LOCUS       Exported                4524 bp ds-DNA    circular SYN 17-JAN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    lvl0 AlcR
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   militzis
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 9, 2020 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      2..59
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
                     /note="color: #ffffff"
     rep_origin      complement(254..842)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1025..1119
                     /gene="
                     "
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
                     /note="color: #ffffff"
     CDS             complement(1140..1799)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1983..2026
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
                     /note="color: #ffffff"
     misc_feature    2029..2034
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
                     /note="This feature has 2 segments:
                        1: 2029 .. 2029 / #99ccff
                        2: 2030 .. 2034 / #99ccff"
     misc_feature    2042..2045
                     /note="AATG Overhang"
                     /note="color: #ff0000"
     exon            2043..4508
                     /note="AlcR"
                     /note="color: #708090"
     misc_feature    4509..4512
                     /note="GCTT Overhang"
                     /note="color: #ff0000"
     misc_feature    4513..4516
                     /note="TGAG Overlap"
                     /note="color: #ff0000"
     misc_feature    4520..1
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
                     /note="color: #a6ccff"
ORIGIN
        1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
       61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      241 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1021 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1081 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1141 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1201 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1261 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1321 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     1381 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1441 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1501 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1561 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1621 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1681 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1741 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     1921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     1981 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcggtctc
     2041 taatggcaga tacgcgccga cgccagaatc atagctgcga tccctgtcgc aagggcaagc
     2101 gacgctgtga tgccccggaa aatagaaacg aggccaatga aaacggctgg gtttcgtgtt
     2161 caaattgcaa gcgttggaac aaggattgta ccttcaattg gctctcatcc caacgctcca
     2221 aggcaaaagg ggctgcacct agagcgagaa caaagaaagc caggaccgca acaaccacca
     2281 gtgaaccatc aacttcagct gcaacaatcc ctacaccgga aagtgacaat cacgatgcgc
     2341 ctccagtcat aaactctcac gacgcgctcc cgagctggac tcaggggcta ctctcccacc
     2401 ccggcgacct tttcgatttc agccactctg ctattcccgc aaatgcagaa gatgcggcca
     2461 acgtgcagtc agacgcacct tttccgtggg atctagccat ccccggtgat ttcagcatgg
     2521 gccaacagct cgagaaacct ctcagtccgc tcagttttca agcagtcctt cttccgcccc
     2581 atagcccgaa cacggatgac ctcattcgcg agctggaaga gcagactacg gatccggact
     2641 cggttaccga tactaatagt gtacaacagg tcgctcaaga tggatcgcta tggtctgatc
     2701 ggcagtcgcc gctactgcct gagaacagtc tgtgcatggc ctcagacagc acagcacggc
     2761 gatatgcccg ttccacaatg acgaagaatc tgatgcgaat ctaccacgat agtatggaga
     2821 atgcactgtc ctgctggctg acagagcaca attgtccata ctccgaccag atcagctacc
     2881 tgccgcccaa gcagcgggcg gaatggggcc cgaactggtc aaacaggatg tgcatccggg
     2941 tgtgccggct agatcgcgta tctacctcat tacgcgggcg cgccctgagt gcggaagagg
     3001 acaaagccgc agcccgagcc ctgcatctgg cgatcgtagc ttttgcgtcg caatggacgc
     3061 agcatgcgca gaggggggct gggctaaatg ttcctgcaga catagccgcc gatgagaggt
     3121 ccatccggag gaacgcctgg aatgaagcac gccatgcctt gcagcacacg acagggattc
     3181 catcattccg ggttatattt gcgaatatca tcttttctct cacgcagagt gtgctggatg
     3241 atgatgagca gcacggtatg ggtgcacgtc tagacaagct actcgaaaat gacggtgcgc
     3301 ccgtgttcct ggaaaccgcg aaccgtcagc tttatacatt ccgacataag tttgcacgaa
     3361 tgcaacgccg cggtaaggct ttcaacaggc tcccgggagg atctgtcgca tcgacattcg
     3421 ccggtatttt cgagacaccg acgccgtcgt ctgaaagccc acagcttgac ccggttgtgg
     3481 ccagtgagga gcatcgcagt acattaagcc ttatgttctg gctagggatc atgttcgata
     3541 cactaagcgc tgcaatgtac cagcgaccac tcgtggtgtc agatgaggat agccagatat
     3601 catcggcatc tccaccaagg cgcggcgctg aaacgccgat caacctagac tgctgggagc
     3661 ccccgagaca ggtcccgagc aatcaagaaa agagcgacgt atggggcgac ctcttcctcc
     3721 gcacctcgga ctctctccca gatcacgaat cccacacaca aatctctcag ccagcggctc
     3781 gatggccctg cacctacgaa caggccgccg ccgctctctc ctctgcaacg cccgtcaaag
     3841 tcctcctcta ccgccgcgtc acgcagctcc aaaccctcct ctatcgcggc gccagccctg
     3901 cccgccttga agcggccatc cagagaacgc tctacgttta taatcactgg acagcgaagt
     3961 accaaccatt tatgcaggac tgcgttgcta accacgagct cctcccttcg cgcatccagt
     4021 cttggtacgt cattctagac ggtcactggc atctagccgc gatgttgcta gcggacgttt
     4081 tggagagcat cgaccgcgat tcgtactctg atatcaacca catcgacctt gtaacaaagc
     4141 taaggctcga taatgcacta gcagttagtg cccttgcgcg ctcttcactc cgaggccagg
     4201 agctggaccc gggcaaagca tctccgatgt atcgccattt ccatgattct ctgaccgagg
     4261 tggcattcct ggtagaaccg tggaccgtcg ttcttattca ctcgtttgcc aaagctgcgt
     4321 atatcttgct ggactgttta gatctggacg gccaaggaaa tgcactagcg gggtacctgc
     4381 agctgcggca aaattgcaac tactgcattc gggcgctgca atttctgggc aggaagtcgg
     4441 atatggcggc gctggttgcg aaggatttag agagaggttt gaatgggaaa gttgacagct
     4501 ttttgtaggc tttgagaccc tgca
//
