LOCUS       Exported                4284 bp ds-DNA    circular SYN 17-JAN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    lvl0 LhGR
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   militzis
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 9, 2020 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      2..59
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
                     /note="color: #ffffff"
     rep_origin      complement(254..842)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1025..1119
                     /gene="
                     "
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
                     /note="color: #ffffff"
     CDS             complement(1140..1799)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1983..2026
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
                     /note="color: #ffffff"
     misc_feature    2029..2034
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
                     /note="This feature has 2 segments:
                        1: 2029 .. 2029 / #99ccff
                        2: 2030 .. 2034 / #99ccff"
     misc_feature    2042..2045
                     /note="AATG Overhang"
                     /note="color: #ff0000"
     CDS             2043..4268
                     /codon_start=1
                     /note="LhGR"
                     /note="color: #ff0000"
                     /translation="MASEARKTKKKIKGIQQATAGVSQDTSENPNKTIVPAALPQLTPT
                     LVSLLEVIEPEVLYAGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLD
                     DQMTLLQYSWMFLMAFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFV
                     SSELQRLQVSYEEYLCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNS
                     SQNWQRFYQLTKLLDSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNG
                     NIKKLLFHQKSTSKPVTLYDVAEYAGVSHQTVSRVVNQASHVSAKTREKVEAAMAELNY
                     IPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEA
                     CKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHED
                     GTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMS
                     GFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYI
                     PPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTSGSEFANFNQSGN
                     IADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSK
                     PLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE"
     CDS             3924..4268
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /note="GAL4 activation domain"
                     /note="color: #993366"
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     misc_feature    4269..4272
                     /note="GCTT Overlap"
                     /note="color: #ff0000"
     misc_feature    4273..4276
                     /note="TGAG Overlap"
                     /note="color: #ff0000"
     misc_feature    4280..1
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
                     /note="color: #a6ccff"
ORIGIN
        1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
       61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      241 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1021 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1081 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1141 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1201 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1261 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1321 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     1381 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1441 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1501 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1561 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1621 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1681 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1741 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     1921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     1981 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcggtctc
     2041 taatggctag tgaagctcga aaaacaaaga aaaaaatcaa agggattcag caagccactg
     2101 caggagtctc acaagacact tcggaaaatc ctaacaaaac aatagttcct gcagcattac
     2161 cacagctcac ccctaccttg gtgtcactgc tggaggtgat tgaacccgag gtgttgtatg
     2221 caggatatga tagctctgtt ccagattcag catggagaat tatgaccaca ctcaacatgt
     2281 taggtgggcg tcaagtgatt gcagcagtga aatgggcaaa ggcgatacca ggcttcagaa
     2341 acttacacct ggatgaccaa atgaccctgc tacagtactc atggatgttt ctcatggcat
     2401 ttgccctggg ttggagatca tacagacaat caagtggaaa cctgctctgc tttgctcctg
     2461 atctgattat taatgagcag agaatgtctc taccctgcat gtatgaccaa tgtaaacaca
     2521 tgctgttcgt ctcctctgag ctccagcgat tgcaggtatc ctatgaagag tatctctgta
     2581 tgaaaacctt actgcttctc tcctcagttc ctaaggaagg tctgaagagc caagagttat
     2641 ttgatgagat tcgaatgact tatatcaaag agctaggaaa agccatcgtc aaaagggaag
     2701 ggaactccag tcagaactgg caacggtttt accaactgac aaagcttctg gactccatgc
     2761 atgaggtggt tgagaatctc cttacctact gcttccagac atttttggat aagaccatga
     2821 gtattgaatt cccagagatg ttagctgaaa tcatcactaa tcagatacca aagtactcaa
     2881 acggtaatat caagaagctt ctgtttcatc aaaaatctac tagcaaaccg gtaacgttat
     2941 acgacgtcgc tgaatacgcc ggcgtttctc atcaaaccgt ttctagagtg gttaaccagg
     3001 cttcacatgt tagcgctaaa acccgggaaa aagttgaagc tgccatggct gagctcaact
     3061 acatcccgaa ccgtgttgcg cagcagctgg ctggtaaaca aagcttgctg atcggtgtcg
     3121 cgacctcgag cttggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
     3181 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
     3241 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
     3301 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
     3361 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
     3421 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
     3481 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
     3541 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
     3601 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
     3661 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
     3721 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa
     3781 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     3841 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     3901 aaaccactag tggatcggaa ttcgccaatt ttaatcaaag tgggaatatt gctgatagct
     3961 cattgtcctt cactttcact aacagtagca acggtccgaa cctcataaca actcaaacaa
     4021 attctcaagc gctttcacaa ccaattgcct cctctaacgt tcatgataac ttcatgaata
     4081 atgaaatcac ggctagtaaa attgatgatg gtaataattc aaaaccactg tcacctggtt
     4141 ggacggacca aactgcgtat aacgcgtttg gaatcactac agggatgttt aataccacta
     4201 caatggatga tgtatataac tatctattcg atgatgaaga taccccacca aacccaaaaa
     4261 aagagtaagc tttgagaccc tgca
//
