LOCUS       LC334437                2151 bp    DNA     linear   SYN 21-MAY-2020
DEFINITION  Synthetic construct tFucci(SA)5 gene for Fucci variant, complete
            cds.
ACCESSION   LC334437
VERSION     LC334437.1
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1
  AUTHORS   Ando,R., Sakaue-Sawano,A., Shoda,K. and Miyawaki,A.
  TITLE     Two new coral fluorescent proteins of distinct colors for sharp
            visualization of cell-cycle progression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Sakaue-Sawano,A., Shoda,K. and Miyawaki,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2017) Contact:Asako Sakaue-Sawano RIKEN, Lab for
            Cell Function Dynamics; 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
                     /clone="tFucci(SA)5"
     gene            1..2151
                     /gene="tFucci(SA)5"
     CDS             1..2151
                     /gene="tFucci(SA)5"
                     /codon_start=1
                     /transl_table=11
                     /product="Fucci variant"
                     /protein_id="BBB21277.1"
                     /translation="MENVRRKTGIQTEMKTKLHMDGMVNGHSFEIKGEGKGSPYEGVQ
                     TMKLKVTKGAPLPFSIDILLPQCMYGSKPFIKYPENIPDYLKLSFPEGITWERTMTFE
                     DGAVCDVSNDSRLVGNCFIYTVKFQGVNFPLDGPVMQKKTRGWEPSTEVLYECDGWMR
                     GLVDIALKLENGGHYMCNFKTTYKSKKGLEVPPYHFVDHKLDLLSHNTDGATFEEFEQ
                     GEIAHAHLSKLAGYPSHWRPLEPSPARPALRAPASATSGSRKRARPPAAPGRDQARPP
                     ARRRLRLSVDEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTITGR
                     AKRGSGATNFSLLKQAGDVEENPGPREFMSKLQKGVEKEMKIKLTMNCTVNERNFVIT
                     GQGAGEPYDGTQTLYLTVEGGKTLNFSFDVLTPAFQYGNRAFTKYPGNIPDFFKQTFS
                     GGGYTWKRTMTYEDGGVSTVESDISVQGDCFHYKIQFNGKFPPHGPVMQKETVKWEPS
                     TEVMYKDDKNDGVLKGDVNMALLLKDGGHLRVDFNTSYIPKNKVEKMPDYHFVDHRIE
                     ILEKPEGRPVKLYAGAVARYSLLPEKNLNKGYPSHWRPLEMNPSMKQKQEEIKENIKN
                     SSVPRRTLKMIQPSASGSLVGRENELSAGLSKRKHRNDHLTSTTSSPGVIVPESSENK
                     NLGGVTQESFDLMIKENPSSQYWKEVAEKRRKAL"
ORIGIN      
        1 atggagaacg tcaggagaaa gacaggcatt cagacggaaa tgaaaacgaa gctccacatg
       61 gatggtatgg tgaatggaca cagctttgag atcaaagggg aggggaaggg aagcccgtac
      121 gagggggtgc agacaatgaa gctgaaggtt actaagggcg ccccacttcc cttctctata
      181 gacattcttt tgcctcagtg catgtatggc agcaaacctt tcattaaata tcctgagaac
      241 atccccgatt atttgaaact ctcatttcca gagggtatca cttgggagag gacaatgaca
      301 ttcgaggacg gcgcagtatg cgatgtatct aatgattcca gactggtcgg caattgcttt
      361 atctacactg tgaagtttca gggagtcaat tttccacttg atggccccgt tatgcaaaag
      421 aagacccgag gctgggaacc ctccactgaa gtgctgtacg aatgtgatgg gtggatgcgc
      481 gggttggtgg acatagctct gaaactggaa aacggtggac attatatgtg taattttaag
      541 accacctaca aatccaagaa aggtcttgag gtgccacctt accatttcgt ggatcataaa
      601 ctcgacctgc tcagtcataa caccgacgga gccacctttg aggagttcga acaaggtgaa
      661 atcgctcacg cgcacttgag taaactggcc ggatatccat cacactggcg gccgctcgag
      721 cccagccccg ccaggcccgc actccgcgcc ccggcctccg ctaccagtgg cagccgcaag
      781 cgcgcccgcc cgcccgccgc ccccggacgc gaccaggcca ggccaccggc ccgcaggaga
      841 ctgcggctgt cggtggacga ggtttccagc cccagtaccc ccgaggcccc agacatccca
      901 gcctgccctt ctccgggcca gaagataaag aaatccaccc cggcagcagg tcagccgccc
      961 cacctgacat ccgcgcagga ccaggacacc atcaccggtc gcgccaagcg cggcagcggc
     1021 gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc cggcccccgc
     1081 gaattcatga gtaaactcca gaaaggtgtg gagaaagaga tgaagattaa gctcactatg
     1141 aactgcaccg tgaacgaacg gaactttgtg atcacagggc agggggctgg ggaaccttat
     1201 gatggaactc agacacttta cctgacagtg gagggcggaa agacactgaa cttcagcttc
     1261 gatgtactga ccccagcctt tcagtacggc aatagggcgt ttaccaagta ccccggtaac
     1321 ataccagact tcttcaaaca gaccttcagt ggcgggggat acacatggaa aagaacaatg
     1381 acctatgaag acggcggcgt ctcaaccgtt gagtccgata tcagcgttca aggggactgc
     1441 ttccattata aaatacaatt caacggaaag ttcccccccc acggcccagt catgcaaaag
     1501 gaaacagtca aatgggaacc ttccaccgag gtgatgtaca aggacgataa aaacgatgga
     1561 gtgctgaagg gcgacgtgaa tatggccctg ctgttgaagg atggtggtca cctcagagtc
     1621 gatttcaaca cttcttatat tcccaaaaat aaggtggaga agatgccaga ttaccacttc
     1681 gtggaccacc ggatcgagat cctggagaag cccgaaggac gccctgtcaa gctctacgca
     1741 ggggctgtgg ccaggtacag cctgctgcct gaaaagaacc tgaataaggg atatccatca
     1801 cactggcggc cgctcgagat gaatcccagt atgaagcaga aacaagaaga aatcaaagag
     1861 aatataaaga atagttctgt cccaagaaga actctgaaga tgattcagcc ttctgcatct
     1921 ggatctcttg ttggaagaga aaatgagctg tccgcaggct tgtccaaaag gaaacatcgg
     1981 aatgaccact taacatctac aacttccagc cctggggtta ttgtcccaga atctagtgaa
     2041 aataaaaatc ttggaggagt cacccaggag tcatttgatc ttatgattaa agaaaatcca
     2101 tcctctcagt attggaagga agtggcagaa aaacggagaa aggcgctgta a
//

