LOCUS       Exported                8366 bp ds-DNA     circular SYN 15-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 15, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            complement(96..956)
                     /label=Ampicillin
                     /note="/ApEinfo_fwdcolor=#f58a5e
                     /ApEinfo_revcolor=#f58a5e"
     promoter        complement(998..1026)
                     /label=AmpR_promoter
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     misc_feature    complement(1219..1525)
                     /label=f1_origin
                     /label=nonstandard type:  origin
                     /note="/ApEinfo_fwdcolor=#c7b0e3
                     /ApEinfo_revcolor=#c7b0e3"
     misc_feature    complement(1543..1703)
                     /label=lacZ_a
                     /label=nonstandard type:  feature
                     /note="/ApEinfo_fwdcolor=#d6b295
                     /ApEinfo_revcolor=#d6b295"
     promoter        1684..1700
                     /label=M13_forward20_primer
                     /note="/ApEinfo_fwdcolor=#faac61
                     /ApEinfo_revcolor=#faac61"
     promoter        1707..1725
                     /label=T7_promoter
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     misc_feature    1740..2052
                     /label=PB 5' LTR
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     promoter        2137..2440
                     /label=CMV enhancer
                     /note="/ApEinfo_fwdcolor=#c7b0e3
                     /ApEinfo_revcolor=#c7b0e3"
     promoter        2442..2719
                     /label=Chicken beta-actin promoter
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     misc_feature    2720..3732
                     /label=Chimeric intron
                     /note="/ApEinfo_fwdcolor=#d6b295
                     /ApEinfo_revcolor=#d6b295"
     gene            3793..5088
                     /label=Gfi1
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    5062..5085
                     /label=FLAG
                     /note="/ApEinfo_fwdcolor=#75c6a9
                     /ApEinfo_revcolor=#75c6a9"
     regulatory      5129..5713
                     /note="/ApEinfo_fwdcolor=#9eafd2
                     /ApEinfo_revcolor=#9eafd2
                     /label=IRES"
     gene            5720..6433
                     /label=EGFP
                     /note="/ApEinfo_fwdcolor=#9eafd2
                     /ApEinfo_revcolor=#9eafd2"
     misc_feature    5900..5929
                     /label=Y66 (EGFP)
                     /label=nonstandard type:  feature
                     /note="/ApEinfo_fwdcolor=#faac61
                     /ApEinfo_revcolor=#faac61"
     terminator      6448..6974
                     /label=rb_glob_PA_terminator
                     /note="/ApEinfo_fwdcolor=#9eafd2
                     /ApEinfo_revcolor=#9eafd2"
     misc_feature    7008..7242
                     /label=PB 3' LTR
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     promoter        complement(7261..7280)
                     /label=T3_promoter
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     promoter        complement(7297..7315)
                     /label=M13_reverse_primer
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     promoter        complement(7350..7379)
                     /label=lac_promoter
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
ORIGIN
        1 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
       61 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      121 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      181 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      241 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      301 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
      361 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
      421 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
      481 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
      541 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
      601 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
      661 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
      721 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
      781 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
      841 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
      901 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
      961 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1021 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1081 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     1141 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     1201 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     1261 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     1321 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     1381 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     1441 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     1501 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     1561 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     1621 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     1681 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacct
     1741 taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct
     1801 ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag
     1861 ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg
     1921 cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga
     1981 taattatatt gttatttcat gttctactta cgtgataact tattatatat atattttctt
     2041 gttatagata tcgtcgggtc gacattgatt attgactagt tattaatagt aatcaattac
     2101 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2161 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2221 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2281 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2341 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2401 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     2461 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     2521 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc
     2581 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
     2641 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     2701 gcgaagcgcg cggcgggcgg gagtcgctgc gttgccttcg ccccgtgccc cgctccgcgc
     2761 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     2821 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc tcgtttcttt
     2881 tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc cctttgtgcg ggggggagcg
     2941 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc ccgcgctgcc
     3001 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgcg tgtgcgcgag
     3061 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg ctgcgagggg aacaaaggct
     3121 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcggcg gtcgggctgt
     3181 aacccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     3241 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     3301 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     3361 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     3421 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     3481 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     3541 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     3601 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     3661 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     3721 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     3781 gaattgctcg agatgccgcg ctcattcctg gtcaagagca agaaggcgca cagctatcac
     3841 cagccgcgtt ctccggggcc ggactactcc ctgcgcctgg agaccgtgcc tgcgccgggc
     3901 agagcagagg gcggcgctgt gagtgcaggc gagtcgaaaa tggagccccg agagcgtttg
     3961 tcccccgact ctcagcttac cgaggctccc gacagggcct ccgcgtcccc caacagctgc
     4021 gaaggcagcg tttgtgaccc ctgctccgag ttcgaggact tttggaggcc cccttctccc
     4081 tccgtgtctc cagcgtcgga gaagtcactg tgccgctctc tggacgaagc ccagccctac
     4141 acgctgcctt tcaagcccta tgcatggagc ggtcttgctg ggtctgacct gcggcacctg
     4201 gtgcagagct atcggcagtg cagcgcgctg gagcgcagcg cgggcctgag cctcttctgc
     4261 gagcgcggct cggagccggg ccgcccggca gcgcgctacg gccccgagca ggctgcgggc
     4321 ggagccggtg cgggacagcc aggagactgc ggggtcgccg ggggcgccac cagcgctgcg
     4381 ggcctggggc tctacggcga cttcgcgcct gcggcggccg ggctgtacga gcggccgagc
     4441 acagcagcag gccggctgta ccaagatcat ggccacgagc tgcacgcgga caagagcgtg
     4501 ggcgtcaagg tggagtcgga gctgctttgc acccgtctgc tgctgggcgg cggctcctac
     4561 aaatgcatca aatgcagcaa ggtgttctcc acaccgcacg ggctggaggt gcacgtgcgc
     4621 cggtcccaca gcggcacaag accctttgcg tgcgagatgt gcggcaagac cttcgggcac
     4681 gcggtgagcc tggagcaaca caaggcagtg cactcccagg aacgcagctt tgactgtaag
     4741 atctgtggca agagcttcaa gaggtcatcc acgctgtcca cacatctgct cattcactcg
     4801 gacacccggc cctatccctg tcagtactgt ggcaaaagat tccaccagaa gtcagatatg
     4861 aagaaacaca ccttcatcca cacaggtgag aagccccaca aatgccaggt gtgcggcaaa
     4921 gccttcagtc agagctccaa cctcatcact catagcagaa agcacacagg cttcaagccc
     4981 tttggctgtg acctgtgtgg gaagggcttc cagaggaagg tggatctcag gaggcaccga
     5041 gagactcagc atggactcaa agactacaaa gacgatgacg acaagtgatg agaattctgc
     5101 agtcgacggt accgcgggcc cgggatccgc ccctctccct cccccccccc taacgttact
     5161 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     5221 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     5281 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     5341 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     5401 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     5461 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     5521 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     5581 tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta
     5641 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     5701 taatatggcc acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     5761 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     5821 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     5881 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     5941 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     6001 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     6061 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     6121 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     6181 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     6241 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     6301 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     6361 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     6421 cgagctgtac aagtaaagcg gccgcaattc actcctcagg tgcaggctgc ctatcagaag
     6481 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     6541 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     6601 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctatcact cggaaggaca
     6661 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     6721 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     6781 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     6841 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     6901 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     6961 ctcttatgga gatccctcga cctgcaccgt ctatggagat cggatccttt gttactttat
     7021 agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt
     7081 gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt
     7141 aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta
     7201 tctttaacgt acgtcacaat atgattatct ttctagggtt aaagagctcc agcttttgtt
     7261 ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7321 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7381 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7441 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7501 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7561 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7621 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7681 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7741 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7801 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7861 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7921 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7981 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8041 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8101 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     8161 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8221 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8281 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8341 actcacgtta agggattttg gtcatg
//
