LOCUS       LIC-Z.xdna    6937 bp      DNA     circular   15/05/2020
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: ps-CFP2-N from Evrogen. - [LOCUS]: Lck-PA-mChe
            rry-i
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     519..539
                     /label=CMV\Fwd
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="28" - label=CMVFwd - 519..539 \ 
     misc_feature    1033..1743
                     /label=mCherry 
                     /SerialCloner_Color=&hD40A13
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1765..3603
                     /label=CY2 
                     /SerialCloner_Color=&h42E51C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1739..1764
                     /label=GSTGSTGT linker
                     /SerialCloner_Color=&h44F1ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      6213..6841
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      3863..4303
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4836..5627
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        4602..4798
                     /label=SV40 early promoter
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        4370..4398
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4649..4726
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1733..1738
                     /label=BsrGI
                     /SerialCloner_Color=&hB874C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    625..654
                     /label=LCK10
                     /SerialCloner_Color=&h32AEC0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    655..669
                     /label=GGGD linker
                     /SerialCloner_Color=&h3BC049
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    613..618
                     /label=Xho I
                     /SerialCloner_Color=&hC01B21
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    670..1005
                     /label=TCR Cytoplasmic domain
                     /SerialCloner_Color=&h404CC0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagGC CACCatgggA tgtgtctgca gctcaaaccc tgaaggagga 
      661 ggaggggata gagtgaagtt cagcaggagc gcagagcccc ccgcgtacca gcagggccag 
      721 aaccagctct ataacgagct caatctagga cgaagagagg agtacgatgt tttggacaag 
      781 agacgtggcc gggaccctga gatgggggga aagccgagaa ggaagaaccc tcaggaaggc 
      841 ctgtacaatg aactgcagaa agataagatg gcggaggcct acagtgagat tgggatgaaa 
      901 ggcgagcgcc ggaggggcaa ggggcacgat ggcctttacc agggtctcag tacagccacc 
      961 aaggacacct acgacgccct tcacatgcag gccctgcccc ctcgcggttc tggaggcggt 
     1021 ggaagtggta ccATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC 
     1081 ATGCGCTTCA AGGTGCACAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC 
     1141 GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT 
     1201 GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC 
     1261 TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC 
     1321 AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC 
     1381 TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC 
     1441 GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC 
     1501 CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAA GGACGGCGGC 
     1561 CACTACGACG CTGAGGTCAA GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC 
     1621 GCCTACAACG TCAACATCAA GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG 
     1681 GAACAGTACG AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG 
     1741 GGCTCAACTG GAAGTACAGG AACAatgaag atggacaaaa agactatagt ttggtttaga 
     1801 agagacctaa ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt 
     1861 tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca 
     1921 agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct 
     1981 gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt 
     2041 accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac 
     2101 cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat 
     2161 ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat 
     2221 tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg 
     2281 cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta 
     2341 gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca 
     2401 ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat 
     2461 gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat 
     2521 ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg 
     2581 gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt 
     2641 ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg 
     2701 ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga 
     2761 caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga 
     2821 tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt 
     2881 ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt 
     2941 gacatccttg gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc 
     3001 ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa 
     3061 tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct 
     3121 ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta 
     3181 gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag 
     3241 atcatgatcg gagcagcacc tgatgagatt gtagcagata gcttcgaggc cttaggggct 
     3301 aataccatta aagaacctgg tctttgccca tctgtgtctt ctaatgacca acaagtacct 
     3361 tcggctgttc gttacaacgg gtcaaagaga gtgaaacctg aggaagaaga agagagagac 
     3421 atgaagaaat ctaggggatt cgatgaaagg gagttgtttt cgactgctga atcttcttct 
     3481 tcttcgagtg tgtttttcgt ttcgcagtct tgctcgttgg catcagaagg gaagaatctg 
     3541 gaaggtattc aagattcatc tgatcagatt actacaagtt tgggaaaaaa tggttgcaaa 
     3601 tgagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc 
     3661 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 
     3721 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     3781 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     3841 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 
     3901 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 
     3961 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 
     4021 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 
     4081 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 
     4141 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 
     4201 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 
     4261 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt 
     4321 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
     4381 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc 
     4441 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 
     4501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 
     4561 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 
     4621 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     4681 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     4741 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga 
     4801 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 
     4861 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 
     4921 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 
     4981 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 
     5041 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 
     5101 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 
     5161 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 
     5221 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 
     5281 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 
     5341 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 
     5401 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 
     5461 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 
     5521 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 
     5581 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 
     5641 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 
     5701 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 
     5761 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac 
     5821 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 
     5881 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 
     5941 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc 
     6001 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 
     6061 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta 
     6121 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
     6181 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     6241 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     6301 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     6361 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 
     6421 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     6481 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     6541 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     6601 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     6661 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     6721 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     6781 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     6841 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     6901 ccctgattct gtggataacc gtattaccgc catgcat
//