LOCUS       Exported                5451 bp ds-DNA     circular SYN 21-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 21, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        67..95
                     /label=Amp prom
     CDS             335..994
                     /label=AmpR
     gene            2526..4223
                     /label=Firefly luciferase
     intron          4353..4368
                     /label=SV40 int
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccaa gcttcccggg
     2461 tagatctctc gaggtcgaca atacaatata aagcatataa acagcccagc ttggagcgag
     2521 tgaccatggg agctcgaatt ccagcttggc attccggtac tgttggtaaa atggaagacg
     2581 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
     2641 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
     2701 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
     2761 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     2821 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     2881 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     2941 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     3001 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     3061 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     3121 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
     3181 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     3241 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     3301 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     3361 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     3421 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     3481 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     3541 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     3601 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     3661 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     3721 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     3781 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     3841 cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     3901 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     3961 tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg
     4021 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     4081 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     4141 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     4201 agggcggaaa gtccaaattg taaaatgtaa ctgtattcag cgatgacgaa attcttagct
     4261 attgtaatac tctagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     4321 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     4381 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaacttat
     4441 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     4501 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactctcaa aagaagagaa
     4561 aggtagagac ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     4621 agtaatagaa ctcttgcttg ctttgctatt tacaaccaca aaggaaaaag ctgcactgct
     4681 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     4741 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     4801 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     4861 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     4921 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     4981 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5041 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5101 atgtatctta tcatgtctgg atccccagga agctcctctg tgtcctcata aaccctaacc
     5161 tcctctactt gagaggacat tccaatcata ggctgcccat ccaccctctg tgtcctcctg
     5221 ttaattaggt cacttaacaa aaaggaaatt gggtaggggt ttttcacaga ccgctttcta
     5281 aggggtaatt ttaaaatatc tgggaagtcc cttccactgc tgtgttccag aagtgttggt
     5341 aaacagccca caaatgtcaa cagcagaaac atacaagctg tcactttgca caaagggcct
     5401 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt a
//
