LOCUS       LC334438                2178 bp    DNA     linear   SYN 21-MAY-2020
DEFINITION  Synthetic construct tFucci(CA)5 gene for Fucci variant, complete
            cds.
ACCESSION   LC334438
VERSION     LC334438.1
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1
  AUTHORS   Ando,R., Sakaue-Sawano,A., Shoda,K. and Miyawaki,A.
  TITLE     Two new coral fluorescent proteins of distinct colors for sharp
            visualization of cell-cycle progression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Sakaue-Sawano,A., Shoda,K. and Miyawaki,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2017) Contact:Asako Sakaue-Sawano RIKEN, Lab for
            Cell Function Dynamics; 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
                     /clone="tFucci(CA)5"
     gene            1..2178
                     /gene="tFucci(CA)5"
     CDS             1..2178
                     /gene="tFucci(CA)5"
                     /codon_start=1
                     /transl_table=11
                     /product="Fucci variant"
                     /protein_id="BBB21278.1"
                     /translation="MENVRRKTGIQTEMKTKLHMDGMVNGHSFEIKGEGKGSPYEGVQ
                     TMKLKVTKGAPLPFSIDILLPQCMYGSKPFIKYPENIPDYLKLSFPEGITWERTMTFE
                     DGAVCDVSNDSRLVGNCFIYTVKFQGVNFPLDGPVMQKKTRGWEPSTEVLYECDGWMR
                     GLVDIALKLENGGHYMCNFKTTYKSKKGLEVPPYHFVDHKLDLLSHNTDGATFEEFEQ
                     GEIAHAHLSKLAGYPSHWRPLEMEQRRVTDFFARRRPGPPRIAPPKLACRTPSPARPA
                     LRAPASATSGSRKRARPPAAPGRDQARPPARAAARLSVDEVSSPSTPEAPDIPACPSP
                     GQKIKKTGRAKRGSGATNFSLLKQAGDVEENPGPREFMSKLQKGVEKEMKIKLTMNCT
                     VNERNFVITGQGAGEPYDGTQTLYLTVEGGKTLNFSFDVLTPAFQYGNRAFTKYPGNI
                     PDFFKQTFSGGGYTWKRTMTYEDGGVSTVESDISVQGDCFHYKIQFNGKFPPHGPVMQ
                     KETVKWEPSTEVMYKDDKNDGVLKGDVNMALLLKDGGHLRVDFNTSYIPKNKVEKMPD
                     YHFVDHRIEILEKPEGRPVKLYAGAVARYSLLPEKNLNKGYPSHWRPLEMNPSMKQKQ
                     EEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAGLSKRKHRNDHLTSTTSSPGV
                     IVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAEKRRKAL"
ORIGIN      
        1 atggagaacg tcaggagaaa gacaggcatt cagacggaaa tgaaaacgaa gctccacatg
       61 gatggtatgg tgaatggaca cagctttgag atcaaagggg aggggaaggg aagcccgtac
      121 gagggggtgc agacaatgaa gctgaaggtt actaagggcg ccccacttcc cttctctata
      181 gacattcttt tgcctcagtg catgtatggc agcaaacctt tcattaaata tcctgagaac
      241 atccccgatt atttgaaact ctcatttcca gagggtatca cttgggagag gacaatgaca
      301 ttcgaggacg gcgcagtatg cgatgtatct aatgattcca gactggtcgg caattgcttt
      361 atctacactg tgaagtttca gggagtcaat tttccacttg atggccccgt tatgcaaaag
      421 aagacccgag gctgggaacc ctccactgaa gtgctgtacg aatgtgatgg gtggatgcgc
      481 gggttggtgg acatagctct gaaactggaa aacggtggac attatatgtg taattttaag
      541 accacctaca aatccaagaa aggtcttgag gtgccacctt accatttcgt ggatcataaa
      601 ctcgacctgc tcagtcataa caccgacgga gccacctttg aggagttcga acaaggtgaa
      661 atcgctcacg cgcacttgag taaactggcc ggatatccat cacactggcg gccgctcgag
      721 atggagcagc gccgcgtcac cgacttcttc gcgcgccgcc gccccgggcc cccccgcatc
      781 gcgccgccca agctggcctg ccgcaccccc agccccgcca ggcccgcact ccgcgccccg
      841 gcctccgcta ccagtggcag ccgcaagcgc gcccgcccgc ccgccgcccc cggacgcgac
      901 caggccaggc caccggcccg cgcggccgcg cggctgtcgg tggacgaggt ttccagcccc
      961 agtacccccg aggccccaga catcccagcc tgcccttctc cgggccagaa gataaagaaa
     1021 accggtcgcg ccaagcgcgg cagcggcgcc accaacttca gcctgctgaa gcaggccggc
     1081 gacgtggagg agaaccccgg cccccgcgaa ttcatgagta aactccagaa aggtgtggag
     1141 aaagagatga agattaagct cactatgaac tgcaccgtga acgaacggaa ctttgtgatc
     1201 acagggcagg gggctgggga accttatgat ggaactcaga cactttacct gacagtggag
     1261 ggcggaaaga cactgaactt cagcttcgat gtactgaccc cagcctttca gtacggcaat
     1321 agggcgttta ccaagtaccc cggtaacata ccagacttct tcaaacagac cttcagtggc
     1381 gggggataca catggaaaag aacaatgacc tatgaagacg gcggcgtctc aaccgttgag
     1441 tccgatatca gcgttcaagg ggactgcttc cattataaaa tacaattcaa cggaaagttc
     1501 cccccccacg gcccagtcat gcaaaaggaa acagtcaaat gggaaccttc caccgaggtg
     1561 atgtacaagg acgataaaaa cgatggagtg ctgaagggcg acgtgaatat ggccctgctg
     1621 ttgaaggatg gtggtcacct cagagtcgat ttcaacactt cttatattcc caaaaataag
     1681 gtggagaaga tgccagatta ccacttcgtg gaccaccgga tcgagatcct ggagaagccc
     1741 gaaggacgcc ctgtcaagct ctacgcaggg gctgtggcca ggtacagcct gctgcctgaa
     1801 aagaacctga ataagggata tccatcacac tggcggccgc tcgagatgaa tcccagtatg
     1861 aagcagaaac aagaagaaat caaagagaat ataaagaata gttctgtccc aagaagaact
     1921 ctgaagatga ttcagccttc tgcatctgga tctcttgttg gaagagaaaa tgagctgtcc
     1981 gcaggcttgt ccaaaaggaa acatcggaat gaccacttaa catctacaac ttccagccct
     2041 ggggttattg tcccagaatc tagtgaaaat aaaaatcttg gaggagtcac ccaggagtca
     2101 tttgatctta tgattaaaga aaatccatcc tctcagtatt ggaaggaagt ggcagaaaaa
     2161 cggagaaagg cgctgtaa
//

