LOCUS       pAAV-CaMKII-Jaws        7369 bp    DNA     circular UNA 12-FEB-2021
DEFINITION  .
SOURCE      Cloning vector pEGFP-N3 other sequences; artificial sequences; (Cloning vector pEGFP-N3 other sequences; artificial sequences; (unknown)).
  ORGANISM  Cloning vector pEGFP-N3 other sequences; artificial sequences;
            Cloning vector pEGFP-N3 other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13735852|
COMMENT     VNTDBDATE|-13735852|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-CaMKII-Jaws-KGC-tdTomato-ER2|
COMMENT     VNTAUTHORNAME|Amy|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Sequence Source: Cloning vector pEGFP-N3 other sequences; artificial sequences; (Cloning vector pEGFP-N3 other sequences; artificial sequences; (unknown))." 
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FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Geneious name: L-ITR"
     misc_feature    3805..4401
                     /vntifkey="21"
                     /label=WPRE
                     /note="Geneious name: WPRE"
     misc_feature    complement(4632..4772)
                     /vntifkey="21"
                     /label=Misc_Feature_4
                     /note="Geneious name: R-ITR"
     misc_feature    6620..7308
                     /vntifkey="21"
                     /label=rep\origin
     misc_feature    5689..6549
                     /vntifkey="21"
                     /label=Ampr
     misc_feature    3781..3804
                     /vntifkey="21"
                     /label=ER2
     exon            1441..2268
                     /vntifkey="61"
                     /label=Halo57\mutant
     misc_feature    2281..2340
                     /vntifkey="21"
                     /label=KGC
     promoter        137..1425
                     /vntifkey="29"
                     /label=CaMKII
     misc_feature    2356..>3780
                     /vntifkey="21"
                     /label=tdTomato\(codon\diversed)
     polyA_signal    4406..4631
                     /vntifkey="25"
                     /label=bGH-PolyA
BASE COUNT     1703 a      1867 c      2053 g      1746 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct gtcgaccatt atggccttag gtcacttcat ctccatgggg ttcttcttct 
      181 gattttctag aaaatgagat gggggtgcag agagcttcct cagtgacctg cccagggtca 
      241 catcagaaat gtcagagcta gaacttgaac tcagattact aatcttaaat tccatgcctt 
      301 gggggcatgc aagtacgata tacagaagga gtgaactcat tagggcagat gaccaatgag 
      361 tttaggaaag aagagtccag ggcagggtac atctacacca cccgcccagc cctgggtgag 
      421 tccagccacg ttcacctcat tatagttgcc tctctccagt cctaccttga cgggaagcac 
      481 aagcagaaac tgggacagga gccccaggag accaaatctt catggtccct ctgggaggat 
      541 gggtggggag agctgtggca gaggcctcag gaggggccct gctgctcagt ggtgacagat 
      601 aggggtgaga aagcagacag agtcattccg tcagcattct gggtctgttt ggtacttctt 
      661 ctcacgctaa ggtggcggtg tgatatgcac aatggctaaa aagcagggag agctggaaag 
      721 aaacaaggac agagacagag gccaagtcaa ccagaccaat tcccagagga agcaaagaaa 
      781 ccattacaga gactacaagg gggaagggaa ggagagatga attagcttcc cctgtaaacc 
      841 ttagaaccca gctgttgcca gggcaacggg gcaatacctg tctcttcaga ggagatgaag 
      901 ttgccagggt aactacatcc tgtctttctc aaggaccatc ccagaatgtg gcacccacta 
      961 gccgttacca tagcaactgc ctctttgccc cacttaatcc catcccgtct gttaaaaggg 
     1021 ccctatagtt ggaggtgggg gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt 
     1081 tcgcatcccc ttctccaacc ccctcagtac atcaccctgg gggaacaggg tccacttgct 
     1141 cctgggccca cacagtcctg cagtattgtg tatataaggc cagggcaaag aggagcaggt 
     1201 tttaaagtga aaggcaggca ggtgttgggg aggcagttac cggggcaacg ggaacagggc 
     1261 gtttcggagg tggttgccat ggggacctgg atgctgacga aggctcgcga ggctgtgagc 
     1321 agccacagtg ccctgctcag aagccccaag ctcgtcagtc aagccggttc tccgtttgca 
     1381 ctcaggagca cgggcaggcg agtggcccct agttctgggg gcagcgaatt cgccgccacc 
     1441 atgaccgccg tgagcaccac agccactacc gtgctgcagg ccacacagag cgacgtgctg 
     1501 caggagatcc agtccaactt cctgctgaat agctccatct gggtgaacat tgctctggcc 
     1561 ggagtggtca tcctgctgtt tgtggccatg gggagggatc tggaatcccc tagagctaag 
     1621 ctgatctggg tggccacaat gctggtgcca ctggtgtcta tttctagtta cgctggactg 
     1681 gccagtgggc tgactgtggg cttcctgcag atgccacctg gacacgctct ggccggacag 
     1741 gaggtgctga gcccatgggg ccggtatctg acatggactt tctccactcc catgatcctg 
     1801 ctggctctgg gactgctggc cgacaccgat attgccagcc tgttcaccgc catcacaatg 
     1861 gacattggca tgtgcgtgac aggactggcc gctgccctga tcactagctc ccatctgctg 
     1921 cgctgggtgt tctacggaat ttcttgtgct ttctttgtgg ccgtgctgta tgtgctgctg 
     1981 gtgcagtggc cagctgatgc tgaggctgct gggaccagtg aaatctttgg cactctgagg 
     2041 attctgaccg tggtgctgtg gctggggtac cctatcctgt tcgctctggg ctctgaggga 
     2101 gtggccctgc tgagtgtggg agtgaccagc tggggatact ccggactgga catcctggct 
     2161 aaatacgtgt tcgcctttct gctgctgaga tgggtggctg ccaatgaagg cacagtgtct 
     2221 gggagtggaa tgggaatcgg gtccggagga gctgctccag ccgacgatcg accggtagta 
     2281 aaatccagaa ttacttctga aggggagtat atccctctgg atcaaataga catcaatgtt 
     2341 gcacctgcag gggcagtgag taagggcgag gaagtgatca aagagttcat gcggtttaag 
     2401 gtgagaatgg aaggaagcat gaacggccac gagttcgaaa ttgagggaga aggagaggga 
     2461 cggccctacg agggcaccca gacagccaag ctgaaagtga caaagggcgg gcctctgcca 
     2521 ttcgcttggg acatcctgag cccacagttt atgtacggct ccaaggccta tgtgaaacat 
     2581 ccagctgaca ttcccgatta taagaaactg agcttccccg aggggtttaa gtgggaaaga 
     2641 gtgatgaact tcgaggacgg aggcctggtg actgtgaccc aggacagctc cctgcaggat 
     2701 gggaccctga tctacaaggt gaaaatgaga gggacaaatt ttccccctga tggacctgtg 
     2761 atgcagaaga aaactatggg atgggaggcc tccaccgaaa ggctgtatcc acgcgacggg 
     2821 gtgctgaaag gagaaatcca ccaggctctg aagctgaaag atgggggaca ttacctggtg 
     2881 gagttcaaga caatctacat ggccaagaaa cctgtgcagc tgccaggcta ctattacgtg 
     2941 gacacaaaac tggatatcac ttcacacaac gaggactaca ctattgtgga gcagtatgaa 
     3001 cggagcgagg ggagacacca tctgttcctg ggccatggga ctggaagtac cggctcaggg 
     3061 tctagtggaa ccgcctcaag cgaggataac aatatggctg tgatcaaaga gttcatgagg 
     3121 tttaaggtgc gcatggaggg cagcatgaat gggcacgaat ttgagattga aggagagggc 
     3181 gaagggaggc cttacgaggg cacacagact gccaagctga aagtgaccaa gggaggacca 
     3241 ctgcctttcg cttgggatat cctgtctcct cagtttatgt acggaagtaa ggcctatgtc 
     3301 aagcatcccg ctgacattcc tgattacaag aaactgtctt tcccagaggg ctttaagtgg 
     3361 gagagagtga tgaattttga agatggaggc ctggtgaccg tgacacagga ctcctctctg 
     3421 caggatggca ctctgatcta caaagtcaaa atgcgcggca ccaattttcc acccgatggg 
     3481 cccgtgatgc agaagaaaac aatggggtgg gaggccagca ctgaacggct gtatcctaga 
     3541 gacggagtgc tgaagggcga aatccaccag gccctgaagc tgaaagacgg cggccactac 
     3601 ctggtggagt tcaaaaccat ctacatggcc aagaaaccag tgcagctgcc cggctattac 
     3661 tatgtggaca ccaagctgga tatcacatcc cacaatgaag actacaccat tgtggaacag 
     3721 tatgagaggt ctgaaggacg ccaccatctg tttctgtacg gcatggatga gctgtataag 
     3781 ttctgctacg agaatgaagt gtaatccgga atcaacctct ggattacaaa atttgtgaaa 
     3841 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 
     3901 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 
     3961 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 
     4021 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 
     4081 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 
     4141 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 
     4201 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 
     4261 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 
     4321 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 
     4381 tttgggccgc ctccccgcct ggatcctgtg ccttctagtt gccagccatc tgttgtttgc 
     4441 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 
     4501 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 
     4561 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 
     4621 ggctctatgg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc 
     4681 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc 
     4741 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac 
     4801 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc 
     4861 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 
     4921 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 
     4981 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 
     5041 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag 
     5101 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 
     5161 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg 
     5221 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 
     5281 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca 
     5341 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 
     5401 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 
     5461 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta 
     5521 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat 
     5581 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 
     5641 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 
     5701 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 
     5761 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 
     5821 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 
     5881 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 
     5941 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 
     6001 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 
     6061 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 
     6121 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 
     6181 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 
     6241 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 
     6301 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 
     6361 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 
     6421 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 
     6481 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 
     6541 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 
     6601 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 
     6661 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 
     6721 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 
     6781 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 
     6841 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 
     6901 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 
     6961 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 
     7021 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 
     7081 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 
     7141 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 
     7201 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 
     7261 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 
     7321 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt 
//
