LOCUS       Exported                6046 bp ds-DNA     circular SYN 18-NOV-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    (P118) pKM59
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 21, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
COMMENT     Fragment extracted from (P61) pLUC-MCS [5592 nt] : (SacI[2735] / 
            HindIII[2675])
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    51..66
                     /note="Ecdysone"
     protein_bind    97..105
                     /note="Mtk Rel site"
     protein_bind    156..174
                     /note="Ecdysone"
     protein_bind    226..233
                     /note="Mtk GATA site"
     protein_bind    243..252
                     /note="Mtk Rel site"
     protein_bind    complement(271..278)
                     /note="Mtk Rel site"
     protein_bind    complement(291..298)
                     /note="Mtk GATA site"
     protein_bind    308..323
                     /note="Ecdysone"
     protein_bind    complement(335..342)
                     /note="Mtk GATA site"
     protein_bind    complement(357..365)
                     /note="Mtk Rel site"
     protein_bind    433..442
                     /note="Mtk Rel site new prediction"
     gene            457..2154
                     /label=Firefly luciferase
                     /note="Firefly luciferase"
     intron          2284..2299
                     /note="SV40 int"
     protein_bind    3214..3222
                     /note="Mtk Rel site"
     protein_bind    3233..3242
                     /note="Mtk Rel site"
     promoter        3449..3477
                     /note="Amp prom"
     CDS             3717..4376
                     /label=AmpR
                     /note="AmpR"
     protein_bind    4466..4473
                     /note="Mtk GATA site"
ORIGIN
        1 gtctggatcc aagcttgccc gggcagatct ctcgaggcgt ggctccgtgt tgacgcatgt
       61 tgactatgcc tttgaatggc tgccgtggtt gtcggtgggt aatttgcaat gcagaaaaac
      121 caacagggcg ctaaaaagga gagtgttttc gtgggaggtg gagatggtca ctgggggcaa
      181 cataaatatt cagcgagaaa cgtcatattt acatttagtc taggctgata atccgggacc
      241 gtgggaagtc ccctttgggt ggtgctggct gggttcccct ggccacaatc ggttatctgc
      301 ccccggctga cacttgcccg tcattcattc ggctgcttat cgcagaagct caaataaaaa
      361 gtccccaatc tgcgactcgt ttgtctggga ctgagctata aaagcctcac catctcaacg
      421 ctcaaagcat caatcaattc ccgccaccga gctaagatgg gagctcgaat tccagcttgg
      481 cattccggta ctgttggtaa aatggaagac gccaaaaaca taaagaaagg cccggcgcca
      541 ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
      601 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac
      661 gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
      721 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
      781 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
      841 ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg
      901 caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat
      961 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
     1021 cccggtttta atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca
     1081 ctgataatga attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga
     1141 actgcctgcg tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
     1201 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
     1261 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
     1321 ttacgatccc ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca
     1381 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     1441 tctgggggcg cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt
     1501 ccagggatac gacaaggata tgggctcact gagactacat cagctattct gattacaccc
     1561 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     1621 gtggatctgg ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga
     1681 ggacctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     1741 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc
     1801 atagttgacc gcttgaagtc tttaattaaa tacaaaggat atcaggtggc ccccgctgaa
     1861 ttggaatcga tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc
     1921 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     1981 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc
     2041 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     2101 aaaatcagag agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaaaatgta
     2161 actgtattca gcgatgacga aattcttagc tattgtaata ctctagagga tctttgtgaa
     2221 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     2281 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     2341 tgtattttag attccaacct atggaactta tgaatgggag cagtggtgga atgcctttaa
     2401 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     2461 ctctcaacat tctactctca aaagaagaga aaggtagaga cccaaggact ttccttcaga
     2521 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     2581 ttacaaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     2641 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     2701 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     2761 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     2821 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     2881 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     2941 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3001 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccagg
     3061 aagctcctct gtgtcctcat aaaccctaac ctcctctact tgagaggaca ttccaatcat
     3121 aggctgccca tccaccctct gtgtcctcct gttaattagg tcacttaaca aaaaggaaat
     3181 tgggtagggg tttttcacag accgctttct aaggggtaat tttaaaatat ctgggaagtc
     3241 ccttccactg ctgtgttcca gaagtgttgg taaacagccc acaaatgtca acagcagaaa
     3301 catacaagct gtcactttgc acaaagggcc tcgtgatacg cctattttta taggttaatg
     3361 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3421 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3481 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3541 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3601 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3661 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3721 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3781 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3841 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3901 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3961 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4021 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4081 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4141 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4201 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4261 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4321 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4381 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4441 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4501 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4561 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4621 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4681 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4741 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4801 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4861 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4921 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4981 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5041 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5101 ttttgtgatg ctcgtcaggg gggcggagct atggaaaaac gccagcaacg cgccttttta
     5161 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atccctgatt
     5221 ctgtggataa ccgtattacc gcctttgagt gctgataccg ctcgccgcag ccgaacgacc
     5281 gagcgcaagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     5341 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     5401 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     5461 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5521 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5581 cgaaacgcgc gaggcaggat cagccatatc acatttgtag aggttttact tgctttaaaa
     5641 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttaacttg
     5701 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5761 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5821 gtctggatca gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     5881 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct
     5941 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttcact
     6001 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcat
//
