LOCUS       Exported                7482 bp ds-DNA     circular SYN 26-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 26, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
COMMENT     Fragment extracted from plasmid.xdna [6379  nt] : (XhoI[31] / 
            HindIII[11])
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    51..66
                     /label=Ecdysone
     enhancer        96..105
                     /label=AaRel1A NFkB site
     protein_bind    156..174
                     /label=Ecdysone
     protein_bind    226..233
                     /label=Mtk GATA site
     enhancer        243..252
                     /label=AaRel1A NFkB site
     enhancer        complement(270..279)
                     /label=AaRel1A NFkB site
     protein_bind    complement(291..298)
                     /label=Mtk GATA site
     protein_bind    308..323
                     /label=Ecdysone
     protein_bind    complement(335..342)
                     /label=Mtk GATA site
     enhancer        complement(356..365)
                     /label=AaRel1A NFkB site
     protein_bind    408..423
                     /label=Ecdysone
     enhancer        453..462
                     /label=AaRel1A NFkB site
     protein_bind    513..531
                     /label=Ecdysone
     protein_bind    583..590
                     /label=Mtk GATA site
     enhancer        600..609
                     /label=AaRel1A NFkB site
     enhancer        complement(627..636)
                     /label=AaRel1A NFkB site
     protein_bind    complement(648..655)
                     /label=Mtk GATA site
     protein_bind    665..680
                     /label=Ecdysone
     protein_bind    complement(692..699)
                     /label=Mtk GATA site
     enhancer        complement(713..722)
                     /label=AaRel1A NFkB site
     protein_bind    765..780
                     /label=Ecdysone
     enhancer        810..819
                     /label=AaRel1A NFkB site
     protein_bind    870..888
                     /label=Ecdysone
     protein_bind    940..947
                     /label=Mtk GATA site
     enhancer        957..966
                     /label=AaRel1A NFkB site
     enhancer        complement(984..993)
                     /label=AaRel1A NFkB site
     protein_bind    complement(1005..1012)
                     /label=Mtk GATA site
     protein_bind    1022..1037
                     /label=Ecdysone
     protein_bind    complement(1049..1056)
                     /label=Mtk GATA site
     enhancer        complement(1070..1079)
                     /label=AaRel1A NFkB site
     protein_bind    1122..1137
                     /label=Ecdysone
     enhancer        1167..1176
                     /label=AaRel1A NFkB site
     protein_bind    1227..1245
                     /label=Ecdysone
     protein_bind    1297..1304
                     /label=Mtk GATA site
     enhancer        1314..1323
                     /label=AaRel1A NFkB site
     enhancer        complement(1341..1350)
                     /label=AaRel1A NFkB site
     protein_bind    complement(1362..1369)
                     /label=Mtk GATA site
     protein_bind    1379..1394
                     /label=Ecdysone
     protein_bind    complement(1406..1413)
                     /label=Mtk GATA site
     enhancer        complement(1427..1436)
                     /label=AaRel1A NFkB site
     protein_bind    1479..1494
                     /label=Ecdysone
     enhancer        1524..1533
                     /label=AaRel1A NFkB site
     protein_bind    1584..1602
                     /label=Ecdysone
     protein_bind    1654..1661
                     /label=Mtk GATA site
     enhancer        1671..1680
                     /label=AaRel1A NFkB site
     enhancer        complement(1698..1707)
                     /label=AaRel1A NFkB site
     protein_bind    complement(1719..1726)
                     /label=Mtk GATA site
     protein_bind    1736..1751
                     /label=Ecdysone
     protein_bind    complement(1763..1770)
                     /label=Mtk GATA site
     enhancer        complement(1784..1793)
                     /label=AaRel1A NFkB site
     enhancer        complement(1861..1870)
                     /label=AaRel1A NFkB site
     gene            1885..3582
                     /label=Firefly luciferase
     intron          3712..3727
                     /label=SV40 int
     protein_bind    4642..4650
                     /label=Mtk Rel site
     protein_bind    4661..4670
                     /label=Mtk Rel site
     promoter        4877..4905
                     /label=Amp prom
     CDS             5145..5804
                     /label=AmpR
     protein_bind    5894..5901
                     /label=Mtk GATA site
ORIGIN
        1 tcgagtctgg atccaagctt gcccgggcag atctctcgag gcgtggctcc gtgttgacgc
       61 atgttgacta tgcctttgaa tggctgccgt tcgagacgcg ggaaattcca acgtgcagaa
      121 aaaccaacag ggcgctaaaa aggagagtgt tttcgtggga ggtggagatg gtcactgggg
      181 gcaacataaa tattcagcga gaaacgtcat atttacattt agtctaggct gataatccgg
      241 ggacgcggga aattccaacg gggtggtgcc gttggaattt cccgcgtctc gatcggttat
      301 ctgcccccgg ctgacacttg cccgtcattc attcggctgc ttatcgcaga agctccgttg
      361 gaatttcccg cgtctcgaac tcgtttgtct ggtcgaggcg tggctccgtg ttgacgcatg
      421 ttgactatgc ctttgaatgg ctgccgttcg agacgcggga aattccaacg tgcagaaaaa
      481 ccaacagggc gctaaaaagg agagtgtttt cgtgggaggt ggagatggtc actgggggca
      541 acataaatat tcagcgagaa acgtcatatt tacatttagt ctaggctgat aatccgggga
      601 cgcgggaaat tccaacgggg tggtgccgtt ggaatttccc gcgtctcgat cggttatctg
      661 cccccggctg acacttgccc gtcattcatt cggctgctta tcgcagaagc tccgttggaa
      721 tttcccgcgt ctcgaactcg tttgtctggt cgaggcgtgg ctccgtgttg acgcatgttg
      781 actatgcctt tgaatggctg ccgttcgaga cgcgggaaat tccaacgtgc agaaaaacca
      841 acagggcgct aaaaaggaga gtgttttcgt gggaggtgga gatggtcact gggggcaaca
      901 taaatattca gcgagaaacg tcatatttac atttagtcta ggctgataat ccggggacgc
      961 gggaaattcc aacggggtgg tgccgttgga atttcccgcg tctcgatcgg ttatctgccc
     1021 ccggctgaca cttgcccgtc attcattcgg ctgcttatcg cagaagctcc gttggaattt
     1081 cccgcgtctc gaactcgttt gtctggtcga ggcgtggctc cgtgttgacg catgttgact
     1141 atgcctttga atggctgccg ttcgagacgc gggaaattcc aacgtgcaga aaaaccaaca
     1201 gggcgctaaa aaggagagtg ttttcgtggg aggtggagat ggtcactggg ggcaacataa
     1261 atattcagcg agaaacgtca tatttacatt tagtctaggc tgataatccg gggacgcggg
     1321 aaattccaac ggggtggtgc cgttggaatt tcccgcgtct cgatcggtta tctgcccccg
     1381 gctgacactt gcccgtcatt cattcggctg cttatcgcag aagctccgtt ggaatttccc
     1441 gcgtctcgaa ctcgtttgtc tggtcgaggc gtggctccgt gttgacgcat gttgactatg
     1501 cctttgaatg gctgccgttc gagacgcggg aaattccaac gtgcagaaaa accaacaggg
     1561 cgctaaaaag gagagtgttt tcgtgggagg tggagatggt cactgggggc aacataaata
     1621 ttcagcgaga aacgtcatat ttacatttag tctaggctga taatccgggg acgcgggaaa
     1681 ttccaacggg gtggtgccgt tggaatttcc cgcgtctcga tcggttatct gcccccggct
     1741 gacacttgcc cgtcattcat tcggctgctt atcgcagaag ctccgttgga atttcccgcg
     1801 tctcgaactc gtttgtctgg gactgagcta taaaagcctc accatctcaa cgctcaaagc
     1861 cgttggaatt tcccgcgtcc gagctaagat gggagctcga attccagctt ggcattccgg
     1921 tactgttggt aaaatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
     1981 tctagaggat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
     2041 tcctggaaca attgctttta cagatgcaca tatcgaggtg aacatcacgt acgcggaata
     2101 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
     2161 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
     2221 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
     2281 cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat
     2341 tttgaacgtg caaaaaaaat taccaataat ccagaaaatt attatcatgg attctaaaac
     2401 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
     2461 taatgaatac gattttgtac cagagtcctt tgatcgtgac aaaacaattg cactgataat
     2521 gaattcctct ggatctactg ggttacctaa gggtgtggcc cttccgcata gaactgcctg
     2581 cgtcagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
     2641 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
     2701 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttttacgatc
     2761 ccttcaggat tacaaaattc aaagtgcgtt gctagtacca accctatttt cattcttcgc
     2821 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggggg
     2881 cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc ttccagggat
     2941 acgacaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     3001 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     3061 ggataccggg aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca gaggacctat
     3121 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     3181 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatagttga
     3241 ccgcttgaag tctttaatta aatacaaagg atatcaggtg gcccccgctg aattggaatc
     3301 gatattgtta caacacccca acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga
     3361 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     3421 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     3481 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     3541 agagatcctc ataaaggcca agaagggcgg aaagtccaaa ttgtaaaatg taactgtatt
     3601 cagcgatgac gaaattctta gctattgtaa tactctagag gatctttgtg aaggaacctt
     3661 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
     3721 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
     3781 agattccaac ctatggaact tatgaatggg agcagtggtg gaatgccttt aatgaggaaa
     3841 acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac
     3901 attctactct caaaagaaga gaaaggtaga gacccaagga ctttccttca gaattgctaa
     3961 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacaacc
     4021 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     4081 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     4141 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     4201 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     4261 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     4321 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     4381 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4441 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcccca ggaagctcct
     4501 ctgtgtcctc ataaacccta acctcctcta cttgagagga cattccaatc ataggctgcc
     4561 catccaccct ctgtgtcctc ctgttaatta ggtcacttaa caaaaaggaa attgggtagg
     4621 ggtttttcac agaccgcttt ctaaggggta attttaaaat atctgggaag tcccttccac
     4681 tgctgtgttc cagaagtgtt ggtaaacagc ccacaaatgt caacagcaga aacatacaag
     4741 ctgtcacttt gcacaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4801 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4861 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4921 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4981 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5041 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5101 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5161 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5221 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5281 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5341 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5401 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5461 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5521 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5581 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5641 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5701 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5761 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5821 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5881 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5941 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6001 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6061 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6361 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6541 tgctcgtcag gggggcggag ctatggaaaa acgccagcaa cgcgcctttt tacggttcct
     6601 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatccctga ttctgtggat
     6661 aaccgtatta ccgcctttga gtgctgatac cgctcgccgc agccgaacga ccgagcgcaa
     6721 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     6781 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6841 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     6901 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6961 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     7021 gcgaggcagg atcagccata tcacatttgt agaggtttta cttgctttaa aaaacctccc
     7081 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttaact tgtttattgc
     7141 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7201 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     7261 cagccatatc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7321 gaacctgaaa cataaaatga atgcaattgt tgttaacttg tttattgcag cttataatgg
     7381 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcatttttca ctgcattcta
     7441 gttgtggttt gtccaaactc atcaatgtat cttatcatag ct
//
