LOCUS       Exported                5261 bp ds-DNA     circular SYN 12-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2020 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..792)
                     /label=AmpR
                     /note="color: #b7e6d7; direction: LEFT"
     CDS             complement(793..861)
                     /label=AmpR
                     /note="color: #b7e6d7; direction: LEFT"
     promoter        complement(862..966)
                     /label=AmpR promoter
                     /note="color: #c6c9d1; direction: LEFT"
     rep_origin      complement(992..1447)
                     /direction=LEFT
                     /label=f1 ori
                     /note="color: #ffef86"
     primer_bind     1586..1604
                     /label=272
                     /note="color: black; sequence: GTTGTAAAACGACGGCCAG"
     primer_bind     1589..1605
                     /label=M13 fwd
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     1589..1605
                     /label=1305
                     /note="color: black; sequence: GTAAAACGACGGCCAGT"
     promoter        1615..1633
                     /label=T7 promoter
                     /note="color: #c6c9d1"
     primer_bind     1617..1669
                     /label=618
                     /note="color: black; sequence: 
                     atacgactcactatagggcgaattggagctGATGCAGTTTTTTTCAATATTGC"
     primer_bind     complement(1620..1652)
                     /label=623
                     /note="color: black; sequence: 
                     TGCATCAGCTCCAATTCGCCCTATAGTGAGTCG"
     primer_bind     complement(1620..1646)
                     /label=675
                     /note="color: black; sequence: AGCTCCAATTCGCCCTATAGTGAGTCG"
     misc_feature    1647..2452
                     /label=hom
                     /note="color: #b4abac"
     primer_bind     complement(1647..1676)
                     /label=1269
                     /note="color: black; sequence: 
                     TTATTGTGCAATATTGAAAAAAACTGCATC"
     primer_bind     1664..1688
                     /label=594
                     /note="color: black; sequence: TATTGCACAATAAAGGCAAAATACG"
     primer_bind     1667..1694
                     /label=596
                     /note="color: black; sequence: 
                     TGCACAATAAAGGCAAAATACGGACAAA"
     primer_bind     1676..1700
                     /label=586
                     /note="color: black; sequence: AAGGCAAAATACGGACAAATTAGAT"
     primer_bind     1679..1703
                     /label=595
                     /note="color: black; sequence: GCAAAATACGGACAAATTAGATGAT"
     primer_bind     1983..2004
                     /label=1444
                     /note="color: black; sequence: CCCACTGTACCTCTGTTATCCA"
     primer_bind     2220..2239
                     /label=1442
                     /note="color: black; sequence: AGTGATGGAGCTGTGGTTGA"
     primer_bind     2252..2271
                     /label=558
                     /note="color: black; sequence: AGACCATGAGGTCTGCGTTT"
     primer_bind     2330..2352
                     /label=554
                     /note="color: black; sequence: CAGGTATCCCAGTGACTTAGGAG"
     primer_bind     complement(2420..2452)
                     /label=RvA1616
                     /note="color: black; sequence: 
                     tgtccacgctgGATTTTCAAAACAGTTGTATGG"
     primer_bind     complement(2420..2452)
                     /label=1616
                     /note="color: black; sequence: 
                     tgtccacgctgGATTTTCAAAACAGTTGTATGG"
     primer_bind     2422..2470
                     /label=1617
                     /note="color: black; sequence: 
                     atacaactgttttgaaaatccagcgtggacaATGGTGAGCAAGGGCGAG"
     primer_bind     complement(2427..2446)
                     /label=1277
                     /note="color: black; sequence: CGCTGGATTTTCAAAACAGT"
     misc_feature    2453..3148
                     /label=mScarlet-I
                     /note="color: #f58a5e"
     primer_bind     complement(3129..3168)
                     /label=1618
                     /note="color: black; sequence: 
                     cacaaaccttgagtagccatCTTGTACAGCTCGTCCATGC"
     primer_bind     complement(3129..3163)
                     /label=687
                     /note="color: black; sequence: 
                     accttgagtagccatCTTGTACAGCTCGTCCATGC"
     primer_bind     3134..3166
                     /label=688
                     /note="color: black; sequence: 
                     gacgagctgtacaagATGGCTACTCAAGGTTTG"
     misc_feature    3149..3942
                     /label=hom
                     /note="color: #b4abac"
     primer_bind     3149..3178
                     /label=RvA1619
                     /note="color: black; sequence: 
                     ATGGCTACTCAAGGTTTGTGTCATTAAATC"
     primer_bind     3149..3178
                     /label=1619
                     /note="color: black; sequence: 
                     ATGGCTACTCAAGGTTTGTGTCATTAAATC"
     misc_feature    3149..3161
                     /label=exon2
                     /note="color: #b4abac"
     primer_bind     complement(3349..3368)
                     /label=1443
                     /note="color: black; sequence: TCCAGAAGCAGTCATCCAGA"
     primer_bind     3610..3629
                     /label=522
                     /note="color: black; sequence: TTTGATGGAGTTGGACATGG"
     primer_bind     3629..3648
                     /label=524
                     /note="color: black; sequence: GCCATGGAACCAGACAGAAA"
     primer_bind     complement(3699..3718)
                     /label=1445
                     /note="color: black; sequence: TGTGGTAGTGGCACCAGAAT"
     primer_bind     complement(3700..3719)
                     /label=521
                     /note="color: black; sequence: CTGTGGTAGTGGCACCAGAA"
     primer_bind     complement(3733..3752)
                     /label=523
                     /note="color: black; sequence: CCTCAGGATTGCCTTTACCA"
     primer_bind     complement(3762..3781)
                     /label=525
                     /note="color: black; sequence: CAGGACTTGGGAGGTATCCA"
     primer_bind     complement(3862..3886)
                     /label=587
                     /note="color: black; sequence: TCTCAAAACTGCATTCTGACTTTCA"
     primer_bind     complement(3909..3972)
                     /label=619
                     /note="color: black; sequence: 
                     ttctagagcggccgccaccgcggtggagctCTCTCTTTTCTTCACCACAACATTTTATT
                     TAAAC"
     primer_bind     3913..3942
                     /label=1272
                     /note="color: black; sequence: 
                     AAATAAAATGTTGTGGTGAAGAAAAGAGAG"
     primer_bind     complement(3922..3942)
                     /label=1271
                     /note="color: black; sequence: CTCTCTTTTCTTCACCACAAC"
     primer_bind     3936..3964
                     /label=622
                     /note="color: black; sequence: 
                     AAGAGAGAGCTCCACCGCGGTGGCGGCCG"
     misc_feature    3943..4049
                     /label=MCS
                     /note="color: #84b0dc"
     primer_bind     3943..3964
                     /label=674
                     /note="color: black; sequence: AGCTCCACCGCGGTGGCGGCCG"
     primer_bind     3966..3982
                     /label=SK primer
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(4016..4032)
                     /label=KS primer
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4046..4066)
                     /label=1279
                     /note="color: black; sequence: AAAGGGAACAAAAGCTGGGTA"
     primer_bind     complement(4060..4079)
                     /label=534
                     /note="color: black; sequence: ATTAACCCTCACTAAAGGGA"
     promoter        complement(4062..4080)
                     /label=T3 promoter
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(4101..4117)
                     /label=M13 rev
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    4125..4141
                     /label=lac operator
                     /note="color: #31849b"
     promoter        complement(4149..4155)
                     /label=lac promoter
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(4154..4173)
                     /label=1176
                     /note="color: black; sequence: ctttatgcttccggctcgta"
     promoter        complement(4156..4173)
                     /label=lac promoter
                     /note="color: #c6c9d1; direction: LEFT"
     promoter        complement(4174..4179)
                     /label=lac promoter
                     /note="color: #c6c9d1; direction: LEFT"
     protein_bind    4194..4215
                     /label=CAP binding site
                     /note="color: #31849b"
     primer_bind     complement(4381..4404)
                     /label=948
                     /note="color: black; sequence: TATTACCGCCTTTGAGTGAGCTGA"
     primer_bind     4382..4401
                     /label=1122
                     /note="color: black; sequence: CAGCTCACTCAAAGGCGGTA"
     primer_bind     complement(4494..4513)
                     /label=1123
                     /note="color: black; sequence: GCCTATGGAAAAACGCCAGC"
     rep_origin      complement(4503..5091)
                     /direction=LEFT
                     /label=ori
                     /note="color: #ffef86"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     1021 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     1081 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     1141 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     1201 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     1261 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     1321 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     1381 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     1441 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     1501 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     1561 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     1621 gactcactat agggcgaatt ggagctgatg cagttttttt caatattgca caataaaggc
     1681 aaaatacgga caaattagat gataagattt atataaattt ttaaaatatt gatcaaaata
     1741 tgtatccata ttggtaatat ttgtatttat aataaatcat tgctgtaaat ttgaacttag
     1801 aaaaatttta ctaataaagg tgcttttgtg ttgcaaactt tcatttgaaa agtaattttt
     1861 ctttgtacca aaaaatctaa aattcgctat tctagtcacc aaaatttgct ttatgaaaaa
     1921 taatttttga tggcactata tcagaaaaca acttgttaaa gaaaatgtgg agtttttaaa
     1981 atcccactgt acctctgtta tccaaagggg atctgtgaat ttttctgtga aaggttaaaa
     2041 aaggagagac ctttaggaat tcagagagca gctgattttt gaatagtgtt ttcccctccc
     2101 tggcttttat tattacaact ctgtgctttt tcatcaccat cctgaatatc tataattaat
     2161 atttatacta ttaataaaaa gacatttttg gtaaggagga gttttcactg aagttcagca
     2221 gtgatggagc tgtggttgag gtgtctggag gagaccatga ggtctgcgtt tcactaacct
     2281 ggtaaaagag gatatgggtt ttttttgtgg gtgtaatagt gacatttaac aggtatccca
     2341 gtgacttagg agtattaatc aagctaaatt taaatcctaa tgacttttga ttaacttttt
     2401 ttagggtatt tgaagtatac catacaactg ttttgaaaat ccagcgtgga caatggtgag
     2461 caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat
     2521 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2581 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc
     2641 ccctcagttc atgtacggct ccagggcctt catcaagcac cccgccgaca tccccgacta
     2701 ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2761 cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt
     2821 gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg
     2881 ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa
     2941 gatggccctg cgcctgaagg acggcggccg ctacctggcg gacttcaaga ccacctacaa
     3001 ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt tggacatcac
     3061 ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc
     3121 caccggcggc atggacgagc tgtacaagat ggctactcaa ggtttgtgtc attaaatctt
     3181 tagttactga attggggctc tgcttcgttg ccattaagcc agtctggctg agatccccct
     3241 gctttcctct ctccctgctt acttgtcagg ctaccttttg ctccattttc tgctcactcc
     3301 tcctaatggc ttggtgaaat agcaaacaag ccaccagcag gaatctagtc tggatgactg
     3361 cttctggagc ctggatgcag taccattctt ccactgattc agtgagtaac tgttaggtgg
     3421 ttccctaagg gattaggtat ttcatcactg agctaaccct ggctatcatt ctgcttttct
     3481 tggctgtctt tcagatttga ctttatttct aaaaatattt caatgggtca tatcacagat
     3541 tctttttttt taaattaaag taacatttcc aatctactaa tgctaatact gtttcgtatt
     3601 tatagctgat ttgatggagt tggacatggc catggaacca gacagaaaag cggctgttag
     3661 tcactggcag caacagtctt acctggactc tggaatccat tctggtgcca ctaccacagc
     3721 tccttctctg agtggtaaag gcaatcctga ggaagaggat gtggatacct cccaagtcct
     3781 gtatgagtgg gaacagggat tttctcagtc cttcactcaa gaacaagtag ctggtaagag
     3841 tattattttt cattgcctta ctgaaagtca gaatgcagtt ttgagaacta aaaagttagt
     3901 gtataatagt ttaaataaaa tgttgtggtg aagaaaagag agagctccac cgcggtggcg
     3961 gccgctctag aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata
     4021 ccgtcgacct cgaggggggg cccggtaccc agcttttgtt ccctttagtg agggttaatt
     4081 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4141 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4201 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4261 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4321 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4381 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4441 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4501 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4561 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4621 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4681 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4741 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4801 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4861 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4921 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     4981 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5041 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5101 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5161 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5221 aaatcaatct aaagtatata tgagtaaact tggtctgaca g
//
