LOCUS       Exported                7125 bp ds-DNA     circular SYN 26-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 26, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
COMMENT     Fragment extracted from plasmid.xdna [6379  nt] : (XhoI[31] / 
            HindIII[11])
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    51..66
                     /label=Ecdysone
     enhancer        96..105
                     /label=AaRel1A NFkB site
     protein_bind    156..174
                     /label=Ecdysone
     protein_bind    226..233
                     /label=Mtk GATA site
     enhancer        243..252
                     /label=AaRel1A NFkB site
     enhancer        complement(270..279)
                     /label=AaRel1A NFkB site
     protein_bind    complement(291..298)
                     /label=Mtk GATA site
     protein_bind    308..323
                     /label=Ecdysone
     protein_bind    complement(335..342)
                     /label=Mtk GATA site
     enhancer        complement(356..365)
                     /label=AaRel1A NFkB site
     protein_bind    408..423
                     /label=Ecdysone
     enhancer        453..462
                     /label=AaRel1A NFkB site
     protein_bind    513..531
                     /label=Ecdysone
     protein_bind    583..590
                     /label=Mtk GATA site
     enhancer        600..609
                     /label=AaRel1A NFkB site
     enhancer        complement(627..636)
                     /label=AaRel1A NFkB site
     protein_bind    complement(648..655)
                     /label=Mtk GATA site
     protein_bind    665..680
                     /label=Ecdysone
     protein_bind    complement(692..699)
                     /label=Mtk GATA site
     enhancer        complement(713..722)
                     /label=AaRel1A NFkB site
     protein_bind    765..780
                     /label=Ecdysone
     enhancer        810..819
                     /label=AaRel1A NFkB site
     protein_bind    870..888
                     /label=Ecdysone
     protein_bind    940..947
                     /label=Mtk GATA site
     enhancer        957..966
                     /label=AaRel1A NFkB site
     enhancer        complement(984..993)
                     /label=AaRel1A NFkB site
     protein_bind    complement(1005..1012)
                     /label=Mtk GATA site
     protein_bind    1022..1037
                     /label=Ecdysone
     protein_bind    complement(1049..1056)
                     /label=Mtk GATA site
     enhancer        complement(1070..1079)
                     /label=AaRel1A NFkB site
     protein_bind    1122..1137
                     /label=Ecdysone
     enhancer        1167..1176
                     /label=AaRel1A NFkB site
     protein_bind    1227..1245
                     /label=Ecdysone
     protein_bind    1297..1304
                     /label=Mtk GATA site
     enhancer        1314..1323
                     /label=AaRel1A NFkB site
     enhancer        complement(1341..1350)
                     /label=AaRel1A NFkB site
     protein_bind    complement(1362..1369)
                     /label=Mtk GATA site
     protein_bind    1379..1394
                     /label=Ecdysone
     protein_bind    complement(1406..1413)
                     /label=Mtk GATA site
     enhancer        complement(1427..1436)
                     /label=AaRel1A NFkB site
     enhancer        complement(1504..1513)
                     /label=AaRel1A NFkB site
     gene            1528..3225
                     /label=Firefly luciferase
     intron          3355..3370
                     /label=SV40 int
     protein_bind    4285..4293
                     /label=Mtk Rel site
     protein_bind    4304..4313
                     /label=Mtk Rel site
     promoter        4520..4548
                     /label=Amp prom
     CDS             4788..5447
                     /label=AmpR
     protein_bind    5537..5544
                     /label=Mtk GATA site
ORIGIN
        1 tcgagtctgg atccaagctt gcccgggcag atctctcgag gcgtggctcc gtgttgacgc
       61 atgttgacta tgcctttgaa tggctgccgt tcgagacgcg ggaaattcca acgtgcagaa
      121 aaaccaacag ggcgctaaaa aggagagtgt tttcgtggga ggtggagatg gtcactgggg
      181 gcaacataaa tattcagcga gaaacgtcat atttacattt agtctaggct gataatccgg
      241 ggacgcggga aattccaacg gggtggtgcc gttggaattt cccgcgtctc gatcggttat
      301 ctgcccccgg ctgacacttg cccgtcattc attcggctgc ttatcgcaga agctccgttg
      361 gaatttcccg cgtctcgaac tcgtttgtct ggtcgaggcg tggctccgtg ttgacgcatg
      421 ttgactatgc ctttgaatgg ctgccgttcg agacgcggga aattccaacg tgcagaaaaa
      481 ccaacagggc gctaaaaagg agagtgtttt cgtgggaggt ggagatggtc actgggggca
      541 acataaatat tcagcgagaa acgtcatatt tacatttagt ctaggctgat aatccgggga
      601 cgcgggaaat tccaacgggg tggtgccgtt ggaatttccc gcgtctcgat cggttatctg
      661 cccccggctg acacttgccc gtcattcatt cggctgctta tcgcagaagc tccgttggaa
      721 tttcccgcgt ctcgaactcg tttgtctggt cgaggcgtgg ctccgtgttg acgcatgttg
      781 actatgcctt tgaatggctg ccgttcgaga cgcgggaaat tccaacgtgc agaaaaacca
      841 acagggcgct aaaaaggaga gtgttttcgt gggaggtgga gatggtcact gggggcaaca
      901 taaatattca gcgagaaacg tcatatttac atttagtcta ggctgataat ccggggacgc
      961 gggaaattcc aacggggtgg tgccgttgga atttcccgcg tctcgatcgg ttatctgccc
     1021 ccggctgaca cttgcccgtc attcattcgg ctgcttatcg cagaagctcc gttggaattt
     1081 cccgcgtctc gaactcgttt gtctggtcga ggcgtggctc cgtgttgacg catgttgact
     1141 atgcctttga atggctgccg ttcgagacgc gggaaattcc aacgtgcaga aaaaccaaca
     1201 gggcgctaaa aaggagagtg ttttcgtggg aggtggagat ggtcactggg ggcaacataa
     1261 atattcagcg agaaacgtca tatttacatt tagtctaggc tgataatccg gggacgcggg
     1321 aaattccaac ggggtggtgc cgttggaatt tcccgcgtct cgatcggtta tctgcccccg
     1381 gctgacactt gcccgtcatt cattcggctg cttatcgcag aagctccgtt ggaatttccc
     1441 gcgtctcgaa ctcgtttgtc tgggactgag ctataaaagc ctcaccatct caacgctcaa
     1501 agccgttgga atttcccgcg tccgagctaa gatgggagct cgaattccag cttggcattc
     1561 cggtactgtt ggtaaaatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
     1621 tcctctagag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
     1681 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga
     1741 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
     1801 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
     1861 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
     1921 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa
     1981 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa
     2041 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
     2101 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat
     2161 aatgaattcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc
     2221 ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
     2281 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
     2341 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg
     2401 atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt
     2461 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
     2521 gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg
     2581 gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
     2641 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
     2701 tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc
     2761 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
     2821 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt
     2881 tgaccgcttg aagtctttaa ttaaatacaa aggatatcag gtggcccccg ctgaattgga
     2941 atcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga
     3001 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
     3061 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
     3121 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
     3181 cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgtaaa atgtaactgt
     3241 attcagcgat gacgaaattc ttagctattg taatactcta gaggatcttt gtgaaggaac
     3301 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     3361 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     3421 tttagattcc aacctatgga acttatgaat gggagcagtg gtggaatgcc tttaatgagg
     3481 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     3541 aacattctac tctcaaaaga agagaaaggt agagacccaa ggactttcct tcagaattgc
     3601 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     3661 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     3721 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     3781 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     3841 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     3901 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3961 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     4021 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4081 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ccaggaagct
     4141 cctctgtgtc ctcataaacc ctaacctcct ctacttgaga ggacattcca atcataggct
     4201 gcccatccac cctctgtgtc ctcctgttaa ttaggtcact taacaaaaag gaaattgggt
     4261 aggggttttt cacagaccgc tttctaaggg gtaattttaa aatatctggg aagtcccttc
     4321 cactgctgtg ttccagaagt gttggtaaac agcccacaaa tgtcaacagc agaaacatac
     4381 aagctgtcac tttgcacaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4441 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4501 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4561 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4621 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4681 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4741 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4801 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4861 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4921 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4981 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5041 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5101 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5161 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5221 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5281 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5341 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5401 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5461 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5521 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5581 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5641 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5701 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5761 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5821 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5881 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5941 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6001 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6061 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6121 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6181 tgatgctcgt caggggggcg gagctatgga aaaacgccag caacgcgcct ttttacggtt
     6241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc tgattctgtg
     6301 gataaccgta ttaccgcctt tgagtgctga taccgctcgc cgcagccgaa cgaccgagcg
     6361 caagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     6421 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     6481 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     6541 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6601 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6661 cgcgcgaggc aggatcagcc atatcacatt tgtagaggtt ttacttgctt taaaaaacct
     6721 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgtta acttgtttat
     6781 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6841 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6901 gatcagccat atcacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     6961 cctgaacctg aaacataaaa tgaatgcaat tgttgttaac ttgtttattg cagcttataa
     7021 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tcactgcatt
     7081 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tagct
//
