LOCUS       pAAV-EF1a\1.1-FL        7032 bp    DNA     circular SYN 17-FEB-2021
DEFINITION  -.
ACCESSION   -
KEYWORDS    -.
SOURCE      - .
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13742718|
COMMENT     VNTDBDATE|-13742718|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-EF1a 1.1-FLEX-rc [ChromeQ-GFP]-bGH-PolyA-BE|
COMMENT     VNTAUTHORNAME|Amy|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                                          "pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point); pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point); pUC19 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point); pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" 
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COMMENT                                           "Required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein.; required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein; required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." 
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FEATURES             Location/Qualifiers
     rep_origin      complement(182..770)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point); pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point); pUC19 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point); pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=bla
                     /note="ampR (confers resistance to ampicillin)"
     sig_peptide     complement(1733..1801)
                     /gene="bla"
                     /vntifkey="94"
                     /label=bla
                     /note="Required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein.; required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein; required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein."
     promoter        complement(1843..1871)
                     /vntifkey="30"
                     /label=amp\prom
     repeat_region   2458..2647
                     /vntifkey="34"
                     /label=ITR
     protein_bind    complement(3848..3881)
                     /vntifkey="31"
                     /label=Lox2272
     protein_bind    complement(3944..3977)
                     /vntifkey="31"
                     /label=LoxP
     protein_bind    5672..5705
                     /vntifkey="31"
                     /label=Lox2272
     protein_bind    5776..5809
                     /vntifkey="31"
                     /label=LoxP
     enhancer        5841..6430
                     /vntifkey="9"
                     /label=WPRE
     polyA_signal    6471..6684
                     /vntifkey="25"
                     /label=bGH-PolyA
     repeat_region   complement(6789..6978)
                     /vntifkey="34"
                     /label=ITR
     misc_difference 2661..3839
                     /vntifkey="85"
                     /label=EF1a\Promoter\(1.1\Kb\short\version)
     CDS             complement(3991..4707)
                     /vntifkey="4"
                     /label=GFP
     misc_feature    complement(4726..5655)
                     /vntifkey="21"
                     /label=ChromeQ
BASE COUNT     1527 a      1868 c      1918 g      1719 t 
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt 
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg 
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag 
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag 
     2641 gaagatcgta ggttaattaa ggctccggtg cccgtcagtg ggcagagcgc acatcgccca 
     2701 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc 
     2761 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 
     2821 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 
     2881 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg 
     2941 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc 
     3001 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc 
     3061 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc 
     3121 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg 
     3181 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg 
     3241 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt 
     3301 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg 
     3361 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa 
     3421 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg 
     3481 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac 
     3541 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg 
     3601 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg 
     3661 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc 
     3721 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt 
     3781 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag 
     3841 ctagcccata acttcgtata aagtatccta tacgaagtta tatcaaaata ggaagaccaa 
     3901 tgcttcacca tcgacccgaa ttgccaagca tcaccatcga cccataactt cgtataatgt 
     3961 atgctatacg aagttatact agaattctca ttacttgtac agctcgtcca tgccgagagt 
     4021 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc 
     4081 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc 
     4141 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt 
     4201 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac 
     4261 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa 
     4321 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc 
     4381 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc 
     4441 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca 
     4501 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt 
     4561 ggtgcaaatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga 
     4621 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc 
     4681 ggtgaacagc tcctcgccct tgctcactgc tactaccggt gccgctggca cggctccggc 
     4741 ctcggcttcg tcttcgacga gagtctcgac ctcgatctcc gttccgccga tgttcagttt 
     4801 ggtggttttg cggatatctc cgtgaatcaa tatgtgctcg tggatcagga cgcgcaggta 
     4861 gtgtcccaac aacccccaac aatttttact catcagatca ataatcgtgt gacctacggt 
     4921 ggagccatag acgctcagga cgccaaaacc ttcgggcccc aaaatgaaga gaattgggaa 
     4981 cataccccag ctcacgaaaa acagccatgc catgccggtc acgacctggc ggcaccgacc 
     5041 ctttggcaca gtatgataac cctcgatata tgctttggcg gcgtgaaaaa atgtgttcgc 
     5101 gccatagcac aatccaagac aaaagaagat gactttaaca tagccggttg ccatggcgct 
     5161 ggtagccccc cacacgatag tcccgatgtc tgagacaagg agtcccatgg ttctcctgct 
     5221 gtagtcgttg ctcaggccgg tgaggttgct caggtggata aggatgacag gacaagtgag 
     5281 cagccactct gcatagcgca gccactgcac cgaacctcct gtggcaaggt agagcataga 
     5341 gggattctta aactcaaaaa agaactcgag aatcacctta accattgcaa tggcgcacac 
     5401 atagatctcc tcccagccgc atgtagattt ccaggtttgg taagaataga acatcagcag 
     5461 caaaatgctg aatcctgctg caagccactg caggacattt gacgcggtct gagcgccgtt 
     5521 cgtgccgcga gattcaatcc atccggcaca gtaacattga tcctcaggga ccaggacgga 
     5581 cccgttcacc accacaggat tagtaacgaa caaaagttcg cgtccgacgg cagacaaagc 
     5641 gccgccatag tccatggtgg cggatcctag tataacttcg tataggatac tttatacgaa 
     5701 gttatcattg ggattcttcc tattttgatc caagcatcac catcgaccct ctagtccaga 
     5761 tctcaccatc gacccataac ttcgtatagc atacattata cgaagttatg tccctcgaag 
     5821 aggttcgcga agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact 
     5881 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 
     5941 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 
     6001 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 
     6061 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 
     6121 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 
     6181 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 
     6241 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 
     6301 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 
     6361 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 
     6421 gcctccccgc atcgataccg tcgacccggg cggccgctca taaagctcga ctgtgccttc 
     6481 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
     6541 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
     6601 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
     6661 tagcaggcat gctggggatg cggtccggac tgtacaattg cattcatttt atgtttcagg 
     6721 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa 
     6781 tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt 
     6841 aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc 
     6901 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg 
     6961 agcgagcgag cgcgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 
     7021 gtattgggcg ct 
//
