LOCUS       EC10161_pL1V-loxPrev    3103 bp ds-DNA     circular     23-JAN-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_recomb     complement(1083..1133)
                     /label="loxP\rev"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    428..431
                     /label="TACT\Fusion\Site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(2178..2975)
                     /label="KanR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(514..1050)
                     /label="lacZ"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    483..486
                     /label="AATG\Fusion\Site"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1079..1082
                     /label="CGCT\Fusion\Site"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_recomb     complement(432..482)
                     /label="loxP\rev"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      1363..2030
                     /label="Col\E1\origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggcctaggc gcgccatgag ctctaaagct tcgctcgagc tgcggccgcc acttcgtgca
      421 cgtctcatac tgacctaata acttcgtata gcatacatta tacgaagtta tattaagggt
      481 tgaatgtgag accgtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
      541 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
      601 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
      661 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
      721 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
      781 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga
      841 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat
      901 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      961 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1021 cgttgcgctc actgcccgct ttccagtcgg caaacctgtc gtgccagctg cggtctcacg
     1081 ctgacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg ttgaatgtga
     1141 gacgcacaga gtggcggccg cttggatccc agaattctag gtacctctta attaactggc
     1201 ctcatgggcc ttcctttcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1261 ttaacatggt catagctgtt tccttgcgta ttgggcgctc tccgcttcct cgctcactga
     1321 ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa
     1381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2161 taaacttggt ctgacagtta ttagaaaaat tcatccagca gacgataaaa cgcaatacgc
     2221 tggctatccg gtgccgcaat gccatacagc accagaaaac gatccgccca ttcgccgccc
     2281 agttcttccg caatatcacg ggtggccagc gcaatatcct gataacgatc cgccacgccc
     2341 agacggccgc aatcaataaa gccgctaaaa cggccatttt ccaccataat gttcggcagg
     2401 cacgcatcac catgggtcac caccagatct tcgccatccg gcatgctcgc tttcagacgc
     2461 gcaaacagct ctgccggtgc caggccctga tgttcttcat ccagatcatc ctgatccacc
     2521 aggcccgctt ccatacgggt acgcgcacgt tcaatacgat gtttcgcctg atgatcaaac
     2581 ggacaggtcg ccgggtccag ggtatgcaga cgacgcatgg catccgccat aatgctcact
     2641 ttttctgccg gcgccagatg gctagacagc agatcctgac ccggcacttc gcccagcagc
     2701 agccaatcac ggcccgcttc ggtcaccaca tccagcaccg ccgcacacgg aacaccggtg
     2761 gtggccagcc agctcagacg cgccgcttca tcctgcagct cgttcagcgc accgctcaga
     2821 tcggttttca caaacagcac cggacgaccc tgcgcgctca gacgaaacac cgccgcatca
     2881 gagcagccaa tggtctgctg cgcccaatca tagccaaaca gacgttccac ccacgctgcc
     2941 gggctacccg catgcaggcc atcctgttca atcatactct tcctttttca atattattga
     3001 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3061 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//