LOCUS       Exported                2927 bp ds-DNA    circular SYN 14-MAY-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    MethylAble Terminator (GCTT-CGCT)
SOURCE      synthetic DNA construct
  ORGANISM  PCR product
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   SynthSys Center
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 31, 2020 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="PCR product"
                     /mol_type="other DNA"
     misc_feature    12..15
                     /note="CGCT Overhang"
                     /note="color: #ff0000"
     misc_feature    23..28
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
                     /note="color: #a6ccff"
     terminator      29..86
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
                     /note="color: #ffffff"
     rep_origin      complement(281..869)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1052..1146
                     /gene="
                     "
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
                     /note="color: #ffffff"
     CDS             complement(1167..1826)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      2010..2053
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
                     /note="color: #ffffff"
     misc_feature    2056..2061
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
                     /note="This feature has 2 segments:
                        1: 2056 .. 2056 / #99ccff
                        2: 2057 .. 2061 / #99ccff"
     misc_feature    2070..2073
                     /note="GCTT Overhang"
                     /note="color: #ff0000"
     promoter        2082..2116
                     /note="J23106 promoter"
                     /note="color: #00ffff"
     RBS             2125..2136
                     /note="B0034 RBS"
                     /note="color: #ffff00"
     gene            2143..2811
                     /note="amilCP"
                     /note="color: #333399; direction: RIGHT"
     terminator      2816..2887
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     terminator      2903..3
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
                     /note="color: #ffffff"
ORIGIN
        1 gcgcggtctc gcgcttgaga ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
       61 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
      121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc
      301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1021 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
     1081 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     1141 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
     1201 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     1261 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     1321 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     1381 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     1441 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     1501 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     1561 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     1621 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     1681 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     1741 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     1801 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     1861 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     1921 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     1981 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     2041 taaggatgat ttctggaatt caggtctctg cttcgagacc gtttacggct agctcagtcc
     2101 taggtatagt gctagctact agagaaagag gagaaatact aaatgagtgt gatcgctaaa
     2161 caaatgacct acaaggttta tatgtcaggc acggtcaatg gacactactt tgaggtcgaa
     2221 ggcgatggaa aaggtaagcc ctacgagggg gagcagacgg taaagctcac tgtcaccaag
     2281 ggcggacctc tgccatttgc ttgggatatt ttatcaccac agtgtcagta cggaagcata
     2341 ccattcacca agtaccctga agacatccct gactatgtaa agcagtcatt cccggagggc
     2401 tatacatggg agaggatcat gaactttgaa gatggtgcag tgtgtactgt cagcaatgat
     2461 tccagcatcc aaggcaactg tttcatctac catgtcaagt tctctggttt gaactttcct
     2521 cccaatggac ctgtcatgca gaagaagaca cagggctggg aacccaacac tgagcgtctc
     2581 tttgcacgag atggaatgct gctaggaaac aactttatgg ctctgaagtt agaaggaggc
     2641 ggtcactatt tgtgtgaatt taaaactact tacaaggcaa agaagcctgt gaagatgcca
     2701 gggtatcact atgttgaccg caaactggat gtaaccaatc acaacaagga ttacacttcg
     2761 gttgagcagt gtgaaatttc cattgcacgc aaacctgtgg tcgcctaata agcttcaaat
     2821 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2881 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttct
//
