ttacattacg gcagacaagc aaaagaacgg catcaaggcc aatttcaaga
     3421 ttcgccacaa tgttgaggac ggtagcgtcc aactggccga ccattaccag cagaacaccc
     3481 caattggtga cggtccggtt ttgctgccgg ataatcacta tctgagcacc caaagcgtgc
     3541 tgagcaaaga tccgaacgaa aaacgtgatc acatggtcct gctggaattt gtgaccgctg
     3601 cgggcatcac ccacggtatg gacgagctgt ataagtaatg agcttcaaat aaaacgaaag
     3661 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     3721 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg cacctgcata taccctgcag
     3781 gaattgttga ttttgtgatg actgatggca ggatatatgc ggttgtaatt catttttatt
     3841 gtctaaattt ctgtatttgt ttgtttgttc ggttgtaaat ttttttggaa ga
//
