LOCUS       Exported                2602 bp ds-DNA     circular UNA 19-MAY-2020
DEFINITION  backbone: pL0lacZ; SVP promoter with AC overhangs for uLoop  .
ACCESSION   urn.local...50-a4lpisa
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 20, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..40
                     /locus_tag="UNS1"
                     /label=UNS1
     misc_feature    42..47
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     misc_feature    complement(42..47)
                     /locus_tag="BsaI"
                     /label=BsaI
     misc_feature    46..601
                     /label=Phatr3_J16798_predictedprotein PCR Product
                     (con
                     /label=Phatr3_J16798_predictedprotein PCR Product
                     (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:2d-bumha16"
     misc_feature    46..52
                     /label=AC_Fprimer_extension
     misc_feature    46..48
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        53..594
                     /label=SVP promoter  (Phatr3_J16798)
                     
                     /note="/created_by=134729"
                     /note="/modified_by=13364144"
     misc_feature    53..594
                     /label=Phatr3_J16798_predictedprotein PCR Product
                     (con
                     /label=Phatr3_J16798_predictedprotein PCR Product
                     (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:2d-bumha16"
     misc_feature    complement(595..601)
                     /label=AC_Rprimer_extension
     misc_feature    join(599..2602,1..48)
                     /label=pL0R_lacZ (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:2e-bumha16"
     misc_feature    599..601
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    complement(600..605)
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     misc_feature    600..605
                     /locus_tag="BsaI(1)"
                     /label=BsaI
                     /label=BsaI(1)
     misc_feature    607..646
                     /locus_tag="UNSX"
                     /label=UNSX
     misc_feature    647..676
                     /locus_tag="Extra region of pBR322"
                     /label=Extra region of pBR322
     rep_origin      complement(737..1325)
                     /direction=LEFT
                     /locus_tag="pMB1-pUC19 ORI variant"
                     /label=pMB1-pUC19 ORI variant
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     misc_feature    complement(1416..1850)
                     /locus_tag="SpecR"
                     /label=SpecR
     misc_feature    complement(1416..1850)
                     /locus_tag="Spec gene(1)"
                     /label=Spec gene
                     /label=Spec gene(1)
     misc_feature    complement(1416..1850)
                     /locus_tag="pro-CDS-Ter of SpecR(1)"
                     /label=pro-CDS-Ter of SpecR
                     /label=pro-CDS-Ter of SpecR(1)
     misc_feature    complement(1848..1850)
                     /locus_tag="BspMI(1)"
                     /label=BspMI
                     /label=BspMI(1)
     misc_feature    complement(1848..1850)
                     /locus_tag="BspMI(4)"
                     /label=BspMI
                     /label=BspMI(4)
     misc_feature    complement(1852..2388)
                     /locus_tag="pro-CDS-Ter of SpecR"
                     /label=pro-CDS-Ter of SpecR
     misc_feature    complement(1852..2207)
                     /locus_tag="Spec gene"
                     /label=Spec gene
     misc_feature    complement(1852..2207)
                     /locus_tag="SpecR(1)"
                     /label=SpecR
                     /label=SpecR(1)
     misc_feature    complement(1852..1853)
                     /locus_tag="BspMI"
                     /label=BspMI
     misc_feature    complement(1852..1853)
                     /locus_tag="BspMI(5)"
                     /label=BspMI
                     /label=BspMI(5)
     misc_feature    1866..1884
                     /locus_tag="SpecR R"
                     /label=SpecR R
     misc_feature    complement(1883..1902)
                     /locus_tag="SpecR F"
                     /label=SpecR F
     misc_feature    1968
                     /locus_tag="Mut G->A, silent"
                     /label=Mut G->A, silent
     misc_feature    complement(1973..1978)
                     /locus_tag="BpmI"
                     /label=BpmI
     promoter        complement(2208..2355)
                     /locus_tag="specR_promoter_from_pKD13"
                     /label=specR_promoter_from_pKD13
     misc_feature    2242
                     /locus_tag="Mut C->A"
                     /label=Mut C->A
     misc_feature    complement(2370..2372)
                     /locus_tag="BspMI(3)"
                     /label=BspMI
                     /label=BspMI(3)
     misc_feature    complement(2374..2375)
                     /locus_tag="BspMI(2)"
                     /label=BspMI
                     /label=BspMI(2)
     misc_feature    complement(2561..2602)
                     /locus_tag="In T-DNA region AH003392.2"
                     /label=In T-DNA region AH003392.2
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctccgg agaggtccac
       61 cgcgccaatt gaaacaattg taaatttgca tggtgatgaa tttttagcag ccatcgaatt
      121 tgtagttagc atcactggtg attttctctt catattagga ttgagactga caggacaagt
      181 gacatccggt aattgtattt gcggagccaa cacttttccg tccactcctc ccgaagcgaa
      241 cccgtaccct gttaccctga ctctaaatgt aaattcatgt tgttggtacg gggtacgcct
      301 acttgtaagc tacggtatcc agttgggtgt gtagtgctcg tagtcattgt ctgtgcacat
      361 ttcaagtggt ccgtagcggg gccgggccag gccagcacga caaaaaagac cgtttggccg
      421 ccaagccgtg cgcggtccag cgcagtctag cactagagga gtaacgaaaa ataccattct
      481 catcccggca cgaacgcgag ggaacaacac aatcctgttt ttaccaaagt ccttttcccg
      541 aaacgaaaca caggttcgct ctgtaacacc agtaactctg cttacaagtt caccaatgcg
      601 agaccaccag gatacataga ttaccacaac tccgagccct tccacccaca gaatcagggg
      661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1141 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1381 gttaagggat tttggtcatg cattctaggt gattattatt tgccgactac cttggtgatc
     1441 tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     1501 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
     1561 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
     1621 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     1681 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     1741 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     1801 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc agcaagaatg
     1861 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
     1921 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcact ctctccaggg
     1981 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
     2041 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
     2101 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
     2161 acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgcg aaacgatcct
     2221 catcctgtct cttgatcaga tattgatccc ctgcgccatc agatccttgg cggcaagaaa
     2281 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
     2341 tccggttcgc ttgctaagct tgcatgcctg caagtcgact ctagaggagc ggtgatcaca
     2401 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
     2461 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
     2521 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
     2581 agtggtgatt ttgtgccgag ct
//
