LOCUS       Exported                3510 bp ds-DNA    circular SYN 16-DEC-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    lvl0 sXVE
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   militzis
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 9, 2020 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      2..59
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
                     /note="color: #ffffff"
     rep_origin      complement(254..842)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1025..1119
                     /gene="
                     "
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
                     /note="color: #ffffff"
     CDS             complement(1140..1799)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1983..2026
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
                     /note="color: #ffffff"
     misc_feature    2029..2034
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
                     /note="This feature has 2 segments:
                        1: 2029 .. 2029 / #99ccff
                        2: 2030 .. 2034 / #99ccff"
     misc_feature    2042..2045
                     /note="AATG Overhang"
                     /note="color: #ff0000"
     misc_feature    2043..3494
                     /note="sXVE"
                     /note="color: #ff0000"
     misc_feature    3495..3498
                     /note="GCTT Overlap"
                     /note="color: #ff0000"
     misc_feature    3499..3502
                     /note="TGAG Overlap"
                     /note="color: #ff0000"
     misc_feature    3506..1
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
                     /note="color: #a6ccff"
ORIGIN
        1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
       61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      241 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1021 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1081 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1141 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1201 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1261 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1321 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     1381 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1441 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1501 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1561 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1621 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1681 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1741 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     1921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     1981 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcggtctc
     2041 taatgaaggc tcttactgct agacaacaag aagtttttga tcttattaga gatcatattt
     2101 ctcaaactgg aatgcctcct actagagctg aaattgctca aagacttgga tttagatctc
     2161 ctaatgctgc tgaagaacat cttaaggctc ttgctagaaa gggagttatt gaaattgttt
     2221 ctggagcttc aagaggaatt agacttcttc aagaagaaga agaaggactt cctcttgttg
     2281 gaagagttgc tgctggagaa ccttcttctg ctcctcctac tgatgtttct cttggagatg
     2341 aacttcatct tgatggagaa gatgttgcta tggctcatgc tgatgctctt gatgattttg
     2401 atcttgatat gcttggagat ggagattctc ctggacctgg atttactcct catgattctg
     2461 ctccttatgg agcacttgat atggctgatt ttgaatttga acaaatgttt actgatgctc
     2521 ttggaattga tgaatatgga ggagatcctt ctgctggaga tatgagagct gctaatcttt
     2581 ggccttctcc tcttatgatt aagaggtcta agaagaactc tcttgctctt tctcttactg
     2641 ctgaccaaat ggtttctgct cttcttgatg ctgaacctcc tattctttat tctgaatatg
     2701 atcctactag acctttttct gaggcttcta tgatgggact tcttactaat cttgctgata
     2761 gagaacttgt tcatatgatt aattgggcta agagagttcc tggatttgtt gatcttactc
     2821 ttcatgacca agttcatctt cttgaatgtg cttggcttga aattcttatg attggacttg
     2881 tttggaggtc tatggaacat cctgttaaac ttctttttgc tcctaatctt cttcttgata
     2941 gaaatcaagg aaagtgtgtt gaaggaatgg ttgaaatttt tgatatgctt cttgctactt
     3001 cttcaagatt tagaatgatg aatcttcaag gagaagaatt tgtttgtctt aagtctatta
     3061 ttcttcttaa ttctggagtt tatacttttc tttcttctac tcttaagtct cttgaagaaa
     3121 aggatcatat tcatagagtt cttgataaga ttactgatac tcttattcat cttatggcta
     3181 aggctggact tactcttcaa caacaacatc aaagacttgc tcaacttctt cttattcttt
     3241 ctcatattag acatatgtct aataagggaa tggaacatct ttattctatg aagtgtaaga
     3301 atgttgttcc tctttatgat cttcttcttg aaatgcttga tgctcataga cttcatgctc
     3361 ctacttcaag aggaggagct tctgttgaag aaactgacca atctcatctt gctactgctg
     3421 gatctacttc ttctcattct cttcaaaagt attatattac tggagaagct gaaggatttc
     3481 ctgctactgt ttgagctttg agaccctgca
//
