LOCUS       Exported                6609 bp ds-DNA     circular SYN 26-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 26, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..482
                     /label=AcNPV hr5 enhancer
     promoter        487..1082
                     /label=AcNPV IE1 promoter
     polyA_site      961..967
                     /label=Rabbit B-globin polyA
     CDS             1087..1110
                     /label=Flag tag
     gene            1111..4092
                     /label=AaRel2-long
     terminator      4126..4433
                     /label=AcNPV IE1 terminator
     promoter        4498..4526
                     /label=Amp prom
     CDS             4766..5425
                     /label=AmpR
     rep_origin      5577..6205
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        6511..6540
                     /label=lac
     misc_binding    6545..6567
                     /label=LacO
     primer_bind     6573..6593
                     /label=M13-rev
ORIGIN
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
      781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
     1081 accatggatt acaaggatga cgatgacaag tcaacactac tcaatttgga tagctaccgg
     1141 catgagttat tcgagcacca tcagcatcag cagcagcatc accagctcca gcagctttcg
     1201 tcatcgccga cgtattcggt gctctcgatg gaatctgtat cgcccgtgtc gtcatcatcg
     1261 gcaagccccg cagccgacac tagcaaatac tacgcgagca actctccgtc cagtgtcagt
     1321 aatatgtccc ccaaatcaac agctagtgat acgagctcct tcaacatgca gaatctgaac
     1381 atctccaacg cctacccgta ctttgccgag actcatcatc agacgatgac aaccggcgaa
     1441 gagggtcaac tgcagacctt tacctataat gatccaaccc agcctcgagt ggagctttcg
     1501 gtacctcagc taacgatcgt tgagcaaccg gtggacaaat tccgcttccg ataccagtcg
     1561 gagatgcacg gaacacatgg gtccctgatg ggggtccata cggagaaaag caagaaaacc
     1621 ttccccaccg tgcagctgca tgggtttcag ggcgaggcca agattcgatg ttcgctgttc
     1681 caggtggacc cgaaccggag ggctgcccat tcgcacaacc tggtgatcaa gagtggcgaa
     1741 atagatttga acgatccgca tgacatcgat gtgaacgaag agtgcaacta tgtggccatg
     1801 ttccagggga tgggcatcat acacacggcc aaaaagaata tcgccgagga gttgagcaag
     1861 aagatgaaga agcaacggtc ggtggagatg aaccgggagc tgaccctcag ggaagagtac
     1921 cagttgcaga aggaagcggc cgaaatggcc aagacgatga atctgaatca ggtgtgcctg
     1981 tgcttccagg cgttccaggt ggatgcctcg accggtcggt gggcgcagct ctgcgaaccg
     2041 gtttattcca atccgattaa taatatgaag agtgccctca cgggggaatt gaagatctgc
     2101 cggctcagtg ctaccgtggg aaacgttgac ggcggcgaag aggtgttcat gttcgttgaa
     2161 aaagtttgca aaaataacat caagatccgg ttctatgaac tggacgaata cgatcaggag
     2221 atctggcaag actggggttc cttctcggaa gctgacgtcc atcatcaata tgcgatcgca
     2281 ttcaaaactc ccgcttatca caacaaagac atcaccgaac cggtggaagt gctcatgcag
     2341 ctctaccggc cacgagacaa atgccagagc gaacccgtcc cgttcaaatt caagccccgg
     2401 ttcaacatat ctcggaagcg cccgagagtc agttccggct tactttcgaa tgagattcca
     2461 acagtagttc ccaatgaacc gggatcgagc cgacttccgt cgttccatca tccgttttcc
     2521 atgatgcccc cgatgcaagc gcccatcgcc gaggccagtg gaagcagcag tagccacacc
     2581 atcagcaaag agtacaacaa atccggtatc atccaggaga ttttggacag ccacattccg
     2641 accacaattg ccggcgatat atcgttcagc tcgaacgact tcaaagaatt cgtcaattgc
     2701 aactcggaag aattacgcaa gctaataacg gagatcgggg aagcgcagga ggagtccaag
     2761 ctggagacgg acgcggtatc ggttggtcac gtggacgagg cagcggcgca gtttgagcgc
     2821 acgctagagc agtacctgga gcacaacagc caagccaagg acgtggagat tctgaagaag
     2881 atactggcca tcatacggtt actgttccgg aagaactacg accagtgccg ggaacttatc
     2941 tcggccctct ggatgtcggc caacaaactt aaagccaatt gtctacacat ggcgattgaa
     3001 cgacgcaaca tcaccatcgc ttgtaagctg gtggaactcc tgcaggacca ccacctcctg
     3061 gacatgctcg atcttttcaa cgaacgcaac gaaaccgccc tgcatttggc cgtttcggcc
     3121 aatctggtag aggtcgtcga cgtcctgctc ctaaccggtt cccggattag ctactgtgac
     3181 agccggggga actccgctct gcatcgggcc gtgtatgaga acgccttgga tagtttgaac
     3241 gtgctgttgg gtcattgcaa acggaacggg ttccggttgg attcgacaaa tgatgatgga
     3301 ttcacggcgc tgcatttggc ggtgatgtgt aaaaatttga aggccacgaa ggttctcctg
     3361 gatcggggcg cttcgtacgt gctgcgtgat cttaagcatg gaaacaacat tcttcatatt
     3421 gccgtggaga gcgattcttt ggacatggtc aattttatcc ttgagggtgt agacaagacg
     3481 ttagctgatg aaccgaacaa tgccggttac acaccacttc agctggctaa cgcaaggaat
     3541 ttggcaaacg cgaacaacaa actaattgtc cgcgaattgc ttcgatataa tccaagcgga
     3601 gtactagaaa aagaacatct caccgaagaa gaagacgacg atcaagaaca ggaagagaac
     3661 cttcccccta gcagcgattc gcagtctgaa accgtgctcg agtcgatgaa tgtgagctgt
     3721 aatagagtgg aggttatacg gttactagaa gactattctc caccgaacga tgaaccaaag
     3781 ttaacaccgc tggagaacgt tccttacaat agcaccctgg aggatggatc cgtctatctg
     3841 tttgatgagg tgtgcttgtc tcacctgtgc ggtcttctga accgaaagaa cctttggcgc
     3901 gaggttgggt cgcttctgga cttcaactca ttcttcatga tctgggaaac gtcggtgaac
     3961 cccgcaggga tgttgctgaa ctactttgag atgcaaaaag tgaaactcga gcacttgatc
     4021 gatgtgctgc aagctttgga ccaaaaagaa gccattcact acatcgacga gatgatttgt
     4081 cggcagatga agtaataact cgagcactaa gtgattaacc tcaggttata catatatttt
     4141 gaatttaatt aattatacat atattttata ttatttttgt cttttattat cgaggggccg
     4201 ttgttggtgt ggggttttgc atagaaataa caatgggagt tggcgacgtt gctgcgccaa
     4261 caccacctcc cttccctcct ttcatcatgt atctgtagat aaaataaaat attaaaccta
     4321 aaaacaagac cgcgcctatc aacaaaatga taggcattaa cttgccgctg acgctgtcac
     4381 taacgttgga cgatttgccg actaaacctt catcgcccag taaccaatct agacgtcagg
     4441 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4501 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4561 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4621 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4681 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4741 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4801 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4861 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4921 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4981 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5041 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5101 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5161 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5221 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5281 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5341 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5401 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5461 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5521 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5581 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5641 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5701 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     5761 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5821 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5881 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5941 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6001 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6061 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6121 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6181 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6241 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6301 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6361 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6421 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6481 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6541 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga
     6601 attcccggg
//
