LOCUS       Exported                3710 bp ds-DNA    circular SYN 17-JAN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    lvl0 fluc CDS
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   militzis
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 9, 2020 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      2..59
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
                     /note="color: #ffffff"
     rep_origin      complement(254..842)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1025..1119
                     /gene="
                     "
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
                     /note="color: #ffffff"
     CDS             complement(1140..1799)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1983..2026
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
                     /note="color: #ffffff"
     misc_feature    2029..2034
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
                     /note="This feature has 2 segments:
                        1: 2029 .. 2029 / #99ccff
                        2: 2030 .. 2034 / #99ccff"
     misc_feature    2042..2045
                     /note="AATG Overhang"
                     /note="color: #ff0000"
     CDS             2043..3695
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /EC_number="
                     "
                     /note="luciferase"
                     /note="enhanced luc+ version of the luciferase gene"
                     /note="color: #ffcc00"
                     /protein_id="
                     "
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    3695..3698
                     /note="GCTT Overlap"
                     /note="color: #ff0000"
     misc_feature    3699..3702
                     /note="TGAG Overlap"
                     /note="color: #ff0000"
     misc_feature    3706..1
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
                     /note="color: #a6ccff"
ORIGIN
        1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
       61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      241 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1021 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1081 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1141 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1201 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1261 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1321 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     1381 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1441 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1501 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1561 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1621 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1681 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1741 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     1921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     1981 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcggtctc
     2041 taatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
     2101 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
     2161 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
     2221 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
     2281 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
     2341 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
     2401 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
     2461 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
     2521 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
     2581 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
     2641 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
     2701 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     2761 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     2821 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
     2881 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
     2941 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     3001 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     3061 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     3121 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     3181 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     3241 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     3301 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     3361 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     3421 aacaccccaa catcttcgac gctggtgtcg caggtcttcc cgacgatgac gccggtgaac
     3481 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     3541 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     3601 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     3661 taaaggccaa gaagggcgga aagatcgccg tgtagctttg agaccctgca
//
