LOCUS       Exported                5672 bp ds-DNA     circular SYN 21-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 21, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        67..95
                     /label=Amp prom
     CDS             335..994
                     /label=AmpR
     misc_feature    2439..2479
                     /label=MCS
     promoter        2480..2707
                     /label=Minimal DmHsp70 Promoter
     gene            2747..4444
                     /label=Firefly luciferase
     intron          4574..4589
                     /label=SV40 int
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccaa gcttcccggg
     2461 cagatctctc gaggtcgacc gccggagtat aaatagaggc gcttcgtcta cggagcgaca
     2521 attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag
     2581 cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa
     2641 gtaaccagca accaagtaaa tcaactgcaa ctactgagat ctgccaagaa gtaattattg
     2701 aatacaatat aaagcatata aacagcccag cttggagcga gtgaccatgg gagctcgaat
     2761 tccagcttgg cattccggta ctgttggtaa aatggaagac gccaaaaaca taaagaaagg
     2821 cccggcgcca ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat
     2881 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa
     2941 catcacgtac gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     3001 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     3061 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     3121 tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa
     3181 aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc agaaaattat
     3241 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc
     3301 tcatctacct cccggtttta atgaatacga ttttgtacca gagtcctttg atcgtgacaa
     3361 aacaattgca ctgataatga attcctctgg atctactggg ttacctaagg gtgtggccct
     3421 tccgcataga actgcctgcg tcagattctc gcatgccaga gatcctattt ttggcaatca
     3481 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
     3541 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
     3601 agagctgttt ttacgatccc ttcaggatta caaaattcaa agtgcgttgc tagtaccaac
     3661 cctattttca ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca
     3721 cgaaattgct tctgggggcg cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg
     3781 cttccatctt ccagggatac gacaaggata tgggctcact gagactacat cagctattct
     3841 gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga
     3901 agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaga gaggcgaatt
     3961 atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc
     4021 cttgattgac aaggatggat ggctacattc tggagacata gcttactggg acgaagacga
     4081 acacttcttc atagttgacc gcttgaagtc tttaattaaa tacaaaggat atcaggtggc
     4141 ccccgctgaa ttggaatcga tattgttaca acaccccaac atcttcgacg cgggcgtggc
     4201 aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg
     4261 aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa
     4321 aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact
     4381 cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa agtccaaatt
     4441 gtaaaatgta actgtattca gcgatgacga aattcttagc tattgtaata ctctagagga
     4501 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     4561 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     4621 taattgtttg tgtattttag attccaacct atggaactta tgaatgggag cagtggtgga
     4681 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     4741 ctactgctga ctctcaacat tctactctca aaagaagaga aaggtagaga cccaaggact
     4801 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     4861 gctttgctat ttacaaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     4921 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     4981 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     5041 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     5101 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5161 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5221 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5281 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5341 gatccccagg aagctcctct gtgtcctcat aaaccctaac ctcctctact tgagaggaca
     5401 ttccaatcat aggctgccca tccaccctct gtgtcctcct gttaattagg tcacttaaca
     5461 aaaaggaaat tgggtagggg tttttcacag accgctttct aaggggtaat tttaaaatat
     5521 ctgggaagtc ccttccactg ctgtgttcca gaagtgttgg taaacagccc acaaatgtca
     5581 acagcagaaa catacaagct gtcactttgc acaaagggcc tcgtgatacg cctattttta
     5641 taggttaatg tcatgataat aatggtttct ta
//
