LOCUS       Exported                5305 bp ds-DNA     circular SYN 26-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 26, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..482
                     /label=AcNPV hr5 enhancer
     promoter        487..1082
                     /label=AcNPV IE1 promoter
     polyA_site      961..967
                     /label=Rabbit B-globin polyA
     CDS             1087..1110
                     /label=Flag tag
     gene            1111..2775
                     /label=AaRel2-long-dIkB
     terminator      2822..3129
                     /label=AcNPV IE1 terminator
     promoter        3194..3222
                     /label=Amp prom
     CDS             3462..4121
                     /label=AmpR
     rep_origin      4273..4901
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        5207..5236
                     /label=lac
     misc_binding    5241..5263
                     /label=LacO
     primer_bind     5269..5289
                     /label=M13-rev
ORIGIN
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
      781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
     1081 accatggatt acaaggatga cgatgacaag tcaacactac tcaatttgga tagctaccgg
     1141 catgagttat tcgagcacca tcagcatcag cagcagcatc accagctcca gcagctttcg
     1201 tcatcgccga cgtattcggt gctctcgatg gaatctgtat cgcccgtgtc gtcatcatcg
     1261 gcaagccccg cagccgacac tagcaaatac tacgcgagca actctccgtc cagtgtcagt
     1321 aatatgtccc ccaaatcaac agctagtgat acgagctcct tcaacatgca gaatctgaac
     1381 atctccaacg cctacccgta ctttgccgag actcatcatc agacgatgac aaccggcgaa
     1441 gagggtcaac tgcagacctt tacctataat gatccaaccc agcctcgagt ggagctttcg
     1501 gtacctcagc taacgatcgt tgagcaaccg gtggacaaat tccgcttccg ataccagtcg
     1561 gagatgcacg gaacacatgg gtccctgatg ggggtccata cggagaaaag caagaaaacc
     1621 ttccccaccg tgcagctgca tgggtttcag ggcgaggcca agattcgatg ttcgctgttc
     1681 caggtggacc cgaaccggag ggctgcccat tcgcacaacc tggtgatcaa gagtggcgaa
     1741 atagatttga acgatccgca tgacatcgat gtgaacgaag agtgcaacta tgtggccatg
     1801 ttccagggga tgggcatcat acacacggcc aaaaagaata tcgccgagga gttgagcaag
     1861 aagatgaaga agcaacggtc ggtggagatg aaccgggagc tgaccctcag ggaagagtac
     1921 cagttgcaga aggaagcggc cgaaatggcc aagacgatga atctgaatca ggtgtgcctg
     1981 tgcttccagg cgttccaggt ggatgcctcg accggtcggt gggcgcagct ctgcgaaccg
     2041 gtttattcca atccgattaa taatatgaag agtgccctca cgggggaatt gaagatctgc
     2101 cggctcagtg ctaccgtggg aaacgttgac ggcggcgaag aggtgttcat gttcgttgaa
     2161 aaagtttgca aaaataacat caagatccgg ttctatgaac tggacgaata cgatcaggag
     2221 atctggcaag actggggttc cttctcggaa gctgacgtcc atcatcaata tgcgatcgca
     2281 ttcaaaactc ccgcttatca caacaaagac atcaccgaac cggtggaagt gctcatgcag
     2341 ctctaccggc cacgagacaa atgccagagc gaacccgtcc cgttcaaatt caagccccgg
     2401 ttcaacatat ctcggaagcg cccgagagtc agttccggct tactttcgaa tgagattcca
     2461 acagtagttc ccaatgaacc gggatcgagc cgacttccgt cgttccatca tccgttttcc
     2521 atgatgcccc cgatgcaagc gcccatcgcc gaggccagtg gaagcagcag tagccacacc
     2581 atcagcaaag agtacaacaa atccggtatc atccaggaga ttttggacag ccacattccg
     2641 accacaattg ccggcgatat atcgttcagc tcgaacgact tcaaagaatt cgtcaattgc
     2701 aactcggaag aattacgcaa gctaataacg gagatcgggg aagcgcagga ggagtccaag
     2761 ctggagacgg actaactcga gcactaagtg ataactcgag cactaagtga ttaacctcag
     2821 gttatacata tattttgaat ttaattaatt atacatatat tttatattat ttttgtcttt
     2881 tattatcgag gggccgttgt tggtgtgggg ttttgcatag aaataacaat gggagttggc
     2941 gacgttgctg cgccaacacc acctcccttc cctcctttca tcatgtatct gtagataaaa
     3001 taaaatatta aacctaaaaa caagaccgcg cctatcaaca aaatgatagg cattaacttg
     3061 ccgctgacgc tgtcactaac gttggacgat ttgccgacta aaccttcatc gcccagtaac
     3121 caatctagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3181 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3241 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     3301 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3361 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     3421 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     3481 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     3541 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     3601 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     3661 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     3721 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     3781 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     3841 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3901 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3961 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4021 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4081 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4141 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4201 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4261 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4321 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4381 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4441 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4501 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4561 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4621 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4681 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4741 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4801 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4861 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4921 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4981 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5041 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5101 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5161 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     5221 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     5281 atgaccatga ttacgaattc ccggg
//
