LOCUS       Exported                5142 bp ds-DNA     circular UNA 07-JAN-2020
DEFINITION  episomal expression plasmid; GAP promoter; Amp (R); Hyg (R).
ACCESSION   urn.local...n-bfya2fv
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 13, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..11
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    8..11
                     /label=Ligation
                     /label=nonstandard type:  ligation
     terminator      12..213
                     /label=tGAP
                     /note="/created_by=ppuellma"
     primer_bind     complement(48..75)
                     /label=tGAP_seq_rev
     misc_feature    577..1602
                     /label=Hygromycin Marker
                     /label=nonstandard type:  Marker
                     /note="/created_by=ppuellma"
     misc_feature    1810..2269
                     /label=panARS
                     /label=nonstandard type:  motif
                     /note="/created_by=ppuellma"
     misc_feature    2355..3215
                     /label=Ampicillin Marker
                     /label=nonstandard type:  Marker
                     /note="/created_by=ppuellma"
                     /note="/modified_by=ppuellma"
     rep_origin      3370..3989
                     /label=pBR322 ori
                     /note="/created_by=ppuellma"
     misc_feature    4045..4048
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        4060..4543
                     /label=pGAP
                     /note="/created_by=ppuellma"
     primer_bind     4476..4502
                     /label=pGAP_seq_for
     misc_feature    4554..4557
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    4801..4961
                     /label=lacZalpha
                     /label=nonstandard type:  Reporter
                     /note="/created_by=ppuellma"
ORIGIN
        1 ggtctcagct ttcgatttgt atgtgaaata gctgaaattc gaaaatttca ttatggctgt
       61 atctacttta gcgtattagg catttgagca ttggcttgaa caatgcgggc tgtagtgtgt
      121 caccaaagaa accattcggg ttcggatctg gaagtcctca tcacgtgatg ccgatctcgt
      181 gtattttatt ttcagataac acctgaggac ttttacattg tcttcaatac aggacatgga
      241 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
      301 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
      361 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacgtgcg agcagggaaa
      421 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
      481 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
      541 atacagttct cacatcacat ccgaacataa acaaccatgg gtaaaaagcc tgaactcacc
      601 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
      661 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
      721 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
      781 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
      841 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctccc tgaaaccgaa
      901 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
      961 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     1021 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     1081 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     1141 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     1201 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     1261 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     1321 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     1381 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     1441 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     1501 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     1561 agtggaaacc gacgccccag cactcgtccg agggcaaagg aataatcagt actgacaata
     1621 aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc
     1681 tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag
     1741 atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg
     1801 ctatactggg agttcaacat ctttggataa tatcagaatg agaaagaaca gatacgcagt
     1861 acgttttttg gtgagctctt tgcacttctt tagttctttc catcaatatc agttgcttat
     1921 gcacttatga ctaatattga tgtttaactt caatatcttt aaacttttgt tcttcccgac
     1981 gttcattaag aatactaata cactttaata attagtttaa tatttgtttc tatataatga
     2041 catttaatta aaaaagataa aatataaaaa catcataata actcaccaga ggttaagaac
     2101 aaaaaaacaa attagatatc tgctaatcca atatagttaa atcaatcttt ccttggtata
     2161 atgggtatat tacatatatt tcaaggaccg acactcctac caaatatcta aaatttacca
     2221 tattaacata acatgtatat aaacgtcaaa tcataatcag cactaccgaa tttttctaaa
     2281 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2341 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2401 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2461 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2521 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2581 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2641 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2701 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2761 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2821 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2881 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2941 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3001 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3061 gtgagcgtgg ttctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     3541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctgcct ttttcaatgg
     4021 gtaataactg atataattaa attgaacgga agacaaaacg gatccttttt tgtagaaatg
     4081 tcttggtgtc ctcgtccaat caggtagcca tctctgaaat atctggctcc gttgcaactc
     4141 cgaacgacct gctggcaacg taaaattctc cggggtaaaa cttaaatgtg gagtaatgga
     4201 accagaaacg tctcttccct tctctctcct tccaccgccc gttaccgtcc ctaggaaatt
     4261 ttactctgct ggagagcttc ttctacggcc cccttgcagc aatgctcttc ccagcattac
     4321 gttgcgggta aaacggaggt cgtgtacccg acctagcagc ccagggatgg aaaagtcccg
     4381 gccgtcgctg gcaataatag cgggcggacg catgtcatga gattattgga aaccaccaga
     4441 atcgaatata aaaggcgaac acctttccca attttggttt ctcctgaccc aaagacttta
     4501 aatttaattt atttgtccct atttcaatca attgaacaac tatcaaaaca caaaatgtga
     4561 gaccgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     4621 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     4681 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     4741 tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
     4801 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4861 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4921 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     4981 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     5041 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5101 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg ac
//
