LOCUS       pAKW.114A               3416 bp ds-DNA     circular     20-MAY-2020
DEFINITION  p15A_pCin_SarJ_PKA_L3S2P11.
ACCESSION   urn.local...1513168116087.84 (modified)
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     terminator      1..>56
                     /label="L3S3P21"
     misc_feature    53..55
                     /note="Geneious type: Editing History Insertion"
                     /label
     misc_feature    57
                     /note="Geneious type: Editing History Insertion"
                     /label
     promoter        77..302
                     /label="pCin"
     misc_feature    229..249
                     /note="Geneious type: operator"
                     /label="CinO"
     misc_feature    303..381
                     /note="Geneious type: insulator"
                     /label="SarJ"
     RBS             382..405
                     /label="Synthetic RBS (score: 499.89)"
     CDS             406..1458
                     /label="PKA"
     misc_feature    1460
                     /note="Geneious type: Editing History Replacement"
                     /label="G"
     terminator      1471..1527
                     /note="Geneious type: Terminator"
                     /label="L3S2P11"
     misc_feature    1526
                     /note="Geneious type: Editing History Replacement"
                     /label="C"
     misc_feature    1532
                     /note="Geneious type: Editing History Replacement"
                     /label="G"
     rep_origin      1593..2429
                     /label="more\stringent\p15A"
     misc_feature    complement(1600..1622)
                     /note="Geneious type: Primer"
                     /label="3' sequencing primer"
     CDS             complement(2501..3316)
                     /label="Kanamycin\phosphotransferase"
     misc_feature    3382..3412
                     /note="Geneious type: Primer"
                     /label="5' sequencing primer"
ORIGIN      
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct ccgtgctgct
       61 taacgatcgt tggctgccct ttgtgcgtcc aaacggacgc acggcgctct aaagcgggtc
      121 gcgatctttc agattcgctc ctcgcgcttt cagtctttgt tttggcgcat gtcgttatcg
      181 caaaaccgct gcacactttt gcgcgacatg ctctgatccc cctcatctgg gggggcctat
      241 ctgagggaat ttccgatccg gctcgcctga accattctgc tttccacgaa cttgaaaacg
      301 ctagactgtc gccggatgtg tatccgacct gacgatggcc caaaagggcc gaaacagtcc
      361 tctacaaata attttgttta acacaacaaa agccactaat tatttatggg taacgctgcc
      421 gccgccaaga agggcagcga gcaggagagc gtgaaagagt tcctagccaa agccaaggaa
      481 gatttcctga aaaaatggga aaccccttct cagaatacag cccagttgga tcagtttgat
      541 agaatcaaga cccttggcac cggctccttt gggcgagtga tgctggtgaa gcacaaggag
      601 agtgggaacc actacgccat gaagatctta gacaagcaga aggtggtgaa gctaaagcag
      661 atcgagcaca ctctgaatga gaagcgcatc ctgcaggccg tcaacttccc gttcctggtc
      721 aaacttgaat tctccttcaa ggacaactca aacctgtaca tggtcatgga gtatgtagct
      781 ggtggcgaga tgttctccca cctacggcgg attggaaggt tcagcgagcc ccatgcccgt
      841 ttctacgcgg cgcagatcgt cctgaccttt gagtatctgc actccctgga cctcatctac
      901 cgggacctga agcccgagaa tcttctcatc gaccagcagg gctatattca ggtgacagac
      961 ttcggttttg ccaagcgtgt gaaaggccgt acttggacct tgtgtgggac ccctgagtac
     1021 ttggcccccg agattatcct gagcaaaggc tacaacaagg ctgtggactg gtgggctctc
     1081 ggagtcctca tctacgagat ggctgctggt tacccaccct tcttcgctga ccagcctatc
     1141 cagatctatg agaaaatcgt ctctgggaag gtgcggttcc catcccactt cagctctgac
     1201 ttgaaggacc tgctgcggaa ccttctgcaa gtggatctca ccaagcgctt tgggaacctc
     1261 aagaacgggg tcaatgacat caagaaccac aagtggtttg ccacgactga ctggattgcc
     1321 atctatcaga gaaaggtgga agctcccttc ataccaaagt ttaaaggccc tggggacacg
     1381 agtaactttg acgactatga ggaggaagag atccgggttt ccatcaatga gaagtgtggc
     1441 aaggagttta ctgagtttta ataatactag ctcggtacca aattccagaa aagagacgct
     1501 ttcgagcgtc ttttttcgtt ttggtaccaa gtgagacccg gcgcgccatc gaatgaggtg
     1561 cttcctcgct cactgactcg ctgcacgagg cagacctcag cgctagcgga gtgtatactg
     1621 gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa
     1681 aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca
     1741 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
     1801 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
     1861 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
     1921 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
     1981 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     2041 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     2101 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     2161 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     2221 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     2281 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     2341 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     2401 aaacgatctc aagaagatca tcttattaag gggtctgacg ctcagtggaa cgaaaaatca
     2461 atctaaagta tatatgagta aacttggtct gacagttacc ttagaaaaac tcatcgagca
     2521 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     2581 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     2641 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     2701 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     2761 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     2821 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     2881 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     2941 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     3001 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     3061 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     3121 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     3181 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     3241 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc
     3301 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     3361 ttcatgatga tatattttta tcttgtgcaa tgtacatcag agattttgag acacaa
//
