LOCUS       Exported                5953 bp ds-DNA     circular UNA 15-JAN-2020
DEFINITION  Level 1 yeast expression plasmid (Saccharomyces).
ACCESSION   urn.local...j-8nxy2im
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 13, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..495
                     /label=pAGT572_pvx extraction (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:w-8jwmoca"
     misc_feature    1..4
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    8..10
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    37..495
                     /label=pAGT527 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:1i-7qxarsx"
     misc_feature    37..40
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     misc_feature    37..40
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    37..40
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    41..110
                     /label=PGal1
                     /note="Geneious type: promoter eukaryotic"
     misc_feature    81..86
                     /label=Ex BpiI
                     /label=nonstandard type:  motif
     misc_feature    82
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     misc_feature    105..110
                     /label=Ex-BpiI
                     /label=nonstandard type:  motif
     misc_feature    108
                     /label=T
                     /note="Geneious type: Editing History Replacement"
     misc_feature    111..491
                     /label=PGal1
                     /note="Geneious type: promoter eukaryotic"
     misc_feature    111..114
                     /label=Ligation
                     /label=nonstandard type:  ligation
     primer_bind     426..463
                     /label=Forward primer Sequencing
     misc_feature    492..1091
                     /label=pAGM1933 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:f-8jmaj5p"
     primer_bind     492..495
                     /label=SENSE_PRM
                     /label=nonstandard type:  primer
     misc_feature    492..495
                     /label=pAGM1957 (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:k-8jmal68"
     misc_feature    492..495
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    492..495
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    complement(497..502)
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
     misc_feature    538..661
                     /label=P lac prom
                     /note="Geneious type: promoter prokaryotic"
     misc_feature    630
                     /label=P(LAC)
                     /note="Geneious type: promoter prokaryotic"
     CDS             668..988
                     /label=ALPHA
     misc_feature    1081..1086
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
     misc_feature    1088..5953
                     /label=pAGT572_pvx extraction (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:w-8jwmoca"
     misc_feature    1088..1529
                     /label=pAGT535 (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:1m-7qxb4ps"
     misc_feature    1088..1091
                     /label=pAGM1957 (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:k-8jmal68"
     misc_feature    1088..1091
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(1088..1091)
                     /label=ANTISENSE_PRM
                     /label=nonstandard type:  primer
     misc_feature    1088..1091
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    1088..1091
                     /label=Ligation
                     /label=nonstandard type:  ligation
     terminator      1092..1525
                     /label=tDIT1
     primer_bind     complement(1126..1157)
                     /label=Reverse Primer Sequencing
     misc_feature    join(1526..5953,1..40)
                     /label=pAGT572 (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:22-7qxbaj8"
     misc_feature    1526..1529
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    1526..1529
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    1547..1549
                     /label=Ligation
                     /label=nonstandard type:  ligation
     primer_bind     complement(1550..1551)
                     /label=ANTISENSE_PRM
                     /label=nonstandard type:  primer
     misc_feature    1553..1556
                     /label=Ligation
                     /label=nonstandard type:  ligation
     rep_origin      1557..3028
                     /label=2  ori
     primer_bind     complement(1581..1606)
                     /label=Sequencing primer
     primer_bind     complement(1581..1606)
                     /label=Sequencing primer reverse
     misc_feature    3029..3032
                     /label=Ligation
                     /label=nonstandard type:  ligation
     CDS             3141..3998
                     /label=AP(R)
     variation       3857
                     /label=G to A (BsaI removed)
                     /label=nonstandard type:  mutation
     misc_feature    4043..4046
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    4202..4816
                     /label=rep - pMB1
     variation       4360
                     /label=C to T
                     /label=nonstandard type:  mutation
     rep_origin      4801
                     /label=ORI
     misc_feature    4831..4834
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    4835..4857
                     /label=ura10
     CDS             complement(4927..5727)
                     /label=URA3 delta DSEBB
     misc_feature    5056
                     /label=G>T
     primer_bind     complement(5933..5953)
                     /label=ura1
ORIGIN
        1 tggtcactct gtgaagacaa tgccgaattc ggatccggag acggattaga agccgccgag
       61 cgggtgacag ccctccgaag ggagactctc ctccgtgcgt cctcgtcgtc accggtcgcg
      121 ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga acaataaaga ttctacaata
      181 ctagctttta tggttatgaa gaggaaaaat tggcagtaac ctggccccac aaaccttcaa
      241 atgaacgaat caaattaaca accataggat gataatgcga ttagtttttt agccttattt
      301 ctggggtaat taatcagcga agcgatgatt tttgatctat taacagatat ataaatgcaa
      361 aaactgcata accactttaa ctaatacttt caacattttc ggtttgtatt acttcttatt
      421 caaatgtaat aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag
      481 gagaaaaaac caatgtgaga ccgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      541 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      601 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      661 aacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc
      721 ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      781 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      841 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      901 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
      961 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     1021 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1081 ggtctcagct ttaaagtaag agcgctacat tggtctacct ttttgttctt ttacttaaac
     1141 attagttagt tcgttttctt tttctcattt ttttatgttt cccccccaaa gttctgattt
     1201 tataatattt tatttcacac aattccattt aacagagggg gaatagattc tttagcttag
     1261 aaaattagtg atcaatatat atttgccttt cttttcatct tttcagtgat attaatggtt
     1321 tcgagacact gcaatggccc tagttgtcta agaggataga tgttactgtc aaagatgata
     1381 ttttgaattt caattgacgt aattaatgat actattaata atacagagcg tatatgaagt
     1441 attgcaaata acatgcacag ttcttttggg atgagaatga taatgaaagg cgaaggcggg
     1501 cgttcagaaa agcgttgcgg agtaacgctg caattgtctt ctgcacgaag tggccgttat
     1561 cgatgataag ctgtcaaaga tgagaattaa ttccacggac tatagactat actagatact
     1621 ccgtctactg tacgatacac ttccgctcag gtccttgtcc tttaacgagg ccttaccact
     1681 cttttgttac tctattgatc cagctcagca aaggcagtgt gatctaagat tctatcttcg
     1741 cgatgtagta aaactagcta gaccgagaaa gagactagaa atgcaaaagg cacttctaca
     1801 atggctgcca tcattattat ccgatgtgac gctgcagctt ctcaatgata ttcgaatacg
     1861 ctttgaggag atacagccta atatccgaca aactgtttta cagatttacg atcgtacttg
     1921 ttacccatca ttgaattttg aacatccgaa cctgggagtt ttccctgaaa cagatagtat
     1981 atttgaacct gtataataat atatagtcta gcgctttacg gaagagaatg tatgtatttc
     2041 ggttcctgga gaaactattg catctattgc ataggtaatc ttgcacgtcg catccccggt
     2101 tcattttctg cgtttccatc ttgcacttca atagcatatc tttgttaacg aagcatctgt
     2161 gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct
     2221 gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat
     2281 ctgtgcttca tttttgtaaa acaaaaatgc aacgcgacga gagcgctaat ttttcaaaca
     2341 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa
     2401 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct
     2461 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga
     2521 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc
     2581 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg
     2641 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc
     2701 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa
     2761 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg
     2821 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta
     2881 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg
     2941 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc
     3001 aatgtttgtg gaagcggtat tcgcaatgcc gagtgcgcgg aacccctatt tgtttatttt
     3061 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3121 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3181 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3241 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3301 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3361 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3421 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3481 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3541 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3601 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3661 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3721 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3781 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3841 gagccggtga gcgtggttct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3901 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3961 agatcgctga gataggtgcc tcactgatta agcattggta atttcgcgta tccaacggct
     4021 gcccaggcag ccgtttttat tcctgtcaga ccaagtttac tcatatatac tttagattga
     4081 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4141 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4801 aaacgccagc aacgcggcct ttttacggtt cctgcctttt tcaatgggta ataactgata
     4861 taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt
     4921 tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg
     4981 ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat
     5041 gtcagatcct gtagatacca catcatccac ggttctatac tgttgaccca atgcgtcacc
     5101 cttgtcatct aaacccactc cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc
     5161 acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tggcaatgtc
     5221 aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa
     5281 catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac
     5341 aatacctggg cccacgacac cgtgagcatt cgtaatgtct gcccattctg ctattctgta
     5401 tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcctc
     5461 gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat
     5521 ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc
     5581 ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt
     5641 ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc
     5701 acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg tttttgttct
     5761 gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat
     5821 atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga
     5881 atcaaaaaaa tttcaaagaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt
     5941 gaattgaaaa gct
//
