LOCUS       Exported               10235 bp ds-DNA     circular SYN 15-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   HXN
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 18, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(246..556)
                     /label=5' TR long
                     /note="5' TR long"
     misc_feature    complement(377..406)
                     /label=SH-143
     primer_bind     576..600
                     /label=HJ73
     misc_feature    585..2256
                     /label=CAG promoter
     misc_feature    660..670
                     /label=CAG start
     promoter        966..1243
                     /label=chicken beta-actin promoter
     primer_bind     2178..2197
                     /label=YX-133
     primer_bind     complement(2232..2256)
                     /label=HJ74
     misc_feature    2262..2285
                     /label=169-170 anneal
     primer_bind     2286..2350
                     /label=4168F
     misc_feature    2286..2302
                     /label=atg
     primer_bind     2293..2325
                     /label=ZCY-5787
     primer_bind     2294..2313
                     /label=ZXX-20
     CDS             2303..2323
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     2323..2358
                     /label=4167F
     CDS             2333..5230
                     /codon_start=1
                     /label=mouse cas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     primer_bind     2333..2357
                     /label=ZCY-5848
     primer_bind     complement(3024..3043)
                     /label=ZCY-5596
     primer_bind     3029..3080
                     /label=ZCY-5595
     TATA_signal     3044..3061
                     /label=HEPN-1
     primer_bind     4239..4248
                     /label=ZCY-5596
     primer_bind     complement(4873..4900)
                     /label=ZCY-5598
     primer_bind     4884..4937
                     /label=ZCY-5597
     unsure          4901..4918
                     /label=HEPN-2
                     /note="higher eukarytoes and prokaryotes nucleotide-binding
                     domain"
     primer_bind     complement(5220..5239)
                     /label=ZCY-5849
     primer_bind     complement(5238..5266)
                     /label=ZCY-5788
     misc_feature    5240..5245
                     /label=Suntag
     primer_bind     complement(5246..5282)
                     /label=4167R
     CDS             5246..5266
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5261..5287)
                     /label=4168R
     primer_bind     complement(5266..5288)
                     /label=ZCY-5600
     misc_feature    5267
                     /label=Suntag
     misc_feature    5268..5272
                     /label=Suntag
     CDS             5273..5329
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5273..5329
                     /label=P2A
     misc_feature    5330
                     /label=Ab
                     /note="Nsi/ also used for replacing DsRed with sfGFP"
     CDS             5336..6055
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5367..5385
                     /label=gRNA site
     primer_bind     5404..5423
                     /label=Q-F
     primer_bind     complement(5567..5587)
                     /label=Q-R
     misc_feature    5988..6012
                     /label=TC1922
     misc_feature    6059..6073
                     /label=169-170 anneal
     polyA_signal    6113..6337
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6279..6298)
                     /label=hbb236
     primer_bind     complement(6319..6338)
                     /label=hu-pr49
     primer_bind     complement(6319..6338)
                     /label=hu-pr41
     misc_feature    complement(6347..6588)
                     /label=3'arm
     misc_feature    complement(6497..6526)
                     /label=SH-144
     misc_feature    6522..6540
                     /label=19
                     /label=19(1)
     misc_feature    6572..6584
                     /label=13
     misc_feature    6585..6588
                     /label=New Feature
                     /label=New Feature(1)
     misc_feature    6599
                     /label=TOPO cloning site
     promoter        complement(6669..6687)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6694..6710)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6695..6710
                     /label=M13F
     rep_origin      6851..7279
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             7623..8417
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8435..9295
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9466..10054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg
      301 ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc
      361 ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa
      421 cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc
      481 atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat
      541 tttcttgtta tagatatcat taatatcgat ggcgcgccgg attcgacatt gattattgac
      601 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      901 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      961 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     1021 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     1081 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
     1141 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     1201 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc
     1261 ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc
     1321 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     1381 ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga
     1441 gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     1501 gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     1561 ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     1621 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     1681 gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag
     1741 cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg
     1801 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     1861 ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     1921 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1981 tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag
     2041 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     2101 cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg
     2161 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     2221 accatgttca tgccttcttc tttttcctac agatccttaa ttaagcacgc gtgcgggatc
     2281 cgctcaattc gccgccacca tgcctaaaaa gaaaagaaag gtgggttctg gtatcgagaa
     2341 gaagaagagc ttcgccaagg gcatgggagt gaagagcacc ctggtgtccg gctctaaggt
     2401 gtacatgacc acatttgctg agggaagcga cgccaggctg gagaagatcg tggagggcga
     2461 tagcatcaga tccgtgaacg agggagaggc tttcagcgcc gagatggctg acaagaacgc
     2521 tggctacaag atcggaaacg ccaagttttc ccacccaaag ggctacgccg tggtggctaa
     2581 caacccactg tacaccggac cagtgcagca ggacatgctg ggactgaagg agacactgga
     2641 gaagaggtac ttcggcgagt ccgccgacgg aaacgataac atctgcatcc aggtcatcca
     2701 caacatcctg gatatcgaga agatcctggc tgagtacatc acaaacgccg cttacgccgt
     2761 gaacaacatc tccggcctgg acaaggatat catcggcttc ggaaagtttt ctaccgtgta
     2821 cacatacgac gagttcaagg atccagagca ccaccgggcc gcttttaaca acaacgacaa
     2881 gctgatcaac gccatcaagg ctcagtacga cgagttcgat aactttctgg ataaccccag
     2941 gctgggctac ttcggacagg ctttcttttc taaggagggc agaaactaca tcatcaacta
     3001 cggaaacgag tgttacgaca tcctggccct gctgagcgga ctgaggcact gggtggtgca
     3061 caacaacgag gaggagtctc ggatcagccg cacctggctg tacaacctgg acaagaacct
     3121 ggataacgag tacatctcca cactgaacta cctgtacgac aggatcacca acgagctgac
     3181 aaacagcttc tccaagaact ctgccgctaa cgtgaactac atcgctgaga ccctgggcat
     3241 caacccagct gagttcgctg agcagtactt cagattttcc atcatgaagg agcagaagaa
     3301 cctgggcttc aacatcacaa agctgagaga agtgatgctg gacagaaagg atatgtccga
     3361 gatcaggaag aaccacaagg tgttcgattc tatcagaacc aaggtgtaca caatgatgga
     3421 ctttgtgatc tacaggtact acatcgagga ggatgccaag gtggccgctg ccaacaagag
     3481 cctgcccgac aacgagaagt ctctgagcga gaaggatatc ttcgtgatca acctgagagg
     3541 ctcctttaac gacgatcaga aggacgctct gtactacgat gaggccaaca ggatctggag
     3601 aaagctggag aacatcatgc acaacatcaa ggagttccgg ggaaacaaga cccgcgagta
     3661 caagaagaag gacgctccaa ggctgcctag gatcctgcct gctggaaggg acgtgagcgc
     3721 cttcagcaag ctgatgtacg ccctgacaat gtttctggac ggaaaggaga tcaacgatct
     3781 gctgaccaca ctgatcaaca agttcgacaa catccagtct tttctgaaag tgatgcctct
     3841 gatcggcgtg aacgctaagt tcgtggagga gtacgccttc tttaaggaca gcgccaagat
     3901 cgctgatgag ctgcggctga tcaagtcctt tgccaggatg ggagagccaa tcgctgacgc
     3961 taggagagct atgtacatcg atgccatccg gatcctggga accaacctgt cttacgacga
     4021 gctgaaggct ctggccgaca ccttcagcct ggatgagaac ggcaacaagc tgaagaaggg
     4081 caagcacgga atgcgcaact tcatcatcaa caacgtgatc agcaacaagc ggtttcacta
     4141 cctgatcaga tacggcgacc cagctcacct gcacgagatc gctaagaacg aggccgtggt
     4201 gaagttcgtg ctgggacgga tcgccgatat ccagaagaag cagggccaga acggaaagaa
     4261 ccagatcgac cgctactacg agacctgcat cggcaaggat aagggaaagt ccgtgtctga
     4321 gaaggtggac gctctgacca agatcatcac aggcatgaac tacgaccagt tcgataagaa
     4381 gagatctgtg atcgaggaca ccggaaggga gaacgccgag agagagaagt ttaagaagat
     4441 catcagcctg tacctgacag tgatctacca catcctgaag aacatcgtga acatcaacgc
     4501 tagatacgtg atcggcttcc actgcgtgga gcgcgatgcc cagctgtaca aggagaaggg
     4561 atacgacatc aacctgaaga agctggagga gaagggcttt agctccgtga ccaagctgtg
     4621 cgctggaatc gacgagacag cccccgacaa gaggaaggat gtggagaagg agatggccga
     4681 gagagctaag gagagcatcg actccctgga gtctgctaac cctaagctgt acgccaacta
     4741 catcaagtac tccgatgaga agaaggccga ggagttcacc aggcagatca acagagagaa
     4801 ggccaagacc gctctgaacg cctacctgag gaacacaaag tggaacgtga tcatccggga
     4861 ggacctgctg cgcatcgata acaagacctg tacactgttc cggaacaagg ctgtgcacct
     4921 ggaggtggct cgctacgtgc acgcctacat caacgacatc gccgaggtga actcctactt
     4981 tcagctgtac cactacatca tgcagaggat catcatgaac gagagatacg agaagtctag
     5041 cggcaaggtg tctgagtact tcgacgccgt gaacgatgag aagaagtaca acgatagact
     5101 gctgaagctg ctgtgcgtgc ctttcggata ctgtatccca cggtttaaga acctgagcat
     5161 cgaggccctg ttcgaccgca acgaggctgc caagtttgat aaggagaaga agaaggtgag
     5221 cggcaactcc ggttctggtc tcgagcccaa gaagaagagg aaagtcctcg aggctactaa
     5281 cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggaccta tgcatatggt
     5341 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     5401 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     5461 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     5521 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     5581 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     5641 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5701 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5761 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     5821 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     5881 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5941 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     6001 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaacgct
     6061 agcgctgtcg acgccggtta ggggcccgtt taaacccgct gatcagcctc gactgtgcct
     6121 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6181 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6241 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6301 aatagcaggc atgctgggga tgcggtgggc tctatggcgg cgcgcctaaa agttttgtta
     6361 ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa cataaataaa
     6421 ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt aatatctatt
     6481 caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca attttacgca
     6541 tgattatctt taacgtacgt cacaatatga ttatctttct agggttaagg ccggccaaaa
     6601 gggcgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc
     6661 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     6721 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6781 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6841 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6901 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6961 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7021 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7081 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7141 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7201 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7261 caaaaattta acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga
     7321 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     7381 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     7441 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     7501 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     7561 caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc
     7621 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7681 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7741 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7801 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7861 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7921 aggatctcct gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7981 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     8041 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     8101 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     8161 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     8221 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     8281 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     8341 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     8401 ttgacgagtt cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8461 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8521 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     8581 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     8641 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8701 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8761 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8821 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8881 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8941 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     9001 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     9061 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     9121 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     9181 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     9241 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     9301 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     9361 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     9421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9601 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    10021 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    10081 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    10141 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    10201 cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
