LOCUS       Exported                5888 bp ds-DNA     circular SYN 21-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 21, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        67..95
                     /label=Amp prom
     CDS             335..994
                     /label=AmpR
     misc_feature    2665..2705
                     /label=MCS
     enhancer        2724..2733
                     /label=AaRel1A NFkB site
     enhancer        2747..2756
                     /label=AaRel1A NFkB site
     enhancer        2770..2779
                     /label=AaRel1A NFkB site
     enhancer        2793..2802
                     /label=AaRel1A NFkB site
     enhancer        2820..2829
                     /label=AaRel1A NFkB site
     enhancer        2843..2852
                     /label=AaRel1A NFkB site
     enhancer        2866..2875
                     /label=AaRel1A NFkB site
     enhancer        2889..2898
                     /label=AaRel1A NFkB site
     gene            2963..4660
                     /label=Firefly luciferase
     intron          4790..4805
                     /label=SV40 int
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcagc catatcacat
     2461 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2521 aaatgaatgc aattgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2581 atagcatcac aaatttcaca aataaagcat ttttcactgc attctagttg tggtttgtcc
     2641 aaactcatca atgtatctta tcatgtctgg atccaagctt cccgggcaga tctctcgagg
     2701 tcgacccggg gatctcgaga cgcgggaaat tccaacgtcg agacgcggga aattccaacg
     2761 tcgagacgcg ggaaattcca acgtcgagac gcgggaaatt ccaacgaatt tcgagacgcg
     2821 ggaaattcca acgtcgagac gcgggaaatt ccaacgtcga gacgcgggaa attccaacgt
     2881 cgagacgcgg gaaattccaa cgagcttaat tagatctaat taatataaag catataaaca
     2941 gcccagcttg gagcgagtga ccatgggagc tcgaattcca gcttggcatt ccggtactgt
     3001 tggtaaaatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atcctctaga
     3061 ggatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg
     3121 aacaattgct tttacagatg cacatatcga ggtgaacatc acgtacgcgg aatacttcga
     3181 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat
     3241 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat
     3301 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat
     3361 gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa
     3421 cgtgcaaaaa aaattaccaa taatccagaa aattattatc atggattcta aaacggatta
     3481 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga
     3541 atacgatttt gtaccagagt cctttgatcg tgacaaaaca attgcactga taatgaattc
     3601 ctctggatct actgggttac ctaagggtgt ggcccttccg catagaactg cctgcgtcag
     3661 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt
     3721 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg
     3781 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttttac gatcccttca
     3841 ggattacaaa attcaaagtg cgttgctagt accaacccta ttttcattct tcgccaaaag
     3901 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg ggggcgcacc
     3961 tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc catcttccag ggatacgaca
     4021 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa
     4081 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac
     4141 cgggaaaacg ctgggcgtta atcagagagg cgaattatgt gtcagaggac ctatgattat
     4201 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct
     4261 acattctgga gacatagctt actgggacga agacgaacac ttcttcatag ttgaccgctt
     4321 gaagtcttta attaaataca aaggatatca ggtggccccc gctgaattgg aatcgatatt
     4381 gttacaacac cccaacatct tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg
     4441 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat
     4501 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt
     4561 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat
     4621 cctcataaag gccaagaagg gcggaaagtc caaattgtaa aatgtaactg tattcagcga
     4681 tgacgaaatt cttagctatt gtaatactct agaggatctt tgtgaaggaa ccttacttct
     4741 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     4801 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     4861 caacctatgg aacttatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     4921 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     4981 ctctcaaaag aagagaaagg tagagaccca aggactttcc ttcagaattg ctaagttttt
     5041 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac aaccacaaag
     5101 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     5161 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     5221 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     5281 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     5341 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5401 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5521 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccaggaagc tcctctgtgt
     5581 cctcataaac cctaacctcc tctacttgag aggacattcc aatcataggc tgcccatcca
     5641 ccctctgtgt cctcctgtta attaggtcac ttaacaaaaa ggaaattggg taggggtttt
     5701 tcacagaccg ctttctaagg ggtaatttta aaatatctgg gaagtccctt ccactgctgt
     5761 gttccagaag tgttggtaaa cagcccacaa atgtcaacag cagaaacata caagctgtca
     5821 ctttgcacaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5881 gtttctta
//
