LOCUS Exported 10562 bp ds-DNA circular SYN 10-OCT-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS 1279_Lenti-AcGFP-Sec61-IRES-Blast.dna SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10562) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 10, 2017 from SnapGene Viewer 4.0.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10562) /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 238..617) /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 835..1015) /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187) /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913) /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 2440..2557) /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2650..2861) /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" regulatory 2865..2874) /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2871..3587) /codon_start=1 /product="Aequorea coerulescens GFP" /label=AcGFP1 /note="mammalian codon-optimized" /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF GFVTAAAITHGMDELYK" misc_feature 3603..3893) /label=Sec61 intron 3945..4174) /label=chimeric intron /note="chimera between introns from adenovirus and immunoglobulin heavy chain genes" misc_feature 4232..4803) /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 4836..5234) /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 5254..5842) /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(5725..5736)) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5845..5861)) /label=KS primer /note="common sequencing primer, one of multiple similar variants" LTR 6367..6547) /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 6579..6803) /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6849..7277) /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 7291..7620) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7471..7606) /label=SV40 ori /note="SV40 origin of replication" promoter 7668..7715) /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7734..8108) /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8238..8359) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8408..8424)) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 8432..8448) /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8456..8486)) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8501..8522) /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8810..9398)) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9569..10429)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10430..10534)) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg 2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaggccacc atggtgagca 2881 agggcgccga gctgttcacc ggcatcgtgc ccatcctgat cgagctgaat ggcgatgtga 2941 atggccacaa gttcagcgtg agcggcgagg gcgagggcga tgccacctac ggcaagctga 3001 ccctgaagtt catctgcacc accggcaagc tgcctgtgcc ctggcccacc ctggtgacca 3061 ccctgagcta cggcgtgcag tgcttctcac gctaccccga tcacatgaag cagcacgact 3121 tcttcaagag cgccatgcct gagggctaca tccaggagcg caccatcttc ttcgaggatg 3181 acggcaacta caagtcgcgc gccgaggtga agttcgaggg cgataccctg gtgaatcgca 3241 tcgagctgac cggcaccgat ttcaaggagg atggcaacat cctgggcaat aagatggagt 3301 acaactacaa cgcccacaat gtgtacatca tgaccgacaa ggccaagaat ggcatcaagg 3361 tgaacttcaa gatccgccac aacatcgagg atggcagcgt gcagctggcc gaccactacc 3421 agcagaatac ccccatcggc gatggccctg tgctgctgcc cgataaccac tacctgtcca 3481 cccagagcgc cctgtccaag gaccccaacg agaagcgcga tcacatgatc tacttcggct 3541 tcgtgaccgc cgccgccatc acccacggca tggatgagct gtacaagtcc ggactcagat 3601 ctatgcctgg tccgaccccc agtggcacta acgtgggatc ctcagggcgc tctcccagca 3661 aagcagtggc cgcccgggcg gcgggatcca ctgtccggca gaggaaaaat gccagctgtg 3721 ggacaaggag tgcaggccgc acaacctcgg caggcaccgg ggggatgtgg cgattctaca 3781 cagaagattc acctgggctc aaagttggcc ctgttccagt attggttatg agtcttctgt 3841 tcatcgcttc tgtatttatg ttgcacattt ggggcaagta cactcgttcg tagcgatcca 3901 gtgtgctgga attaattcgc tgtctgcgag ggccagctgt tggggtgagt actccctctc 3961 aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat 4021 attcacctgg cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac 4081 aatctttttg ttgtcaagct tgaggtgtgg caggcttgag atctggccat acacttgagt 4141 gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag 4201 gtcgagcatg catctagggc ggccaattcc gcccctctcc cccccccccc taacgttact 4261 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata 4321 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt 4381 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa 4441 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag 4501 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca 4561 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc 4621 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat 4681 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta 4741 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga 4801 taagcttgcc acaacccaca aggagacgac cttccatggc caagcctttg tctcaagaag 4861 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact 4921 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat 4981 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg 5041 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc 5101 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga 5161 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg 5221 tgtgggaggg ctaacgatat caagcttatc ggtaatcaac ctctggatta caaaatttgt 5281 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5341 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 5401 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 5461 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 5521 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 5581 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 5641 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc 5701 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 5761 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 5821 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga 5881 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa 5941 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac 6001 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt 6061 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc 6121 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg 6181 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac 6241 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta 6301 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac 6361 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 6421 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 6481 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 6541 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc 6601 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 6661 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 6721 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 6781 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg 6841 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 6901 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 6961 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 7021 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 7081 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 7141 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 7201 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 7261 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 7321 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 7381 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 7441 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 7501 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct 7561 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 7621 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat 7681 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca 7741 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct 7801 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc 7861 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc 7921 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt 7981 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg 8041 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc 8101 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 8161 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 8221 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 8281 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 8341 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 8401 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 8461 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 8521 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 8581 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 8641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 8701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 8761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 8821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 8881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 8941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 9001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 9061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 9121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 9181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 9241 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 9301 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 9361 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 9421 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 9481 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 9541 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 9601 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 9661 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 9721 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 9781 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 9841 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 9901 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 9961 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 10021 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 10081 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 10141 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 10201 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 10261 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 10321 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 10381 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 10441 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 10501 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 10561 ac //