LOCUS       pRibo_Tt                8188 bp ds-DNA     circular     11-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Alexander S. Mankin
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 17, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        9..148
                     /locus_tag="pL"
                     /label="pL"
                     /ApEinfo_label="pL"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -35_signal      107..112
                     /locus_tag="-35"
                     /label="-35"
                     /ApEinfo_label="-35"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -10_signal      130..135
                     /locus_tag="-10"
                     /label="-10"
                     /ApEinfo_label="-10"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     precursor_RNA   181..319
                     /locus_tag="16S 5' precursor"
                     /label="16S 5' precursor"
                     /ApEinfo_label="16S 5' precursor"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rRNA            320..1768
                     /locus_tag="16S rRNA (5')"
                     /label="16S rRNA (5')"
                     /ApEinfo_label="16S rRNA (5')"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rRNA            1767..1781
                     /locus_tag="Linker 1"
                     /label="Linker 1"
                     /ApEinfo_label="Linker 1"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rRNA            1782..4685
                     /locus_tag="cp23S"
                     /label="cp23S"
                     /ApEinfo_label="cp23S"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rRNA            1826..1829
                     /locus_tag="cp linker (GAGA)"
                     /label="cp linker (GAGA)"
                     /ApEinfo_label="cp linker (GAGA)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3887..3887
                     /locus_tag="A2058G, Ery resistance"
                     /label="A2058G, Ery resistance"
                     /ApEinfo_label="A2058G, Ery resistance"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rRNA            4686..4695
                     /locus_tag="Linker 2"
                     /label="Linker 2"
                     /ApEinfo_label="Linker 2"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rRNA            4696..4784
                     /locus_tag="16S rRNA (3')"
                     /label="16S rRNA (3')"
                     /ApEinfo_label="16S rRNA (3')"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             4776..4784
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
                     /locus_tag="Shine-Dalgarno sequence"
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_label="Shine-Dalgarno sequence"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     precursor_RNA   4785..4841
                     /locus_tag="16S 3' precursor"
                     /label="16S 3' precursor"
                     /ApEinfo_label="16S 3' precursor"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     tRNA            4956..5031
                     /locus_tag="Glu-tRNA"
                     /label="Glu-tRNA"
                     /ApEinfo_label="Glu-tRNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rRNA            5328..5446
                     /locus_tag="5S rRNA"
                     /label="5S rRNA"
                     /ApEinfo_label="5S rRNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      5447..5533
                     /gene="Escherichia coli rrnB"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /locus_tag="rrnB T1 terminator"
                     /label="rrnB T1 terminator"
                     /ApEinfo_label="rrnB T1 terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      5625..5652
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
                     /locus_tag="rrnB T2 terminator"
                     /label="rrnB T2 terminator"
                     /ApEinfo_label="rrnB T2 terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        5735..5763
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
                     /locus_tag="tac promoter"
                     /label="tac promoter"
                     /ApEinfo_label="tac promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     tRNA            5837..5913
                     /locus_tag="tRNA Asp"
                     /label="tRNA Asp"
                     /ApEinfo_label="tRNA Asp"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     tRNA            5922..5997
                     /locus_tag="tRNA Trp"
                     /label="tRNA Trp"
                     /ApEinfo_label="tRNA Trp"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     tRNA            6036..6112
                     /locus_tag="tRNA Ile"
                     /label="tRNA Ile"
                     /ApEinfo_label="tRNA Ile"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     tRNA            6155..6230
                     /locus_tag="tRNA Ala"
                     /label="tRNA Ala"
                     /ApEinfo_label="tRNA Ala"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     tRNA            6269..6344
                     /locus_tag="tRNA Glu"
                     /label="tRNA Glu"
                     /ApEinfo_label="tRNA Glu"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      6361..6403
                     /locus_tag="T1"
                     /label="T1"
                     /ApEinfo_label="T1"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        6420..6524
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             6525..7385
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     I
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     S
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     W
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     A
                     LPASWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     S LIKHW"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      7555..8143
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gcggccgcga tctctcacct accaaacaat gcccccctgc aaaaaataaa ttcatataaa
       61 aaacatacag ataaccatct gcggtgataa attatctctg gcggtgttga cataaatacc
      121 actggcggtg atactgagca cgggtaccgg ccgctgagaa aaagcgaagc ggcactgctc
      181 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
      241 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg aagtttaatt
      301 ctttgagcgt caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
      361 gcaggcctaa cacatgcaag tcgaacggta acaggaagaa gcttgcttct ttgctgacga
      421 gtggcggacg ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa
      481 acggtagcta ataccgcata acgtcgcaag accaaagagg gggaccttcg ggcctcttgc
      541 catcggatgt gcccagatgg gattagctag taggtggggt aacggctcac ctaggcgacg
      601 atccctagct ggtctgagag gatgaccagc cacactggaa ctgagacacg gtccagactc
      661 ctacgggagg cagcagtggg gaatattgca caatgggcgc aagcctgatg cagccatgcc
      721 gcgtgtatga agaaggcctt cgggttgtaa agtactttca gcggggagga agggagtaaa
      781 gttaatacct ttgctcattg acgttacccg cagaagaagc accggctaac tccgtgccag
      841 cagccgcggt aatacggagg gtgcaagcgt taatcggaat tactgggcgt aaagcgcacg
      901 caggcggttt gttaagtcag atgtgaaatc cccgggctca acctgggaac tgcatctgat
      961 actggcaagc ttgagtctcg tagagggggg tagaattcca ggtgtagcgg tgaaatgcgt
     1021 agagatctgg aggaataccg gtggcgaagg cggccccctg gacgaagact gacgctcagg
     1081 tgcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg
     1141 tcgacttgga ggttgtgccc ttgaggcgtg gcttccggag ctaacgcgtt aagtcgaccg
     1201 cctggggagt acggccgcaa ggttaaaact caaatgaatt gacgggggcc cgcacaagcg
     1261 gtggagcatg tggtttaatt cgatgcaacg cgaagaacct tacctggtct tgacatccac
     1321 ggaagttttc agagatgaga atgtgccttc gggaaccgtg agacaggtgc tgcatggctg
     1381 tcgtcagctc gtgttgtgaa atgttgggtt aagtcccgca acgagcgcaa cccttatcct
     1441 ttgttgccag cggtccggcc gggaactcaa aggagactgc cagtgataaa ctggaggaag
     1501 gtggggatga cgtcaagtca tcatggccct tacgaccagg gctacacacg tgctacaatg
     1561 gcgcatacaa agagaagcga cctcgcgaga gcaagcggac ctcataaagt gcgtcgtagt
     1621 ccggattgga gtctgcaact cgactccatg aagtcggaat cgctagtaat cgtggatcag
     1681 aatgccacgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac catgggagtg
     1741 ggttgcaaaa gaagtaggta gcttaaccca atgaacaatt ggatgcgttg agctaaccgg
     1801 tactaatgaa ccgtgaggct taaccgagag gttaagcgac taagcgtaca cggtggatgc
     1861 cctggcagtc agaggcgatg aaggacgtgc taatctgcga taagcgtcgg taaggtgata
     1921 tgaaccgtta taaccggcga tttccgaatg gggaaaccca gtgtgtttcg acacactatc
     1981 attaactgaa tccataggtt aatgaggcga accgggggaa ctgaaacatc taagtacccc
     2041 gaggaaaaga aatcaaccga gattccccca gtagcggcga gcgaacgggg agcagcccag
     2101 agcctgaatc agtgtgtgtg ttagtggaag cgtctggaaa ggcgcgcgat acagggtgac
     2161 agccccgtac acaaaaatgc acatgctgtg agctcgatga gtagggcggg acacgtggta
     2221 tcctgtctga atatgggggg accatcctcc aaggctaaat actcctgact gaccgatagt
     2281 gaaccagtac cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct
     2341 gaaaccgtgt acgtacaagc agtgggagca cgcttaggcg tgtgactgcg taccttttgt
     2401 ataatgggtc agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga
     2461 aaccgagtct taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc
     2521 catgggcagg ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa
     2581 aattagcgga tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt
     2641 ctccccgaaa gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt
     2701 tcggcaaggg ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt
     2761 tatcacggga gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac
     2821 cgccagctaa ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag
     2881 ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga
     2941 gtcggcctgc gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg
     3001 cttatgcgtt gttgggtagg ggagcgttct gtaagcctgc gaaggtgtgc tgtgaggcat
     3061 gctggaggta tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc
     3121 gctcgccgga agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc
     3181 taaggcgagg ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt
     3241 tactgcgaag gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag
     3301 cgtgtaggct ggttttccag gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc
     3361 actacggtgc tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa
     3421 catcaaatcg taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag
     3481 agaactcggg tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga
     3541 tatgtaggtg aggtccctcg cggatggagc tgaaatcagt cgaagatacc agctggctgc
     3601 aactgtttat taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg
     3661 cctgcccggt gccggaaggt taattgatgg ggttagcgca agcgaagctc ttgatcgaag
     3721 ccccggtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt
     3781 aagttccgac ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt
     3841 gaaattgaac tcgctgtgaa gatgcagtgt acccgcggca agacgggaag accccgtgaa
     3901 cctttactat agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt
     3961 tgaagtgtgg acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga
     4021 tgttctaacg ttgacccgta atccgggttg cggacagtgt ctggtgggta gtttgactgg
     4081 ggcggtctcc tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca
     4141 tcaggaggtt agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg
     4201 tgcgaaagca ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat
     4261 aaaaggtact ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt
     4321 ttggcacctc gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc
     4381 tgttcgccat ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc
     4441 ctatctgccg tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag
     4501 tggacgcatc actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg
     4561 gaagagataa gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg
     4621 accctttaag ggtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa
     4681 gcgcagataa ctagtggagg gcgcttacca ctttgtgatt catgactggg gtgaagtcgt
     4741 aacaaggtaa ccgtagggga acctgcggtt ggatcacctc cttaccttaa agaagcgtac
     4801 tttgtagtgc tcacacagat tgtctgatag aaagtgaaaa gcaaggcgtt tacgcgttgg
     4861 gagtgaggct gaagagaata aggccgttcg ctttctatta atgaaagctc accctacacg
     4921 aaaatatcac gcaacgcgtg ataagcaatt ttcgtgtccc cttcgtctag aggcccagga
     4981 caccgccctt tcacggcggt aacaggggtt cgaatcccct aggggacgcc acttgctggt
     5041 ttgtgagtga aagtcgccga ccttaatatc tcaaaactca tcttcgggtg atgtttgaga
     5101 tatttgctct ttaaaaatct ggatcaagct gaaaattgaa acactgaaca acgagagttg
     5161 ttcgtgagtc tctcaaattt tcgcaacacg atgatgaatc gaaagaaaca tcttcgggtt
     5221 gtgagcttaa gcttacaacg ccgaagctgt tttggcggat gagagaagat tttcagcctg
     5281 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
     5341 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     5401 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     5461 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     5521 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     5581 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
     5641 ggatggcctt tttgcgtttc tacaaactct tcctgtcgtc atatctacaa gccggctttc
     5701 ctttccatca aaaaaatatt gatgaaatga gctgttgaca attaatcatc ggctcgtata
     5761 atgtgtggaa ttgtcacaca ggaaacagaa ttcccgggga tctgggggat catcgatggt
     5821 tgtaaaagaa ttcggtggag cggtagttca gtcggttaga atacctgcct gtcacgcagg
     5881 gggtcgcggg ttcgagtccc gtccgttccg ccaccctaat taggggcgta gttcaattgg
     5941 tagagcaccg gtctccaaaa ccgggtgttg ggagttcgag tctctccgcc cctgccagaa
     6001 atcatccttg tcgatgggag cagtaaaacc tctacaggct tgtagctcag gtggttagag
     6061 cgcacccctg ataagggtga ggtcggtggt tcaagtccac tcaggcctac caaatttgca
     6121 cggcaaattt gaagaggttt taactacatg ttatggggct atagctcagc tgggagagcg
     6181 cctgctttgc acgcaggagg tctgcggttc gatcccgcat agctccacca tctctgtagt
     6241 gattaagagc gtgataagca attttcgtgt ccccttcgtc tagaggccca ggacaccgcc
     6301 ctttcacggc ggtaacaggg gttcgaatcc cctaggggac gccactctag gaaatccgcc
     6361 ataaaacaaa aggctcagtc ggaagactgg gccttttgtt ttacgcgccg ggaaatgtgc
     6421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     6781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgcaataa
     6901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     7081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggcta
     7201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7381 ggtaactgca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7441 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7501 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7561 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7621 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7681 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     7741 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7801 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7861 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7921 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7981 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8041 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8101 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8161 ctttttacgg ttcctggcct tttgctgg   
//
