LOCUS       pEG01_(P_BAD-fucO-aldA- 5195 bp ds-DNA     circular     22-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature         4844..4864
                          /label="N31"
                          /ApEinfo_revcolor=#d6b295
                          /ApEinfo_fwdcolor=#d6b295
     terminator           4868..4995
                          /label="T7\terminator"
                          /ApEinfo_revcolor=#ff9ccd
                          /ApEinfo_fwdcolor=#ff9ccd
     misc_feature         3410..4843
                          /label="aldA"
                          /ApEinfo_revcolor=#f8d3a9
                          /ApEinfo_fwdcolor=#f8d3a9
     CDS                  54..846
                          /label="spect"
                          /ApEinfo_revcolor=#f8d3a9
                          /ApEinfo_fwdcolor=#f8d3a9
     misc_feature         3375..3409
                          /label="RBS2stop"
                          /ApEinfo_revcolor=#9eafd2
                          /ApEinfo_fwdcolor=#9eafd2
     misc_feature         1892..2188
                          /label="pBAD"
                          /ApEinfo_revcolor=#ff9ccd
                          /ApEinfo_fwdcolor=#ff9ccd
     CDS                  1037..1625
                          /label="pMB1"
                          /ApEinfo_revcolor=#b7e6d7
                          /ApEinfo_fwdcolor=#b7e6d7
     promoter_prokaryotic 1892..2164
                          /label="araBAD promoter and regulatory elements"
                          /ApEinfo_revcolor=#b7e6d7
                          /ApEinfo_fwdcolor=#b7e6d7
     misc_feature         966..984
                          /label="N22"
                          /ApEinfo_revcolor=#9eafd2
                          /ApEinfo_fwdcolor=#9eafd2
     primer               2096..2115
                          /label="pBAD forward primer"
                          /ApEinfo_revcolor=#75c6a9
                          /ApEinfo_fwdcolor=#75c6a9
     misc_feature         5065..5083
                          /label="N21"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     misc_feature         2232..3374
                          /label="fucO"
                          /ApEinfo_revcolor=#d6b295
                          /ApEinfo_fwdcolor=#d6b295
     misc_feature         1873..1891
                          /label="N23"
                          /ApEinfo_revcolor=#f8d3a9
                          /ApEinfo_fwdcolor=#f8d3a9
     misc_feature         2189..2231
                          /label="RBS1"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat gtcatgaggg
       61 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
      121 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
      181 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
      241 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
      301 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
      361 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
      421 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
      481 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
      541 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
      601 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
      661 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
      721 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
      781 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
      841 aataagatgc cgctcgccag tcgattggct gagctcatga agttcctatt ccgaagttcc
      901 gcgaacgcgt aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      961 taacgtcaca caacatagcc acggagtttt cgttccactg agcgtcagac cccgtagaaa
     1021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1741 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1801 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     1861 tatgctggat cctcaaacag aaaggccatg gaaaccaatt gtccatattg catcagacat
     1921 tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt
     1981 aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc
     2041 tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc
     2101 atagcatttt tatccataag attagcggat cctacctgac gctttttatc gcaactctct
     2161 actgtttctc catacccgtt ttttgggcta gaaataattt tgtttaactt taagaaggag
     2221 atatacatat ggctaacaga atgattctga acgaaacggc atggtttggt cggggtgctg
     2281 ttggggcttt aaccgatgag gtgaaacgcc gtggttatca gaaggcgctg atcgtcaccg
     2341 ataaaacgct ggtgcaatgc ggcgtggtgg cgaaagtgac cgataagatg gatgctgcag
     2401 ggctggcatg ggcgatttac gacggcgtag tgcccaaccc aacaattact gtcgtcaaag
     2461 aagggctcgg tgtattccag aatagcggcg cggattacct gatcgctatt ggtggtggtt
     2521 ctccacagga tacttgtaaa gcgattggca ttatcagcaa caacccggag tttgccgatg
     2581 tgcgtagcct ggaagggctt tccccgacca ataaacccag tgtaccgatt ctggcaattc
     2641 ctaccacagc aggtactgcg gcagaagtga ccattaacta cgtgatcact gacgaagaga
     2701 aacggcgcaa gtttgtttgc gttgatccgc atgatatccc gcaggtggcg tttattgacg
     2761 ctgacatgat ggatggtatg cctccagcgc tgaaagctgc gacgggtgtc gatgcgctca
     2821 ctcatgctat tgaggggtat attacccgtg gcgcgtgggc gctaaccgat gcactgcaca
     2881 ttaaagcgat tgaaatcatt gctggggcgc tgcgaggatc ggttgctggt gataaggatg
     2941 ccggagaaga aatggcgctc gggcagtatg ttgcgggtat gggcttctcg aatgttgggt
     3001 tagggttggt gcatggtatg gcgcatccac tgggcgcgtt ttataacact ccacacggtg
     3061 ttgcgaacgc catcctgtta ccgcatgtca tgcgttataa cgctgacttt accggtgaga
     3121 agtaccgcga tatcgcgcgc gttatgggcg tgaaagtgga aggtatgagc ctggaagagg
     3181 cgcgtaatgc cgctgttgaa gcggtgtttg ctctcaaccg tgatgtcggt attccgccac
     3241 atttgcgtga tgttggtgta cgcaaggaag acattccggc actggcgcag gcggcactgg
     3301 atgatgtttg taccggtggc aacccgcgtg aagcaacgct tgaggatatt gtagagcttt
     3361 accataccgc ctggTaaccg ttcatttatc acaaaaggat tgttcgatGT CAGTACCCGT
     3421 TCAACATCCT ATGTATATCG ATGGACAGTT TGTTACCTGG CGTGGAGACG CATGGATTGA
     3481 TGTGGTAAAC CCTGCTACAG AGGCTGTCAT TTCCCGCATA CCCGATGGTC AGGCCGAGGA
     3541 TGCCCGTAAG GCAATCGATG CAGCAGAACG TGCACAACCA GAATGGGAAG CGTTGCCTGC
     3601 TATTGAACGC GCCAGTTGGT TGCGCAAAAT CTCCGCCGGG ATCCGCGAAC GCGCCAGTGA
     3661 AATCAGTGCG CTGATTGTTG AAGAAGGGGG CAAGATCCAG CAGCTGGCTG AAGTCGAAGT
     3721 GGCTTTTACT GCCGACTATA TCGATTACAT GGCGGAGTGG GCACGGCGTT ACGAGGGCGA
     3781 GATTATTCAA AGCGATCGTC CAGGAGAAAA TATTCTTTTG TTTAAACGTG CGCTTGGTGT
     3841 GACTACCGGC ATTCTGCCGT GGAACTTCCC GTTCTTCCTC ATTGCCCGCA AAATGGCTCC
     3901 CGCTCTTTTG ACCGGTAATA CCATCGTCAT TAAACCTAGT GAATTTACGC CAAACAATGC
     3961 GATTGCATTC GCCAAAATCG TCGATGAAAT AGGCCTTCCG CGCGGCGTGT TTAACCTTGT
     4021 ACTGGGGCGT GGTGAAACCG TTGGGCAAGA ACTGGCGGGT AACCCAAAGG TCGCAATGGT
     4081 CAGTATGACA GGCAGCGTCT CTGCAGGTGA GAAGATCATG GCGACTGCGG CGAAAAACAT
     4141 CACCAAAGTG TGTCTGGAAT TGGGGGGTAA AGCACCAGCT ATCGTAATGG ACGATGCCGA
     4201 TCTTGAACTG GCAGTCAAAG CCATCGTTGA TTCACGCGTC ATTAATAGTG GGCAAGTGTG
     4261 TAACTGTGCA GAACGTGTTT ATGTACAGAA AGGCATTTAT GATCAGTTCG TCAATCGGCT
     4321 GGGTGAAGCG ATGCAGGCGG TTCAATTTGG TAACCCCGCT GAACGCAACG ACATTGCGAT
     4381 GGGGCCGTTG ATTAACGCCG CGGCGCTGGA AAGGGTCGAG CAAAAAGTGG CGCGCGCAGT
     4441 AGAAGAAGGG GCGAGAGTGG CGTTCGGTGG CAAAGCGGTA GAGGGGAAAG GATATTATTA
     4501 TCCGCCGACA TTGCTGCTGG ATGTTCGCCA GGAAATGTCG ATTATGCATG AGGAAACCTT
     4561 TGGCCCGGTG CTGCCAGTTG TCGCATTTGA CACGCTGGAA GATGCTATCT CAATGGCTAA
     4621 TGACAGTGAT TACGGCCTGA CCTCATCAAT CTATACCCAA AATCTGAACG TCGCGATGAA
     4681 AGCCATTAAA GGGCTGAAGT TTGGTGAAAC TTACATCAAC CGTGAAAACT TCGAAGCTAT
     4741 GCAAGGCTTC CACGCCGGAT GGCGTAAATC CGGTATTGGC GGCGCAGATG GTAAACATGG
     4801 CTTGCATGAA TATCTGCAGA CCCAGGTGGT TTATTTACAG TCTTgatggg ctgaagggtt
     4861 taaGggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     4921 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     4981 gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc
     5041 tacagcatcc agggtgacgg tgccTccctc gactcacact tgGtcgacct gcagaagctt
     5101 agatctatta ccctgttatc cctactcgag ttcatgtgca gctccatcag caaaagggga
     5161 tgataagttt atcaccaccg actatttgca acagt
//