LOCUS       Exported                8265 bp ds-DNA     circular SYN 22-MAY-2020
DEFINITION  Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type 
            BAP1 Exon 7.
ACCESSION   .
VERSION     .
KEYWORDS    RG6-BAP1_E7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Pena-Llopis Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 22, 2020 from SnapGene 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             922..945
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     CDS             958..978
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     primer_bind     972..992
                     /label=Pr158 - RG6_RT-PCR_Fwd
                     /note="color: #cc99ff; direction: RIGHT"
     CDS             2427..3104
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 2427 .. 2429 / #ff0000 / 1a
                        2: 2430 .. 3104 / #ff0000"
                     /translation="VRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2445..2465)
                     /label=Pr159 - RG6_RT-PCR_Rev
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(2607..2627)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
                     /note="color: black; sequence: GTACTGGAACTGGGGGGACAG"
     primer_bind     2825..2844
                     /label=mCherry-F
                     /note="mCherry, forward primer"
                     /note="color: black; sequence: CCCCGTAATGCAGAAGAAGA"
     primer_bind     3019..3042
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
                     /note="color: black; sequence: AGCTGGACATCACCTCCCACAACG"
     CDS             3113..3829
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 3113 .. 3115 / #05fd14 / 1a
                        2: 3116 .. 3829 / #05fd14"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3155..3176)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     primer_bind     complement(3416..3435)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
                     /note="color: black; sequence: GTCTTGTAGTTGCCGTCGTC"
     primer_bind     3763..3784
                     /label=EGFP-C
                     /note="EGFP, forward primer"
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer_bind     complement(3855..3872)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    3861..4085
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      4131..4559
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(4218..4237)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     4428..4449
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     primer_bind     complement(4568..4588)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="color: black; sequence: ACCCTAACTGACACACATTCC"
     promoter        4573..4903
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4754..4889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     4816..4835
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     CDS             4970..5764
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5024..5043)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
                     /note="color: black; sequence: GCCCAGTCATAGCCGAATAG"
     primer_bind     5634..5653
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
                     /note="color: black; sequence: CGTTGGCTACCCGTGATATT"
     polyA_signal    5938..6059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(5975..5994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     6029..6048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     complement(6108..6124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6108..6124)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(6121..6143)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    6132..6148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6156..6186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6156 .. 6162 / #ffffff / -10
                        2: 6163 .. 6180 / #ffffff
                        3: 6181 .. 6186 / #ffffff / -35"
     protein_bind    6201..6222
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(6339..6356)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(6510..7098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(6590..6609)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(7269..8129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7269 .. 8060 / #ccffcc
                        2: 8061 .. 8129 / #ccffcc / signal sequence
                     Cleavage site after base 8060"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7892..7911
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(8130..8234)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttccat ggattacaag gatgacgatg acaagggggt acctgcccca
      961 aaaaaaaaac gcaaagtgga ggacccagta ccaggatcta gaggtaggtg atcctcctgc
     1021 tgctttggtt cagggttttg cttgaggggg gggggtggtg atttccttgc catgggcaga
     1081 ctgagcagaa aaggccattg ggaccatgtt ctgaatgcct ccacctcaac caccggccgg
     1141 taggaccaaa gccaccccgt gttttctcag gatctctttt cccagggaga tccctcggcc
     1201 caaagaggga gatggcaatg ctggatgtgt gcacaataat tcaacaggca ttggaacttc
     1261 agcatcgatg ctgaatgcaa ttaacaatgc tcaagcagaa cccccggctc catcagcaca
     1321 gtgcaggacc aaaccccatg ctgcagcagt ggggctgtct gtacggggtg ggcaatggga
     1381 accggggtct gctggggctc ctgctgcttc agtgctgcca tgcagccaca catcctgaga
     1441 gctgaaaggg tcggcgtcct cacctggtgc acaccgtagc tctgccccac agctttaagg
     1501 cacctggcta acctctgcgc ttcttccctt ccctcctccc tggctcagga tcccccgagc
     1561 cacgccacct ccctgagaag cagaatggcc ttagtgcagt gcggaccatg gaggcgttcc
     1621 actttgtcag ctatgtgcct atcacaggcc ggctctttga gctggatggg ctgaaggtct
     1681 accccattga ccatgaattc aggtagttac tgcacctttc tttgttccat ctctccacct
     1741 ctgctgtgaa taaatcgcgg gtcggtgtgt cctgtgcctt tccctgcttg ggaaacgctt
     1801 tcctttcatt ctttcacttc tctgctgctt tttgcgctct ccccatcctg ctgtgccaac
     1861 ctgctctcag ttctgtgctt tctgtcttcc atcccaacac acccctgggt tgctgtcttc
     1921 tttctccttt cttcctctct tgctgtggga ccaaacgtct cctgcaggac ctgcgggctc
     1981 tgacagagga ctctcgtggg ggtactgctc cctccagtgg aaaaatgctc cagcagtgtc
     2041 atgcaggaga tttatgccat acagttttgc tctctgctgc atggagggga gcagcagaag
     2101 tcgatctccc ccactctggg gtccccctcg aggggggcac agctggggag ggaacaaggg
     2161 acaaaaccag gagggggctc cgagtccttg gatttattcc ccctcatcca tgccttacct
     2221 tcaggtaagg gcctgaacag agccctttac ttcctgcttc tttctcccat agctccctct
     2281 ccttcgggtc tcctggactc agtgccacgg ttgtcccatt ctgggggtct gtagggagcc
     2341 agcaggagct gcggccgtcc tactgaccct gtccttattg cacaggtcag gaggatcagg
     2401 aggacgagga ggaagaggag accggtgtgc gctcctccaa gaacgtcatc aaggagttca
     2461 tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag atcgagggcg
     2521 agggcgaggg ccgcccctac gagggccaca acaccgtgaa gctgaaggtg accaagggcg
     2581 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt
     2641 acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca
     2701 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caagactcct
     2761 ccctgcagga cggctgcttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg
     2821 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
     2881 cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc
     2941 actacctggt ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct
     3001 actactacgt ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     3061 agcagtacga gcgcaccgag ggccgccacc acctgttcct gtagaccgcg gtgtgagcaa
     3121 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3181 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3241 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3301 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     3361 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3421 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3481 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3541 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     3601 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3661 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3721 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3781 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag ggcccgttta
     3841 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3901 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     3961 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4021 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4081 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4141 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4201 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4261 ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg
     4321 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4441 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4501 ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     4561 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag
     4621 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4681 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4741 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4801 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4861 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4921 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4981 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5041 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5101 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     5161 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5221 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5281 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5341 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5401 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5461 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5521 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5581 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5641 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5701 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5761 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5821 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5881 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     5941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6061 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6121 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6181 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6241 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6301 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6361 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6421 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6481 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6541 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6601 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6661 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     6721 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6781 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6841 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6901 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     6961 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7021 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7081 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7141 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7201 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7261 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7321 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7381 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7441 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7501 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7561 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7621 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7681 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7741 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7801 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7861 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7921 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7981 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8041 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8101 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8161 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8221 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
