LOCUS       Exported                6768 bp ds-DNA     circular SYN 26-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 26, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
COMMENT     Fragment extracted from plasmid.xdna [6379  nt] : (XhoI[31] / 
            HindIII[11])
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    51..66
                     /label=Ecdysone
     enhancer        96..105
                     /label=AaRel1A NFkB site
     protein_bind    156..174
                     /label=Ecdysone
     protein_bind    226..233
                     /label=Mtk GATA site
     enhancer        243..252
                     /label=AaRel1A NFkB site
     enhancer        complement(270..279)
                     /label=AaRel1A NFkB site
     protein_bind    complement(291..298)
                     /label=Mtk GATA site
     protein_bind    308..323
                     /label=Ecdysone
     protein_bind    complement(335..342)
                     /label=Mtk GATA site
     enhancer        complement(356..365)
                     /label=AaRel1A NFkB site
     protein_bind    408..423
                     /label=Ecdysone
     enhancer        453..462
                     /label=AaRel1A NFkB site
     protein_bind    513..531
                     /label=Ecdysone
     protein_bind    583..590
                     /label=Mtk GATA site
     enhancer        600..609
                     /label=AaRel1A NFkB site
     enhancer        complement(627..636)
                     /label=AaRel1A NFkB site
     protein_bind    complement(648..655)
                     /label=Mtk GATA site
     protein_bind    665..680
                     /label=Ecdysone
     protein_bind    complement(692..699)
                     /label=Mtk GATA site
     enhancer        complement(713..722)
                     /label=AaRel1A NFkB site
     protein_bind    765..780
                     /label=Ecdysone
     enhancer        810..819
                     /label=AaRel1A NFkB site
     protein_bind    870..888
                     /label=Ecdysone
     protein_bind    940..947
                     /label=Mtk GATA site
     enhancer        957..966
                     /label=AaRel1A NFkB site
     enhancer        complement(984..993)
                     /label=AaRel1A NFkB site
     protein_bind    complement(1005..1012)
                     /label=Mtk GATA site
     protein_bind    1022..1037
                     /label=Ecdysone
     protein_bind    complement(1049..1056)
                     /label=Mtk GATA site
     enhancer        complement(1070..1079)
                     /label=AaRel1A NFkB site
     enhancer        complement(1147..1156)
                     /label=AaRel1A NFkB site
     gene            1171..2868
                     /label=Firefly luciferase
     intron          2998..3013
                     /label=SV40 int
     protein_bind    3928..3936
                     /label=Mtk Rel site
     protein_bind    3947..3956
                     /label=Mtk Rel site
     promoter        4163..4191
                     /label=Amp prom
     CDS             4431..5090
                     /label=AmpR
     protein_bind    5180..5187
                     /label=Mtk GATA site
ORIGIN
        1 tcgagtctgg atccaagctt gcccgggcag atctctcgag gcgtggctcc gtgttgacgc
       61 atgttgacta tgcctttgaa tggctgccgt tcgagacgcg ggaaattcca acgtgcagaa
      121 aaaccaacag ggcgctaaaa aggagagtgt tttcgtggga ggtggagatg gtcactgggg
      181 gcaacataaa tattcagcga gaaacgtcat atttacattt agtctaggct gataatccgg
      241 ggacgcggga aattccaacg gggtggtgcc gttggaattt cccgcgtctc gatcggttat
      301 ctgcccccgg ctgacacttg cccgtcattc attcggctgc ttatcgcaga agctccgttg
      361 gaatttcccg cgtctcgaac tcgtttgtct ggtcgaggcg tggctccgtg ttgacgcatg
      421 ttgactatgc ctttgaatgg ctgccgttcg agacgcggga aattccaacg tgcagaaaaa
      481 ccaacagggc gctaaaaagg agagtgtttt cgtgggaggt ggagatggtc actgggggca
      541 acataaatat tcagcgagaa acgtcatatt tacatttagt ctaggctgat aatccgggga
      601 cgcgggaaat tccaacgggg tggtgccgtt ggaatttccc gcgtctcgat cggttatctg
      661 cccccggctg acacttgccc gtcattcatt cggctgctta tcgcagaagc tccgttggaa
      721 tttcccgcgt ctcgaactcg tttgtctggt cgaggcgtgg ctccgtgttg acgcatgttg
      781 actatgcctt tgaatggctg ccgttcgaga cgcgggaaat tccaacgtgc agaaaaacca
      841 acagggcgct aaaaaggaga gtgttttcgt gggaggtgga gatggtcact gggggcaaca
      901 taaatattca gcgagaaacg tcatatttac atttagtcta ggctgataat ccggggacgc
      961 gggaaattcc aacggggtgg tgccgttgga atttcccgcg tctcgatcgg ttatctgccc
     1021 ccggctgaca cttgcccgtc attcattcgg ctgcttatcg cagaagctcc gttggaattt
     1081 cccgcgtctc gaactcgttt gtctgggact gagctataaa agcctcacca tctcaacgct
     1141 caaagccgtt ggaatttccc gcgtccgagc taagatggga gctcgaattc cagcttggca
     1201 ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt
     1261 ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
     1321 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc
     1381 ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
     1441 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
     1501 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
     1561 gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca
     1621 aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc
     1681 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
     1741 cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact
     1801 gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac
     1861 tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
     1921 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
     1981 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt
     2041 acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt
     2101 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     2161 tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc
     2221 agggatacga caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     2281 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     2341 ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg
     2401 acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     2461 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     2521 agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt
     2581 ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga
     2641 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     2701 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     2761 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     2821 aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt aaaatgtaac
     2881 tgtattcagc gatgacgaaa ttcttagcta ttgtaatact ctagaggatc tttgtgaagg
     2941 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     3001 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     3061 tattttagat tccaacctat ggaacttatg aatgggagca gtggtggaat gcctttaatg
     3121 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     3181 ctcaacattc tactctcaaa agaagagaaa ggtagagacc caaggacttt ccttcagaat
     3241 tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt
     3301 acaaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     3361 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     3421 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     3481 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     3541 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     3601 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     3661 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3721 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccccaggaa
     3781 gctcctctgt gtcctcataa accctaacct cctctacttg agaggacatt ccaatcatag
     3841 gctgcccatc caccctctgt gtcctcctgt taattaggtc acttaacaaa aaggaaattg
     3901 ggtaggggtt tttcacagac cgctttctaa ggggtaattt taaaatatct gggaagtccc
     3961 ttccactgct gtgttccaga agtgttggta aacagcccac aaatgtcaac agcagaaaca
     4021 tacaagctgt cactttgcac aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4081 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4141 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4201 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4261 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4321 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4381 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4441 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4501 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4561 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4621 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4681 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4741 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4801 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4861 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4921 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4981 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5041 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5101 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5161 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5221 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5281 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5341 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5401 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5461 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5521 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5581 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5641 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5701 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5761 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5821 ttgtgatgct cgtcaggggg gcggagctat ggaaaaacgc cagcaacgcg cctttttacg
     5881 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat ccctgattct
     5941 gtggataacc gtattaccgc ctttgagtgc tgataccgct cgccgcagcc gaacgaccga
     6001 gcgcaagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     6061 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     6121 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     6181 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6241 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     6301 aaacgcgcga ggcaggatca gccatatcac atttgtagag gttttacttg ctttaaaaaa
     6361 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttaacttgtt
     6421 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6481 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6541 ctggatcagc catatcacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     6601 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt aacttgttta ttgcagctta
     6661 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttcactgc
     6721 attctagttg tggtttgtcc aaactcatca atgtatctta tcatagct
//
