LOCUS       Exported               10347 bp ds-DNA     circular SYN 30-APR-2020
DEFINITION  M Krogan plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   David Gordon
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 18, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virustype
                     1"
     misc_feature    1181..2032
                     /label=ORF 1 (frame 2)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=2"
                     /note="/created_by=ORF Finder"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to 
                     thecytoplasm."
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central 
                     terminationsequence of HIV-1 (lacking the first T)"
     promoter        2338..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human 
                     elongationfactor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon"
     misc_feature    complement(2612..3259)
                     /label=ORF 10 (frame 3)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=3"
                     /note="/created_by=ORF Finder"
     primer_bind     3467..3487
                     /label=EF1a primer
     misc_feature    3525..3540
                     /label=5' Homology with pLVX-EF1a-IRES-Puro
     misc_feature    3535
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3541..3549
                     /label=Kozak Consensus Sequence
     misc_feature    3550..5088
                     /label=ORF 2 (frame 1)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/created_by=ORF Finder"
     CDS             3550..4215
                     /codon_start=1
                     /label=M
                     /translation="MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRF
                     LYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFA
                     RTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKD
                     LPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLNTDHSSSSDNIALLVQ
                     "
     CDS             4234..4257
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), anengineered 
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    4261..5070
                     /label=ORF 2 (frame 1)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/codon_start=1"
                     /note="/created_by=ORF Finder"
     misc_feature    4261..5070
                     /label=GGTGGTTCAGGAGGAGGTAGCGGGGGTGGATCATGGTCACAT
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=GGTGGTTCAGGAGGAGGTAGCGGGGGTGGATCATGG
                     TCACAT"
     misc_feature    4261..4287
                     /label=HA Tag
                     /label=nonstandard type:  motif
                     /note="/Transferred_From=HA Tag"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     4261..4282
                     /label=1 F
     primer_bind     4261..4281
                     /label=1 F (1)
     primer_bind     4261..4280
                     /label=1 F (2)
     primer_bind     complement(4261..4275)
                     /label=r
     gene            4288..5067
                     /label=BASU*
                     /note="/created_by=User"
     gene            4288..4356
                     /label=BASU*
                     /note="/Transferred_From=Start BASU frag"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    4528..4533
                     /label=Split Site
                     /label=nonstandard type:  reporter
                     /note="/created_by=User"
     primer_bind     complement(5049..5070)
                     /label=810 R
     primer_bind     complement(5050..5070)
                     /label=810 R (1)
     primer_bind     complement(5051..5070)
                     /label=810 R (2)
     primer_bind     5056..5070
                     /label=f
     misc_feature    5059..5061
                     /label=E258S
                     /label=nonstandard type:  reporter
                     /note="/created_by=User"
     misc_feature    5065..5067
                     /label=G2R
                     /label=nonstandard type:  reporter
                     /note="/created_by=User"
     CDS             5071..5085
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), anengineered 
                     form of streptavidin"
                     /label=Strep-Tag II
                     /note="/Original_Translation=WSHPQFEK"
                     /translation="PQFEK"
     misc_feature    5089..5104
                     /label=3' Homology with pLVX-EF1a-IRES-Puro
     misc_feature    5090..5094
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    5097..5670
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5580..6302
                     /label=ORF 3 (frame 3)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=3"
                     /note="/created_by=ORF Finder"
     CDS             5703..6302
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6316..6904
                     /label=WPRE
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
     misc_feature    6406..6933
                     /label=ORF 4 (frame 1)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/created_by=ORF Finder"
     LTR             7111..7744
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8274..8862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9033..9893)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,and 
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(9033..9893)
                     /label=ORF 9 (frame 2)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=2"
                     /note="/created_by=ORF Finder"
     promoter        complement(9894..9998)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 gccgccacca tggccgactc aaatgggacc attactgtag aggagctgaa aaaactgttg
     3601 gagcaatgga accttgtgat tggatttctc ttcttgacat ggatttgtct cctgcaattc
     3661 gcatacgcga atcgcaaccg attcctctat attataaaac ttatattcct ttggctcctg
     3721 tggcctgtga cccttgcctg cttcgtactc gcggcggtgt atcgaataaa ctggataact
     3781 ggcggcatag caatagctat ggcatgcctg gtaggtttga tgtggctctc ctattttata
     3841 gcgtcattca gactttttgc acgcacgcga agcatgtggt catttaaccc tgaaacaaat
     3901 atcttgctta atgtccccct gcatggcaca attttgaccc gaccgctcct tgaatcagaa
     3961 ttggtgattg gagccgtgat cctgaggggc cacttgagga tcgcaggaca tcatcttggt
     4021 cgctgtgata ttaaggattt gccaaaagaa ataactgtag ctaccagtcg aactttgagc
     4081 tattacaagc ttggagcgtc ccaaagggtc gctggcgata gtggatttgc agcctattca
     4141 cgctacagga tagggaacta taaactgaat acagaccact catcatcttc tgataacatt
     4201 gctttgctcg tccagctcga aggcggcggg ggatggagcc atccacaatt cgagaaaggc
     4261 tacccatacg atgtgcctga ctatgctggc aagttgtctg aatcagagat taggtttgga
     4321 ctgaaaacgg aggtgatggg ccaacatctt atctatcacg atgttttgtc cagcacacaa
     4381 aagacagccc atgaattggc aaataacaat gcccccgagg gtaccctcgt ggttgcagat
     4441 aaacagacag ccggtagggg cgggatgagc cgagtgtggc attctcaaga aggaaacgga
     4501 gtatggatga gtttgatatt gcgcccggac attccgctcc aaaagacgcc ccaacttacc
     4561 ttgctggcag ctgtagcggt ggttcaggga atagaagagg ccgcgggcat acagactgat
     4621 ataaagtggc cgaacgacat actcattaac gggaaaaaaa cagtagggat acttaccgaa
     4681 atgcaagctg aggaggatcg agtgcgctcc gtgattattg gtattggcat taatgtaaat
     4741 cagcaaccca atgatttccc cgatgagctc aaggacattg cgacgagtct gtcccaggca
     4801 gctggggaaa agatcgacag ggcaggtgta atccagcata tcttgctttg tttcgaaaag
     4861 cgataccgag actacatgac ccacggtttc acgcccatca agctcttgtg ggagtcatac
     4921 gccctcggta ttggcacgaa catgagggcg agaactctca acgggacatt ttacggtaag
     4981 gcgttgggta ttgacgacga aggggtactt ttgttggaaa ctaatgaagg cattaagaag
     5041 atatacagcg cggacatcag ccttaggtga ccgcagtttg aaaagtaagg atcccgcccc
     5101 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     5161 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     5221 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     5281 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     5341 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     5401 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     5461 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     5521 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     5581 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     5641 gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt
     5701 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     5761 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
     5821 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5881 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5941 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     6001 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     6061 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     6121 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     6181 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     6241 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     6301 agacgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6361 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6421 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6481 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6541 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6601 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6661 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     6721 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6781 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6841 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6901 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca
     6961 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     7021 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7081 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     7141 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     7201 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     7261 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     7321 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     7381 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     7441 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     7501 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     7561 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7621 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7681 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7741 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7801 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
     7861 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7921 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7981 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8041 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8101 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8161 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8221 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8281 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8461 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8701 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8761 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8821 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8881 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8941 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9001 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9061 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9121 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9181 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9241 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9301 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9361 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9421 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9481 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9541 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9601 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9661 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9721 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9781 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9841 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9901 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9961 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10021 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
    10081 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10141 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10201 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    10261 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    10321 ttaaatatgt actacaaact tagtagt
//
