LOCUS       pAAV-Syn-FLEX-rc        6281 bp    DNA     circular UNA 12-FEB-2021
DEFINITION  -.
ACCESSION   -
VERSION     .
KEYWORDS    GS63159 pAAV-Syn-FLEX-Chrimson_C170S-GFP.
SOURCE      null .
  ORGANISM  null .
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   AY
  TITLE     Direct Submission
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FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="null ."
                     /mol_type="genomic DNA"
                     /vntifkey="98"
     misc_feature    206..690
                     /vntifkey="21"
                     /label=Human\Synapsin\I\promoter
                     /note="L-ITR color: #a6acb3; direction: RIGHT"
     protein_bind    complement(699..732)
                     /bound_moiety="Cre recombinase"
                     /vntifkey="31"
                     /label=Lox2272
                     /note="lox2272 Cre-mediated recombination occurs in the 8-bp core  sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites. color: #31849b; direction: LEFT"
     protein_bind    complement(795..828)
                     /bound_moiety="Cre recombinase"
                     /vntifkey="31"
                     /label=LoxP
                     /note="loxP Cre-mediated recombination occurs in the 8-bp core  sequence (GCATACAT). color: #31849b; direction: LEFT"
     misc_feature    2693..3281
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE woodchuck hepatitis virus?posttranscriptional  regulatory element color: #ffffff"
     polyA_signal    3322..3529
                     /vntifkey="25"
                     /label=bGH_PA\term
                     /note="bGH poly(A) signal bovine growth hormone polyadenylation signal  color: #a6acb3"
     misc_feature    complement(3640..3817)
                     /vntifkey="21"
                     /label=ITR
                     /note="R-ITR color: #a6acb3; direction: LEFT"
     rep_origin      complement(4051..4739)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ori high-copy-number ColE1/pMB1/pBR322/pUC origin of  replication color: #ffff00"
     CDS             complement(4810..5670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=bla\CDS
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics This feature has 2 segments: ???1:?4993?..?5784?/?#ccffcc ???2:?5785?..?5853?/?#ccffcc?/?signal?sequence Cleavage site after base 5784"
     promoter        complement(5671..5775)
                     /gene="bla"
                     /vntifkey="30"
                     /label=bla
                     /note="AmpR promoter color: #ffffff; direction: LEFT"
     rep_origin      complement(5801..6256)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="f1 ori f1 bacteriophage origin of replication; arrow  indicates direction of (+) strand synthesis color: #ffff00"
     CDS             complement(842..1558)
                     /vntifkey="4"
                     /label=GFP
     misc_feature    complement(1577..2506)
                     /vntifkey="21"
                     /label=ChromeQ
     misc_feature    2523..2556
                     /vntifkey="21"
                     /label=Lox2272
     misc_feature    2627..2660
                     /vntifkey="21"
                     /label=LoxP
     misc_feature    1..192
                     /vntifkey="21"
                     /label=ITR
BASE COUNT     1411 a      1717 c      1668 g      1485 t 
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc 
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc 
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc 
      181 tctaggaaga tcgtaggtta attaagtgtc tagactgcag agggccctgc gtatgagtgc 
      241 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg 
      301 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg 
      361 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag 
      421 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga 
      481 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg 
      541 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc 
      601 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag 
      661 tcgtgtcgtg cctgagagcg cagtcgagaa gctagcccat aacttcgtat aaagtatcct 
      721 atacgaagtt atatcaaaat aggaagacca atgcttcacc atcgacccga attgccaagc 
      781 atcaccatcg acccataact tcgtataatg tatgctatac gaagttatac tagaattctc 
      841 attacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca 
      901 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt 
      961 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt 
     1021 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc 
     1081 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct 
     1141 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca 
     1201 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga 
     1261 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct 
     1321 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga 
     1381 gggtgggcca gggcacgggc agcttgccgg tggtgcaaat gaacttcagg gtcagcttgc 
     1441 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc 
     1501 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcactg 
     1561 ctactaccgg tgccgctggc acggctccgg cctcggcttc gtcttcgacg agagtctcga 
     1621 cctcgatctc cgttccgccg atgttcagtt tggtggtttt gcggatatct ccgtgaatca 
     1681 atatgtgctc gtggatcagg acgcgcaggt agtgtcccaa caacccccaa caatttttac 
     1741 tcatcagatc aataatcgtg tgacctacgg tggagccata gacgctcagg acgccaaaac 
     1801 cttcgggccc caaaatgaag agaattggga acatacccca gctcacgaaa aacagccatg 
     1861 ccatgccggt cacgacctgg cggcaccgac cctttggcac agtatgataa ccctcgatat 
     1921 atgctttggc ggcgtgaaaa aatgtgttcg cgccatagca caatccaaga caaaagaaga 
     1981 tgactttaac atagccggtt gccatggcgc tggtagcccc ccacacgata gtcccgatgt 
     2041 ctgagacaag gagtcccatg gttctcctgc tgtagtcgtt gctcaggccg gtgaggttgc 
     2101 tcaggtggat aaggatgaca ggacaagtga gcagccactc tgcatagcgc agccactgca 
     2161 ccgaacctcc tgtggcaagg tagagcatag agggattctt aaactcaaaa aagaactcga 
     2221 gaatcacctt aaccattgca atggcgcaca catagatctc ctcccagccg catgtagatt 
     2281 tccaggtttg gtaagaatag aacatcagca gcaaaatgct gaatcctgct gcaagccact 
     2341 gcaggacatt tgacgcggtc tgagcgccgt tcgtgccgcg agattcaatc catccggcac 
     2401 agtaacattg atcctcaggg accaggacgg acccgttcac caccacagga ttagtaacga 
     2461 acaaaagttc gcgtccgacg gcagacaaag cgccgccata gtccatggtg gcggatccta 
     2521 gtataacttc gtataggata ctttatacga agttatcatt gggattcttc ctattttgat 
     2581 ccaagcatca ccatcgaccc tctagtccag atctcaccat cgacccataa cttcgtatag 
     2641 catacattat acgaagttat gtccctcgaa gaggttcgcg aagcttatcg ataatcaacc 
     2701 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 
     2761 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 
     2821 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 
     2881 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 
     2941 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 
     3001 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 
     3061 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 
     3121 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 
     3181 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 
     3241 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacccgg 
     3301 gcggccgctc ataaagctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 
     3361 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 
     3421 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 
     3481 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtccgga 
     3541 ctgtacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa 
     3601 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atcttcctag agcatggcta 
     3661 cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt 
     3721 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg 
     3781 acgcccgggc tttgcccggg cggcctcagt gcagctgcat taatgaatcg gccaacgcgc 
     3841 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 
     3901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     3961 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     4021 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     4081 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     4141 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     4201 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     4261 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     4321 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     4381 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     4441 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 
     4501 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     4561 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     4621 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     4681 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     4741 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     4801 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     4861 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     4921 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     4981 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     5041 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     5101 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 
     5161 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 
     5221 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 
     5281 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 
     5341 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 
     5401 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 
     5461 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 
     5521 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 
     5581 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 
     5641 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 
     5701 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 
     5761 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt 
     5821 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     5881 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     5941 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     6001 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     6061 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     6121 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     6181 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     6241 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca a 
//
