LOCUS       Exported                2567 bp ds-DNA     circular UNA 19-MAY-2020
DEFINITION  backbone: pL0lacZ; Pbt promoter with AC overhangs for uLoop  .
ACCESSION   urn.local...pr-9jm71fk
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 20, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..40
                     /locus_tag="UNS1"
                     /label=UNS1
     misc_feature    complement(42..47)
                     /locus_tag="BsaI"
                     /label=BsaI
     misc_feature    46..566
                     /label=Phatr3_J54958_prohibitin PCR Product (concatena
                     /label=Phatr3_J54958_prohibitin PCR Product 
                     (concatenatedsequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:hh-ag1bzc4"
     misc_feature    46..48
                     /label=Ligation
                     /label=nonstandard type:  ligation
     terminator      53..559
                     /label=Pbt terminator  (Phatr3_J54958)
                     
                     /note="/created_by=134729"
                     /note="/modified_by=134729"
                     /note="/modified_by=13364144"
     misc_feature    join(564..2567,1..48)
                     /label=pL0R_lacZ (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: urn:local:.:hg-ag1bzc4"
     misc_feature    564..566
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    565..570
                     /locus_tag="BsaI(1)"
                     /label=BsaI
                     /label=BsaI(1)
     misc_feature    572..611
                     /locus_tag="UNSX"
                     /label=UNSX
     misc_feature    612..641
                     /locus_tag="Extra region of pBR322"
                     /label=Extra region of pBR322
     rep_origin      complement(702..1290)
                     /direction=LEFT
                     /locus_tag="pMB1-pUC19 ORI variant"
                     /label=pMB1-pUC19 ORI variant
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     misc_feature    complement(1381..1815)
                     /locus_tag="SpecR"
                     /label=SpecR
     misc_feature    complement(1381..1815)
                     /locus_tag="Spec gene(1)"
                     /label=Spec gene
                     /label=Spec gene(1)
     misc_feature    complement(1381..1815)
                     /locus_tag="pro-CDS-Ter of SpecR(1)"
                     /label=pro-CDS-Ter of SpecR
                     /label=pro-CDS-Ter of SpecR(1)
     misc_feature    complement(1813..1815)
                     /locus_tag="BspMI(1)"
                     /label=BspMI
                     /label=BspMI(1)
     misc_feature    complement(1813..1815)
                     /locus_tag="BspMI(4)"
                     /label=BspMI
                     /label=BspMI(4)
     misc_feature    complement(1817..2353)
                     /locus_tag="pro-CDS-Ter of SpecR"
                     /label=pro-CDS-Ter of SpecR
     misc_feature    complement(1817..2172)
                     /locus_tag="Spec gene"
                     /label=Spec gene
     misc_feature    complement(1817..2172)
                     /locus_tag="SpecR(1)"
                     /label=SpecR
                     /label=SpecR(1)
     misc_feature    complement(1817..1818)
                     /locus_tag="BspMI"
                     /label=BspMI
     misc_feature    complement(1817..1818)
                     /locus_tag="BspMI(5)"
                     /label=BspMI
                     /label=BspMI(5)
     misc_feature    1831..1849
                     /locus_tag="SpecR R"
                     /label=SpecR R
     misc_feature    complement(1848..1867)
                     /locus_tag="SpecR F"
                     /label=SpecR F
     misc_feature    1933
                     /locus_tag="Mut G->A, silent"
                     /label=Mut G->A, silent
     misc_feature    complement(1938..1943)
                     /locus_tag="BpmI"
                     /label=BpmI
     promoter        complement(2173..2320)
                     /locus_tag="specR_promoter_from_pKD13"
                     /label=specR_promoter_from_pKD13
     misc_feature    2207
                     /locus_tag="Mut C->A"
                     /label=Mut C->A
     misc_feature    complement(2335..2337)
                     /locus_tag="BspMI(3)"
                     /label=BspMI
                     /label=BspMI(3)
     misc_feature    complement(2339..2340)
                     /locus_tag="BspMI(2)"
                     /label=BspMI
                     /label=BspMI(2)
     misc_feature    complement(2526..2567)
                     /locus_tag="In T-DNA region AH003392.2"
                     /label=In T-DNA region AH003392.2
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctccgc ttagatagca
       61 gaagctggtc aaacttccaa atgacgaaat ctatgagcgt taataatata aatgtgagtg
      121 ccgacgattt catcaccaat tatctgcaag gatagaaagg gaagaatgaa aagagctgat
      181 tggtagattg cgctgaatgg gttgtttacg gtcttttttc tgaacacata cgcttcacaa
      241 attgattttc atgtctttcc ctctcccata ttctaaagaa ctgggaaggg ataagcttta
      301 cgtgctatgt catgacttct tagaataggt agctcaatcg gcatgtcctc attgtatatc
      361 agatgctgtc ttcttaggcg gcctcagttt ctaactctgt agagcagact ctttattgta
      421 cctacctgct aaatagaaga aagatcggat ttgcgtcttc ggcaaagcat cactcgtcaa
      481 tacgatccga cctcgtttcg aacacagcgt ggtgtagctg atggtgtgcc caccgacgct
      541 aactgtcaac atggcatcgc gctcgagacc accaggatac atagattacc acaactccga
      601 gcccttccac ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta
     1381 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
     1441 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     1501 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     1561 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     1621 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     1681 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     1741 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     1801 tggctcgaag ataccagcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     1861 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     1921 gcggagaatc tcactctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     1981 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     2041 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     2101 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     2161 cgcttccctc atgcgaaacg atcctcatcc tgtctcttga tcagatattg atcccctgcg
     2221 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     2281 accagagggc gccccagctg gcaattccgg ttcgcttgct aagcttgcat gcctgcaagt
     2341 cgactctaga ggagcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     2401 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     2461 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     2521 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagct
//
