LOCUS       Exported               11595 bp ds-DNA     circular SYN 30-APR-2020
DEFINITION  B synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   David Gordon
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 18, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virustype
                     1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to 
                     thecytoplasm."
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central 
                     terminationsequence of HIV-1 (lacking the first T)"
     promoter        2338..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human 
                     elongationfactor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon"
     misc_feature    3525..3540
                     /label=5' Homology with pLVX-EF1a-IRES-Puro
     misc_feature    3535
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3541..3549
                     /label=Kozak Consensus Sequence
     CDS             3550..5466
                     /codon_start=1
                     /label=nsp2
                     /translation="MAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDT
                     KRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKT
                     IQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCE
                     FCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGG
                     RTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVN
                     INIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKG
                     AWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSL
                     RLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKE
                     GVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIG
                     GAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEE
                     VVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFT
                     LKGG"
     CDS             5485..5508
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), anengineered 
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    5509..6318
                     /label=GGCGGTGGTTCAGGAGGAGGTAGCGGGGGTGGATCATGGTCACAT
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=GGCGGTGGTTCAGGAGGAGGTAGCGGGGGTGGATCA
                     TGGTCACAT"
     misc_feature    5509..6318
                     /label=ORF 2 (frame 1)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/codon_start=1"
                     /note="/created_by=ORF Finder"
     misc_feature    5509..5535
                     /label=HA Tag
                     /label=nonstandard type:  motif
                     /note="/Transferred_From=HA Tag"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     5509..5530
                     /label=1 F
     primer_bind     5509..5529
                     /label=1 F (1)
     primer_bind     5509..5528
                     /label=1 F (2)
     primer_bind     complement(5509..5523)
                     /label=r
     gene            5536..6315
                     /label=BASU*
                     /note="/created_by=User"
     gene            5536..5604
                     /label=BASU*
                     /note="/Transferred_From=Start BASU frag"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    5776..5781
                     /label=Split Site
                     /label=nonstandard type:  reporter
                     /note="/created_by=User"
     primer_bind     complement(6297..6318)
                     /label=810 R
     primer_bind     complement(6298..6318)
                     /label=810 R (1)
     primer_bind     complement(6299..6318)
                     /label=810 R (2)
     primer_bind     6304..6318
                     /label=f
     misc_feature    6307..6309
                     /label=E258S
                     /label=nonstandard type:  reporter
                     /note="/created_by=User"
     misc_feature    6313..6315
                     /label=G2R
                     /label=nonstandard type:  reporter
                     /note="/created_by=User"
     CDS             6319..6333
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), anengineered 
                     form of streptavidin"
                     /label=Strep-Tag II
                     /note="/Original_Translation=WSHPQFEK"
                     /translation="PQFEK"
     misc_feature    6337..6352
                     /label=3' Homology with pLVX-EF1a-IRES-Puro
     misc_feature    6338..6342
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    6345..6918
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6951..7550
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    7564..8152
                     /label=WPRE
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
     LTR             8359..8992
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9522..10110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10281..11141)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,and 
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11142..11246)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 gccgccacca tggcctacac gcgatatgtt gacaacaact tttgtggacc tgacggctac
     3601 cctcttgaat gtattaagga tcttctggct cgcgctggaa aagccagttg tacactgtct
     3661 gagcagctcg actttataga tacaaagcgg ggggtctatt gttgccgaga gcatgaacat
     3721 gagatcgcat ggtatacgga gcgctctgaa aaaagctatg aattgcagac accctttgaa
     3781 attaagttgg cgaaaaagtt cgatacattc aatggtgagt gccccaactt tgtctttccc
     3841 cttaacagta tcattaaaac aatccagccc cgggtggaaa agaagaaatt ggatggattc
     3901 atgggcagga tcagaagtgt ttacccagtg gcatccccta atgaatgcaa tcagatgtgc
     3961 ctgtccacgc tgatgaaatg cgaccactgc ggtgaaacgt cttggcaaac gggggacttt
     4021 gtaaaagcaa cgtgcgagtt ttgcggaact gagaacttga caaaggaggg agcgactaca
     4081 tgtggatacc tgcctcagaa cgcggtagtg aaaatatact gtcctgcctg tcacaattct
     4141 gaagttgggc ctgaacatag cttggccgaa taccataacg aaagtggatt gaagacaatt
     4201 cttcgcaaag gaggccggac tatcgcgttc gggggctgcg tgtttagcta tgtaggatgt
     4261 cataataaat gcgcatattg ggttccacga gctagcgcaa acataggctg caaccacaca
     4321 ggcgtagttg gtgagggatc agaaggtctt aatgataact tgctcgaaat attgcaaaag
     4381 gaaaaagtca acataaatat tgtaggagat tttaaattga atgaagagat tgcgataatc
     4441 cttgcgtcat tttccgcaag tacgagtgcg ttcgttgaga ctgtaaaggg gctcgactat
     4501 aaagcgttta agcaaatagt agagagctgc ggaaacttta aagttacgaa gggaaaagcc
     4561 aaaaaaggtg catggaacat tggcgagcag aagagtatac tgtcccccct gtacgccttc
     4621 gcgtcagaag cagctcgagt agttagaagt atattctcac gaacgctcga gacggcgcag
     4681 aatagtgtgc gcgtgctcca gaaggctgct attacgatcc tcgacggcat atcccagtat
     4741 tctcttcgac tgattgatgc tatgatgttc acctccgact tggcgacgaa caatctcgta
     4801 gttatggctt atatcaccgg cggcgttgta caattgacgt ctcagtggct gacgaacatc
     4861 ttcggaaccg tgtacgagaa gctcaaacca gttcttgatt ggcttgaaga aaagtttaag
     4921 gaaggcgtcg agtttctccg ggatgggtgg gaaattgtta agtttatctc cacctgcgcg
     4981 tgtgaaattg tgggagggca aattgtcacc tgcgccaagg aaattaaaga gtcagttcag
     5041 acgtttttta aacttgtgaa caaattcctt gctctctgtg cggatagtat aatcatcggt
     5101 ggggcgaagt tgaaagcact caatcttggt gaaactttcg tgacccacag caaaggcctg
     5161 tataggaagt gtgttaaaag cagggaggag accggtcttc ttatgccgct gaaggcgccc
     5221 aaagagataa tttttcttga gggggaaact cttccgaccg aagtgctcac ggaagaagta
     5281 gttctgaaaa caggggacct tcaaccactg gagcaaccga cctctgaggc agttgaagcg
     5341 ccgctcgtcg gaactcctgt atgtattaac ggcctcatgc tgttggaaat taaggatacg
     5401 gagaagtact gtgcactggc ccccaacatg atggtcacga acaacacctt tacccttaag
     5461 ggcggcctcg aaggcggcgg gggatggagc catccacaat tcgagaaata cccatacgat
     5521 gtgcctgact atgctggcaa gttgtctgaa tcagagatta ggtttggact gaaaacggag
     5581 gtgatgggcc aacatcttat ctatcacgat gttttgtcca gcacacaaaa gacagcccat
     5641 gaattggcaa ataacaatgc ccccgagggt accctcgtgg ttgcagataa acagacagcc
     5701 ggtaggggcg ggatgagccg agtgtggcat tctcaagaag gaaacggagt atggatgagt
     5761 ttgatattgc gcccggacat tccgctccaa aagacgcccc aacttacctt gctggcagct
     5821 gtagcggtgg ttcagggaat agaagaggcc gcgggcatac agactgatat aaagtggccg
     5881 aacgacatac tcattaacgg gaaaaaaaca gtagggatac ttaccgaaat gcaagctgag
     5941 gaggatcgag tgcgctccgt gattattggt attggcatta atgtaaatca gcaacccaat
     6001 gatttccccg atgagctcaa ggacattgcg acgagtctgt cccaggcagc tggggaaaag
     6061 atcgacaggg caggtgtaat ccagcatatc ttgctttgtt tcgaaaagcg ataccgagac
     6121 tacatgaccc acggtttcac gcccatcaag ctcttgtggg agtcatacgc cctcggtatt
     6181 ggcacgaaca tgagggcgag aactctcaac gggacatttt acggtaaggc gttgggtatt
     6241 gacgacgaag gggtactttt gttggaaact aatgaaggca ttaagaagat atacagcgcg
     6301 gacatcagcc ttaggtgacc gcagtttgaa aagtaaggat cccgcccctc tccctccccc
     6361 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     6421 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     6481 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     6541 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     6601 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     6661 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     6721 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     6781 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     6841 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     6901 tgaaaaacac gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt
     6961 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     7021 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     7081 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     7141 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     7201 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     7261 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     7321 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     7381 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     7441 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     7501 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg
     7561 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7621 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7681 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7741 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7801 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7861 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7921 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     7981 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8041 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8101 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     8161 aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     8221 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     8281 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8341 taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     8401 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     8461 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     8521 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     8581 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     8641 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     8701 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     8761 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     8821 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8881 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8941 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     9001 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     9061 aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg
     9121 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9181 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9241 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9301 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9361 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9421 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9481 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9541 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9601 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9661 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9721 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9781 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9841 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9901 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9961 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10021 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10081 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10141 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10201 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10261 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10321 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10381 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10441 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10501 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10561 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10621 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10681 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10741 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10801 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10861 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10921 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10981 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11041 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11101 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11161 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11221 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    11281 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    11341 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    11401 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    11461 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
    11521 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
    11581 tacaaactta gtagt
//
