LOCUS       Exported                6114 bp ds-DNA     circular SYN 17-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 21, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        32..136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             137..997
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     oriT            complement(1168..1747)
                     /direction=LEFT
                     /label=Ori
     polyA_signal    2313..2447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2541..2673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        2724..2733
                     /label=AaRel1A NFkB site
     enhancer        2747..2756
                     /label=AaRel1A NFkB site
     enhancer        2770..2779
                     /label=AaRel1A NFkB site
     enhancer        2793..2802
                     /label=AaRel1A NFkB site
     enhancer        2820..2829
                     /label=AaRel1A NFkB site
     enhancer        2843..2852
                     /label=AaRel1A NFkB site
     enhancer        2866..2875
                     /label=AaRel1A NFkB site
     enhancer        2889..2898
                     /label=AaRel1A NFkB site
     promoter        2922..3149
                     /label=Minimal DmHsp70 Promoter
     gene            3189..4886
                     /label=Firefly luciferase
     polyA_signal    5652..5786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcagc catatcacat
     2461 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2521 aaatgaatgc aattgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2581 atagcatcac aaatttcaca aataaagcat ttttcactgc attctagttg tggtttgtcc
     2641 aaactcatca atgtatctta tcatgtctgg atccaagctt cccgggcaga tctctcgagg
     2701 tcgacccggg gatctcgaga cgcgggaaat tccaacgtcg agacgcggga aattccaacg
     2761 tcgagacgcg ggaaattcca acgtcgagac gcgggaaatt ccaacgaatt tcgagacgcg
     2821 ggaaattcca acgtcgagac gcgggaaatt ccaacgtcga gacgcgggaa attccaacgt
     2881 cgagacgcgg gaaattccaa cgagcttaat tagatctaat tcgccggagt ataaatagag
     2941 gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc
     3001 gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca gtaaagtgca
     3061 agttaaagtg aatcaattaa aagtaaccag caaccaagta aatcaactgc aactactgag
     3121 atctgccaag aagtaattat tgaatacaat ataaagcata taaacagccc agcttggagc
     3181 gagtgaccat gggagctcga attccagctt ggcattccgg tactgttggt aaaatggaag
     3241 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat ggaaccgctg
     3301 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
     3361 cagatgcaca tatcgaggtg aacatcacgt acgcggaata cttcgaaatg tccgttcggt
     3421 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
     3481 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
     3541 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgaac atttcgcagc
     3601 ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaaat
     3661 taccaataat ccagaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
     3721 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtac
     3781 cagagtcctt tgatcgtgac aaaacaattg cactgataat gaattcctct ggatctactg
     3841 ggttacctaa gggtgtggcc cttccgcata gaactgcctg cgtcagattc tcgcatgcca
     3901 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
     3961 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
     4021 tcttaatgta tagatttgaa gaagagctgt ttttacgatc ccttcaggat tacaaaattc
     4081 aaagtgcgtt gctagtacca accctatttt cattcttcgc caaaagcact ctgattgaca
     4141 aatacgattt atctaattta cacgaaattg cttctggggg cgcacctctt tcgaaagaag
     4201 tcggggaagc ggttgcaaaa cgcttccatc ttccagggat acgacaagga tatgggctca
     4261 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
     4321 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
     4381 gcgttaatca gagaggcgaa ttatgtgtca gaggacctat gattatgtcc ggttatgtaa
     4441 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
     4501 tagcttactg ggacgaagac gaacacttct tcatagttga ccgcttgaag tctttaatta
     4561 aatacaaagg atatcaggtg gcccccgctg aattggaatc gatattgtta caacacccca
     4621 acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
     4681 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
     4741 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
     4801 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
     4861 agaagggcgg aaagtccaaa ttgtaaaatg taactgtatt cagcgatgac gaaattctta
     4921 gctattgtaa tactctagag gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     4981 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     5041 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     5101 tatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     5161 atgccatcta gtgatgatga ggctactgct gactctcaac attctactct caaaagaaga
     5221 gaaaggtaga gacccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     5281 tttagtaata gaactcttgc ttgctttgct atttacaacc acaaaggaaa aagctgcact
     5341 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     5401 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     5461 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     5521 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     5581 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     5641 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5701 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5761 tcaatgtatc ttatcatgtc tggatcccca ggaagctcct ctgtgtcctc ataaacccta
     5821 acctcctcta cttgagagga cattccaatc ataggctgcc catccaccct ctgtgtcctc
     5881 ctgttaatta ggtcacttaa caaaaaggaa attgggtagg ggtttttcac agaccgcttt
     5941 ctaaggggta attttaaaat atctgggaag tcccttccac tgctgtgttc cagaagtgtt
     6001 ggtaaacagc ccacaaatgt caacagcaga aacatacaag ctgtcacttt gcacaaaggg
     6061 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt ctta
//
