LOCUS       pBS1479_HTP_KanM        4825 bp ds-DNA     circular     22-MAY-2020
SOURCE      
  ORGANISM  
COMMENT     http://www.invitrogen.com/
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2261..2281
                     /vntifkey="21"
                     /note="TEV cleavage site"
                     /locus_tag="TEV cleavage site"
                     /ApEinfo_label="TEV cleavage site"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2234..2260
                     /vntifkey="21"
                     /locus_tag="Spacer\1"
                     /ApEinfo_label="Spacer\1"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2288..2698
                     /vntifkey="21"
                     /note="Protein A Tag"
                     /locus_tag="Protein A\tags"
                     /ApEinfo_label="Protein A\tags"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2282..2287
                     /vntifkey="21"
                     /note="Spacer 2"
                     /locus_tag="Spacer\2"
                     /ApEinfo_label="Spacer\2"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     exon            129..986
                     /vntifkey="61"
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    2216..2233
                     /vntifkey="20"
                     /locus_tag="HIS6\tag"
                     /ApEinfo_label="HIS6\tag"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1266..1733
                     /vntifkey="33"
                     /locus_tag="pUC19 ORI"
                     /ApEinfo_label="pUC19 ORI"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3783..4126)
                     /locus_tag="TEF promotor"
                     /ApEinfo_label="TEF promotor"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2973..3782)
                     /locus_tag="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#00ff01"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2740..2972)
                     /locus_tag="TEF terminator"
                     /ApEinfo_label="TEF terminator"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2216..2239)
                     /locus_tag="part of L4"
                     /ApEinfo_label="part of L4"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4148..4168
                     /locus_tag="part of L5"
                     /ApEinfo_label="part of L5"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggaggatcca tggagcacca
     2221 tcaccatcac catgattatg atattccaac tactgctagc gagaatttgt attttcaggg
     2281 tgagctcaaa accgcggctc ttgcgcaaca cgatgaagcc gtgGACAACA AATTCAACAA
     2341 AGAACAACAA AACGCGTTCT ATGAGATCTT ACATTTACCT AACTTAAACG AAGAACAACG
     2401 AAACGCCTTC ATCCAAAGTT TAAAAGATGA CCCAAGCCAA AGCGCTAACC TTTTAGCAGA
     2461 AGCTAAAAAG CTAAATGATG CTCAGGCGCC GAAAgtaGAC AACAAATTCA ACAAAGAACA
     2521 ACAAAACGCG TTCTATGAGA TCTTACATTT ACCTAACTTA AACGAAGAAC AACGAAACGC
     2581 CTTCATCCAA AGTTTAAAAG ATGACCCAAG CCAAAGCGCT AACCTTTTAG CAGAAGCTAA
     2641 AAAGCTAAAT GGTGCTCAGG CGCCGAAAgt agacgcgaat tccgcgggga agtcaacctg
     2701 aagcttgata tccatcgatg aattcgagct cgtttaaact ggatggcggc gttagtatcg
     2761 aatcgacagc agtatagcga ccagcattca catacgattg acgcatgata ttactttctg
     2821 cgcacttaac ttcgcatctg ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct
     2881 aacatttgat taaaatagaa caactacaat ataaaaaaac tatacaaatg acaagttctt
     2941 gaaaacaaga atctttttat tgtcagtact gattagaaaa actcatcgag catcaaatga
     3001 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     3061 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     3121 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     3181 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     3241 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     3301 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     3361 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     3421 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttg
     3481 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     3541 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     3601 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     3661 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     3721 aaatcagcat ccatgttgga atttaatcgc ggcctcgaaa cgtgagtctt ttccttaccc
     3781 atggttgttt atgttcggat gtgatgtgag aactgtatcc tagcaagatt ttaaaaggaa
     3841 gtatatgaaa gaagaacctc agtggcaaat cctaaccttt tatatttctc tacaggggcg
     3901 cggcgtgggg acaattcaac gcgtctgtga ggggagcgtt tccctgctcg caggtctgca
     3961 gcgaggagcc gtaatttttg cttcgcgccg tgcggccatc aaaatgtatg gatgcaaatg
     4021 attatacatg gggatgtatg ggctaaatgt acgggcgaca gtcacatcat gcccctgagc
     4081 tgcgcacgtc aagactgtca aggagggtat tctgggcctc catgtcgctg gccgggtgac
     4141 ccggcgggga cgaggcaagc taaacagggt acccaattcg ccctatagtg agtcgtatta
     4201 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4261 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4321 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     4381 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4441 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4501 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4561 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4621 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4681 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4741 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4801 caaaatatta acgcttacaa tttag   
//
