LOCUS       pAAV-Syn-Jaws-KG        6557 bp    DNA     circular UNA 12-FEB-2021
DEFINITION  .
SOURCE      Cloning vector pEGFP-N3 other sequences; artificial sequences; (Cloning vector pEGFP-N3 other sequences; artificial sequences; (unknown)).
  ORGANISM  Cloning vector pEGFP-N3 other sequences; artificial sequences;
            Cloning vector pEGFP-N3 other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13735890|
COMMENT     VNTDBDATE|-13735890|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-Syn-Jaws-KGC-tdTomato-ER2|
COMMENT     VNTAUTHORNAME|Amy|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV-Syn-Jaws-KGC-tdTomato-ER2" 0 6557 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "BamHI" "EcoRI" "HindIII" "XmaI" "PacI" "AgeI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6557 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 3590 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 615 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XmaI" "cccggg" 2 0 3 41 0 3906 0 3917 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 1460 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "WPRE" 21 0 0 2993 3589 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3805..4401") 
COMMENT        #7=(CFSignal (CObList) "rep origin" 21 0 0 5808 6496 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6620..7308") 
COMMENT        #8=(CFSignal (CObList) "Ampr" 21 0 0 4877 5737 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5689..6549") 
COMMENT        #9=(CFSignal (CObList) "ER2" 21 0 0 2969 2992 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3781..3804") 
COMMENT        #10=(CFSignal (CObList) "Halo57 mutant" 61 0 0 629 1456 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1441..2268") 
COMMENT        #11=(CFSignal (CObList) "KGC" 21 0 0 1469 1528 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2281..2340") 
COMMENT        #12=(CFSignal (CObList) "tdTomato (codon diversed)" 21 0 0 1544 2968 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2356..>3780") 
COMMENT        #13=(CFSignal (CObList) "bGH-PolyA" 25 0 0 3594 3819 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4406..4631") 
COMMENT        #14=(CFSignal (CObList) "Human Synapsin I promoter" 21 0 0 137 613 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "314..802") 
COMMENT        #15=(CFSignal (CObList) "ITR" 21 0 0 1 130 0 (CStringList) (CStringList) 
COMMENT             1 1 1 1 "1..130") 
COMMENT        #16=(CFSignal (CObList) "ITR" 21 0 1 3820 3960 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3820..3960)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #17=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #18=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                    "pAAV-Syn-Jaws-KGC-tdTomato-ER2" 2) 5 "" 0 4) 
COMMENT              #19=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pAAV-Syn-Jaws-KGC-tdTomato-ER2'" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "." 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/12/21 05:45PM" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/12/21 05:45PM" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6557 bp" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #28=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Division: UNA" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 4-FEB-2021" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Sequence Source: Cloning vector pEGFP-N3 other sequences; artificial sequences; (Cloning vector pEGFP-N3 other sequences; artificial sequences; (unknown))." 
COMMENT                          1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Cloning+vector+pEGFP-N3+other+sequences;+artificial+sequences;\">Cloning vector pEGFP-N3 other sequences; artificial sequences;(Cloning vector pEGFP-N3 other sequences; artificial sequences;)</a>" 
COMMENT                          1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Taxonomy: Cloning vector pEGFP-N3 other sequences; artificial sequences; vectors" 
COMMENT                          1))) "Standard Fields") 
COMMENT              #35=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #36=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #38=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #39=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #40=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1     End: 130 " 1) 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1..130" 1))) "ITR") 
COMMENT                            #43=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 137 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 137   End: 613 " 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   314..802" 1))) 
COMMENT                                 "Human Synapsin I promoter") 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1469 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1469  End: 1528" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2281..2340" 1))) "KGC") 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1544 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1544  End: 2968" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2356..>3780" 1))) 
COMMENT                                 "tdTomato (codon diversed)") 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2969 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2969  End: 2992" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3781..3804" 1))) "ER2") 
COMMENT                            #59=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2993 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2993  End: 3589" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3805..4401" 1))) "WPRE") 
COMMENT                            #63=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3820 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3820  End: 3960 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3820..3960)" 1))) "ITR") 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4877 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4877  End: 5737" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5689..6549" 1))) "Ampr") 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5808 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5808  End: 6496" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   6620..7308" 1))) "rep origin"))) 
COMMENT                          "Misc. Feature (9 total)") 
COMMENT                     #75=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 25 (7 25 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3594 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3594  End: 3819" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4406..4631" 1))) "bGH-PolyA"))) 
COMMENT                          "PolyA Signal (1 total)") 
COMMENT                     #80=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 61 (7 61 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 629 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 629   End: 1456" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1441..2268" 1))) "Halo57 mutant"))) 
COMMENT                          "Exon (1 total)"))) "Feature Map") 
COMMENT              #85=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #86=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 44910960 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #87=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1460 " 1))))) "AgeI: 1 site") 1 5 
COMMENT                          "ACCGGT" "TGGCCA") 
COMMENT                     #89=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 59249424 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #90=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 3590 " 1))))) "BamHI: 1 site") 1 5 
COMMENT                          "GGATCC" "CCTAGG") 
COMMENT                     #92=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 59254192 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #93=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 615 " 1))))) "EcoRI: 1 site") 1 5 
COMMENT                          "GAATTC" "CTTAAG") 
COMMENT                     #95=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 44899984 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" 
COMMENT                          "TTCGAA") 
COMMENT                     #96=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 44897872 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" 
COMMENT                          "AATTAATT") 
COMMENT                     #97=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 44918544 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #98=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 41 " 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                         " N2: 3906 " 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                         " N3: 3917 " 1))))) "XmaI: 3 sites") 1 
COMMENT                          5 "CCCGGG" "GGGCCC"))) "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #102=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #103=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #104=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #105=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #106=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #107=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #108=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #109=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #110=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #111=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #112=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #113=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #114=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #115=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #116=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #117=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #118=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #119=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #120=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #121=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #122=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #123=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #124=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #125=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #126=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #127=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #128=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #129=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #130=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #131=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #132=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #133=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #134=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #135=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #136=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #137=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #138=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #139=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #140=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #141=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 
COMMENT         -7049) 1 1 0 1 1 
COMMENT        (mapper: 16.3949 -14.357 40.0378 40.0378 0.01 10 -1 6557 6557 1 0 0) 
COMMENT        #142=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1701344367 100) 
COMMENT              (CObjectList 
COMMENT               #143=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -869 100) 
COMMENT                     (CObjectList 
COMMENT                      #144=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #108# 59423668 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27514 6.27314 1 0.0214037) 0.0956716) 
COMMENT                      #145=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #105# 1128350535 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "pAAV-Syn-Jaws-KGC-tdTomato-ER2" 
COMMENT                            "@N" 1 0 0.871165 0 -10 21.3298 1.39868 Nil) 
COMMENT                      #146=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #104# 1128482644 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "6557 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.52262 0.799245 Nil)) (CObjectList)) 
COMMENT               #147=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -939 100) 
COMMENT                     (CObjectList 
COMMENT                      #148=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -102 100) 
COMMENT                            (CObjectList 
COMMENT                             #149=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #107# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.83953 3.41069 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #150=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #103# 1414808391 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "WPRE" "@N" 1 
COMMENT                                   -2.49676 -15.681 0 -10 2.7474 1.04901 #149#) 
COMMENT                             #151=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #107# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 0.0583597 0.717537 
COMMENT                                    1 0.082322) 0.8 1.8 0) 
COMMENT                             #152=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #103# 1129595203 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "rep origin" 
COMMENT                                   "@N" 1 -8.62528 10.454 0 -10 4.24599 1.04901 
COMMENT                                   #151#) 
COMMENT                             #153=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #107# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 0.784507 1.60824 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #154=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #103# 60597848 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Ampr" "@N" 1 
COMMENT                                   -12.5738 5.22699 0 -10 2.39773 1.04901 #153#) 
COMMENT                             #155=(CScratch 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #111# 59178376 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 3.43365 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #156=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #103# 1094797127 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ER2" "@N" 1 
COMMENT                                   5.10265 -12.1963 0 -10 1.87323 1.04901 #155#) 
COMMENT                             #157=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #107# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 4.81132 4.86873 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #158=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #103# 1096041281 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "KGC" "@N" 1 
COMMENT                                   12.9776 0 0 -10 2.04806 1.04901 #157#) 
COMMENT                             #159=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #107# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.43365 
COMMENT                                    4.79697 1 0.082322) 0.8 1.8 0) 
COMMENT                             #160=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #103# 1094931540 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "tdTomato (codon diversed)" "@N" 1 16.1114 
COMMENT                                   -6.96932 0 -10 11.5641 1.04901 #159#) 
COMMENT                             #161=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #107# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 5.68672 6.14307 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #162=(CLabel (CWidget 0 (0 0) 1 2 0 0 #103# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Human Synapsin I promoter" "@N" 1 14.7215 
COMMENT                                   8.71165 0 -10 11.9887 1.04901 #161#) 
COMMENT                             #163=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #107# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 6.14881 6.27319 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #164=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #103# 921 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ITR" "@N" 1 
COMMENT                                   7.24013 10.454 0 -10 1.42365 1.04901 #163#) 
COMMENT                             #165=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #107# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.48459 2.61949 1 
COMMENT                                    0.082322) 0.8 1.8 1) 
COMMENT                             #166=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #103# 268632636 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ITR" "@N" 1 
COMMENT                                   -8.05622 -10.454 0 -10 1.42365 1.04901 #165#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #167=(CGroupWidget 
COMMENT                            (CWidget 25 (7 25 0) 1 2 0 0 Nil -311 100) 
COMMENT                            (CObjectList 
COMMENT                             #168=(CLine 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #122# 543716457 
COMMENT                                    100) (LOGPEN 0 6 32768) 8 0.835356 2.61949 
COMMENT                                   2.83571) 
COMMENT                             #169=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #103# 762249218 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "bGH-PolyA" "@N" 
COMMENT                                   1 -7.953 -12.1963 0 -10 4.67059 1.04901 #168#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #170=(CGroupWidget 
COMMENT                            (CWidget 61 (7 61 0) 1 2 0 0 Nil -521 100) 
COMMENT                            (CObjectList 
COMMENT                             #171=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #106# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 4.88021 5.67237 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #172=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #103# 1128350548 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Halo57 mutant" 
COMMENT                                   "@N" 1 14.1104 5.22699 0 -10 6.26908 1.04901 
COMMENT                                   #171#)) (CObjectList))) (CObjectList)) 
COMMENT               #173=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1148 100) 
COMMENT                     (CObjectList 
COMMENT                      #174=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -386 100) 
COMMENT                            (CObjectList 
COMMENT                             #175=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #110# 473203389 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.83953 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #176=(CLabel (CWidget 0 (0 0) 1 2 0 0 #112# 12 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Bam}HI (3590)" "@N (@S)" 5 -7.293 
COMMENT                                   -13.9386 0 -10 4.77049 0.799245 #175#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #177=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -1084 100) 
COMMENT                            (CObjectList 
COMMENT                             #178=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #110# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.68576 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #179=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #112# 30509184 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Eco}RI (615)" "@N (@S)" 5 12.4271 
COMMENT                                   6.96932 0 -10 3.79641 0.799245 #178#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #180=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -177 100) 
COMMENT                            (CObjectList 
COMMENT                             #181=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #110# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.87734 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #182=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #112# -800 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Age}I (1460)" "@N (@S)" 5 14.2779 
COMMENT                                   1.74233 0 -10 3.87134 0.799245 #181#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #183=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #3# 0) 1 2 0 0 Nil -1014 100) 
COMMENT                            (CObjectList 
COMMENT                             #184=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #110# 61520520 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 6.23492 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #185=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #102# 1768122726 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xma}I (41)" "@N (@S)" 5 3.35616 12.1963 
COMMENT                                   0 -10 3.37181 0.799245 #184#) 
COMMENT                             #186=(CScratch 
COMMENT                                   (CWidget 2 (1 #3# 2) 1 2 0 0 #110# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.53721 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #187=(CLabel (CWidget 0 (0 0) 1 2 0 0 #102# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xma}I (3906)" "@N (@S)" 5 -11.263 
COMMENT                                   -8.71165 0 -10 4.34589 0.799245 #186#) 
COMMENT                             #188=(CScratch 
COMMENT                                   (CWidget 3 (1 #3# 3) 1 2 0 0 #110# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.52669 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #189=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #102# 2082602632 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xma}I (3917)" "@N (@S)" 5 -12.61 
COMMENT                                   -6.96932 0 -10 4.07115 0.799245 #188#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #190=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -247 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #191=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -874 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 71862904 
COMMENT       (CStringList) 0 12955056 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey="21"
                     /label=ITR
                     /note="Geneious name: L-ITR"
     misc_feature    2993..3589
                     /vntifkey="21"
                     /label=WPRE
                     /note="Geneious name: WPRE"
     misc_feature    complement(3820..3960)
                     /vntifkey="21"
                     /label=ITR
                     /note="Geneious name: R-ITR"
     misc_feature    5808..6496
                     /vntifkey="21"
                     /label=rep\origin
     misc_feature    4877..5737
                     /vntifkey="21"
                     /label=Ampr
     misc_feature    2969..2992
                     /vntifkey="21"
                     /label=ER2
     exon            629..1456
                     /vntifkey="61"
                     /label=Halo57\mutant
     misc_feature    1469..1528
                     /vntifkey="21"
                     /label=KGC
     misc_feature    1544..2968
                     /vntifkey="21"
                     /label=tdTomato\(codon\diversed)
     polyA_signal    3594..3819
                     /vntifkey="25"
                     /label=bGH-PolyA
     misc_feature    137..613
                     /vntifkey="21"
                     /label=Human\Synapsin\I\promoter
BASE COUNT     1451 a      1725 c      1841 g      1540 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct gtcgacgcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta 
      181 ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca 
      241 agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca 
      301 ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc 
      361 cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg 
      421 gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag 
      481 ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg 
      541 gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc 
      601 tgagagcgca gtcgaattcg ccgccaccat gaccgccgtg agcaccacag ccactaccgt 
      661 gctgcaggcc acacagagcg acgtgctgca ggagatccag tccaacttcc tgctgaatag 
      721 ctccatctgg gtgaacattg ctctggccgg agtggtcatc ctgctgtttg tggccatggg 
      781 gagggatctg gaatccccta gagctaagct gatctgggtg gccacaatgc tggtgccact 
      841 ggtgtctatt tctagttacg ctggactggc cagtgggctg actgtgggct tcctgcagat 
      901 gccacctgga cacgctctgg ccggacagga ggtgctgagc ccatggggcc ggtatctgac 
      961 atggactttc tccactccca tgatcctgct ggctctggga ctgctggccg acaccgatat 
     1021 tgccagcctg ttcaccgcca tcacaatgga cattggcatg tgcgtgacag gactggccgc 
     1081 tgccctgatc actagctccc atctgctgcg ctgggtgttc tacggaattt cttgtgcttt 
     1141 ctttgtggcc gtgctgtatg tgctgctggt gcagtggcca gctgatgctg aggctgctgg 
     1201 gaccagtgaa atctttggca ctctgaggat tctgaccgtg gtgctgtggc tggggtaccc 
     1261 tatcctgttc gctctgggct ctgagggagt ggccctgctg agtgtgggag tgaccagctg 
     1321 gggatactcc ggactggaca tcctggctaa atacgtgttc gcctttctgc tgctgagatg 
     1381 ggtggctgcc aatgaaggca cagtgtctgg gagtggaatg ggaatcgggt ccggaggagc 
     1441 tgctccagcc gacgatcgac cggtagtaaa atccagaatt acttctgaag gggagtatat 
     1501 ccctctggat caaatagaca tcaatgttgc acctgcaggg gcagtgagta agggcgagga 
     1561 agtgatcaaa gagttcatgc ggtttaaggt gagaatggaa ggaagcatga acggccacga 
     1621 gttcgaaatt gagggagaag gagagggacg gccctacgag ggcacccaga cagccaagct 
     1681 gaaagtgaca aagggcgggc ctctgccatt cgcttgggac atcctgagcc cacagtttat 
     1741 gtacggctcc aaggcctatg tgaaacatcc agctgacatt cccgattata agaaactgag 
     1801 cttccccgag gggtttaagt gggaaagagt gatgaacttc gaggacggag gcctggtgac 
     1861 tgtgacccag gacagctccc tgcaggatgg gaccctgatc tacaaggtga aaatgagagg 
     1921 gacaaatttt ccccctgatg gacctgtgat gcagaagaaa actatgggat gggaggcctc 
     1981 caccgaaagg ctgtatccac gcgacggggt gctgaaagga gaaatccacc aggctctgaa 
     2041 gctgaaagat gggggacatt acctggtgga gttcaagaca atctacatgg ccaagaaacc 
     2101 tgtgcagctg ccaggctact attacgtgga cacaaaactg gatatcactt cacacaacga 
     2161 ggactacact attgtggagc agtatgaacg gagcgagggg agacaccatc tgttcctggg 
     2221 ccatgggact ggaagtaccg gctcagggtc tagtggaacc gcctcaagcg aggataacaa 
     2281 tatggctgtg atcaaagagt tcatgaggtt taaggtgcgc atggagggca gcatgaatgg 
     2341 gcacgaattt gagattgaag gagagggcga agggaggcct tacgagggca cacagactgc 
     2401 caagctgaaa gtgaccaagg gaggaccact gcctttcgct tgggatatcc tgtctcctca 
     2461 gtttatgtac ggaagtaagg cctatgtcaa gcatcccgct gacattcctg attacaagaa 
     2521 actgtctttc ccagagggct ttaagtggga gagagtgatg aattttgaag atggaggcct 
     2581 ggtgaccgtg acacaggact cctctctgca ggatggcact ctgatctaca aagtcaaaat 
     2641 gcgcggcacc aattttccac ccgatgggcc cgtgatgcag aagaaaacaa tggggtggga 
     2701 ggccagcact gaacggctgt atcctagaga cggagtgctg aagggcgaaa tccaccaggc 
     2761 cctgaagctg aaagacggcg gccactacct ggtggagttc aaaaccatct acatggccaa 
     2821 gaaaccagtg cagctgcccg gctattacta tgtggacacc aagctggata tcacatccca 
     2881 caatgaagac tacaccattg tggaacagta tgagaggtct gaaggacgcc accatctgtt 
     2941 tctgtacggc atggatgagc tgtataagtt ctgctacgag aatgaagtgt aatccggaat 
     3001 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 
     3061 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 
     3121 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 
     3181 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 
     3241 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 
     3301 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 
     3361 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 
     3421 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 
     3481 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 
     3541 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg atcctgtgcc 
     3601 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 
     3661 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 
     3721 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 
     3781 caatagcagg catgctgggg atgcggtggg ctctatggca ggaaccccta gtgatggagt 
     3841 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 
     3901 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg 
     3961 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 
     4021 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     4081 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc 
     4141 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     4201 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 
     4261 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     4321 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta 
     4381 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat 
     4441 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 
     4501 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 
     4561 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 
     4621 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 
     4681 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 
     4741 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     4801 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     4861 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     4921 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     4981 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     5041 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     5101 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     5161 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     5221 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     5281 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     5341 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     5401 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     5461 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     5521 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     5581 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     5641 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     5701 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     5761 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     5821 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     5881 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     5941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     6001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 
     6061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     6121 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     6181 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     6241 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     6301 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     6361 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     6421 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     6481 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     6541 gccttttgct cacatgt 
//
