LOCUS       Exported               10938 bp ds-DNA     circular SYN 30-APR-2020
DEFINITION  N Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Gordon DE, et al.
  TITLE     A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug 
            Targets and Potential Drug Repurposing
  JOURNAL   BioRxiv March 2020
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 18, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BioRxiv 
            March 2020"
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virustype
                     1"
     misc_feature    1181..2032
                     /label=ORF 1 (frame 2)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=2"
                     /note="/created_by=ORF Finder"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to 
                     thecytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central 
                     terminationsequence of HIV-1 (lacking the first T)"
     promoter        2338..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human 
                     elongationfactor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of 
                     humanEF-1-alpha"
     misc_feature    complement(2612..3259)
                     /label=ORF 8 (frame 3)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=3"
                     /note="/created_by=ORF Finder"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forwardprimer"
     misc_feature    3550..5679
                     /label=ORF 2 (frame 1)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/created_by=ORF Finder"
     CDS             4825..4848
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), anengineered 
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    4852..5661
                     /label=ORF 2 (frame 1)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/codon_start=1"
                     /note="/created_by=ORF Finder"
     misc_feature    4852..5661
                     /label=GGTGGTTCAGGAGGAGGTAGCGGGGGTGGATCATGGTCACAT
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=GGTGGTTCAGGAGGAGGTAGCGGGGGTGGATCATGG
                     TCACAT"
     misc_feature    4852..4878
                     /label=HA Tag
                     /label=nonstandard type:  motif
                     /note="/Transferred_From=HA Tag"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     4852..4873
                     /label=1 F
     primer_bind     4852..4872
                     /label=1 F (1)
     primer_bind     4852..4871
                     /label=1 F (2)
     primer_bind     complement(4852..4866)
                     /label=r
     gene            4879..5658
                     /label=BASU*
                     /note="/created_by=User"
     gene            4879..4947
                     /label=BASU*
                     /note="/Transferred_From=Start BASU frag"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    5119..5124
                     /label=Split Site
                     /label=nonstandard type:  reporter
                     /note="/created_by=User"
     primer_bind     complement(5640..5661)
                     /label=810 R
     primer_bind     complement(5641..5661)
                     /label=810 R (1)
     primer_bind     complement(5642..5661)
                     /label=810 R (2)
     primer_bind     5647..5661
                     /label=f
     misc_feature    5650..5652
                     /label=E258S
                     /label=nonstandard type:  reporter
                     /note="/created_by=User"
     misc_feature    5656..5658
                     /label=G2R
                     /label=nonstandard type:  reporter
                     /note="/created_by=User"
     CDS             5662..5676
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), anengineered 
                     form of streptavidin"
                     /label=Strep-Tag II
                     /note="/Original_Translation=WSHPQFEK"
                     /translation="PQFEK"
     misc_feature    5688..6261
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5855..5872)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse 
                     primer.Also called pCDH-rev"
     primer_bind     6082..6101
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6171..6893
                     /label=ORF 3 (frame 3)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=3"
                     /note="/created_by=ORF Finder"
     CDS             6294..6893
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6294..6313)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. 
                     Alsocalled puro-variant-R"
     primer_bind     6790..6810
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6907..7495
                     /label=WPRE
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
     primer_bind     complement(6960..6980)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7378..7389)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7702..8335
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8463..8479)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8463..8479)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8476..8498)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8487..8503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator 
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8511..8541)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8556..8577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the 
                     presenceof cAMP."
     primer_bind     complement(8694..8711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8865..9453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8945..8964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9624..10484)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,and 
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(9624..10484)
                     /label=ORF 7 (frame 2)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=2"
                     /note="/created_by=ORF Finder"
     primer_bind     10247..10266
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10485..10589)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10637..10771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10674..10693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10728..10747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 gccgccacca tgagcgataa cggcccccaa aaccaacgga atgcccctcg gataacattt
     3601 ggtggcccta gtgattccac aggaagtaac caaaacggag aacggtctgg agcccgctct
     3661 aaacagaggc gaccacaggg attgccaaat aacacagcgt catggtttac agccctgact
     3721 caacatggaa aggaggactt gaaatttcca cgaggacagg gtgtaccaat aaacacaaac
     3781 agtagtccag acgatcagat cgggtattat agaagggcca cgagacgcat cagaggtgga
     3841 gatggtaaaa tgaaagatct cagtccgcgc tggtactttt attatttggg aaccggccca
     3901 gaagcgggcc tcccatatgg agccaataag gacgggatta tatgggtggc tacagaggga
     3961 gccctcaata cgccaaaaga tcacatcgga acaagaaacc ctgctaataa tgccgcaatc
     4021 gtgctgcagt tgcctcaggg gacgacgctt cctaaaggct tttacgcaga aggatcacgc
     4081 ggcggcagcc aagcatcctc aaggtctagt tccagaagtc gaaacagctc cagaaactcc
     4141 acaccagggt ccagtagggg cacaagtccg gcgcggatgg cgggcaacgg cggagacgcc
     4201 gcactcgctc tgttgctcct ggaccggctc aaccaacttg aatccaagat gagtggtaag
     4261 ggacagcagc aacaaggtca aaccgtaacc aagaaaagcg ctgcagaagc ttctaaaaaa
     4321 cctcgacaaa aacggaccgc tacgaaggca tataacgtta ctcaagcctt cgggagacga
     4381 gggccggagc aaacccaggg gaattttgga gatcaggagc tcatccggca agggacagat
     4441 tacaaacatt ggcctcaaat cgctcagttc gcgccgtccg caagtgcttt tttcggaatg
     4501 tctcgcatcg gtatggaagt aacaccatcc gggacatggc tcacttatac cggcgccatc
     4561 aagctggacg acaaagatcc aaatttcaaa gatcaggtga ttctcttgaa taaacacata
     4621 gacgcgtata agacgtttcc acccaccgaa cccaagaagg acaaaaagaa aaaagctgat
     4681 gaaacacaag cgttgcccca gcgacagaag aagcagcaaa ccgtcaccct cctgccagcc
     4741 gctgatctcg atgacttctc caagcaactt caacagtcca tgagctcagc cgattctact
     4801 caggcgctcg aaggcggcgg gggatggagc catccacaat tcgagaaagg ctacccatac
     4861 gatgtgcctg actatgctgg caagttgtct gaatcagaga ttaggtttgg actgaaaacg
     4921 gaggtgatgg gccaacatct tatctatcac gatgttttgt ccagcacaca aaagacagcc
     4981 catgaattgg caaataacaa tgcccccgag ggtaccctcg tggttgcaga taaacagaca
     5041 gccggtaggg gcgggatgag ccgagtgtgg cattctcaag aaggaaacgg agtatggatg
     5101 agtttgatat tgcgcccgga cattccgctc caaaagacgc cccaacttac cttgctggca
     5161 gctgtagcgg tggttcaggg aatagaagag gccgcgggca tacagactga tataaagtgg
     5221 ccgaacgaca tactcattaa cgggaaaaaa acagtaggga tacttaccga aatgcaagct
     5281 gaggaggatc gagtgcgctc cgtgattatt ggtattggca ttaatgtaaa tcagcaaccc
     5341 aatgatttcc ccgatgagct caaggacatt gcgacgagtc tgtcccaggc agctggggaa
     5401 aagatcgaca gggcaggtgt aatccagcat atcttgcttt gtttcgaaaa gcgataccga
     5461 gactacatga cccacggttt cacgcccatc aagctcttgt gggagtcata cgccctcggt
     5521 attggcacga acatgagggc gagaactctc aacgggacat tttacggtaa ggcgttgggt
     5581 attgacgacg aaggggtact tttgttggaa actaatgaag gcattaagaa gatatacagc
     5641 gcggacatca gccttaggtg accgcagttt gaaaagtaag gatcccgccc ctctccctcc
     5701 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     5761 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     5821 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     5881 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     5941 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     6001 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     6061 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     6121 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     6181 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     6241 ctttgaaaaa cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg
     6301 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
     6361 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
     6421 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     6481 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6541 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6601 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6661 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6721 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6781 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6841 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tagacgcgtc
     6901 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6961 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7021 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7081 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7141 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7201 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7261 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7321 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7381 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7441 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     7501 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     7561 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     7621 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7681 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7741 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     7801 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7861 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     7921 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     7981 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     8041 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     8101 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     8161 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8221 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8281 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8341 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8401 gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca
     8461 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8521 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8581 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8641 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8701 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8761 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8821 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8881 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8941 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9001 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9061 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9121 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9181 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9241 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9301 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9361 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9421 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9481 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9541 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9601 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9661 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9721 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9781 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9841 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9901 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9961 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10021 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10081 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10141 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10201 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10261 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10321 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10381 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10441 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10501 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10561 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10621 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10681 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10741 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    10801 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    10861 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    10921 tactacaaac ttagtagt
//
