LOCUS       Exported                3624 bp ds-DNA     circular SYN 26-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 26, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..482
                     /label=AcNPV hr5 enhancer
     promoter        487..1082
                     /label=AcNPV IE1 promoter
     polyA_site      961..967
                     /label=Rabbit B-globin polyA
     CDS             1087..1110
                     /label=Flag tag
     terminator      1141..1448
                     /label=AcNPV IE1 terminator
     promoter        1513..1541
                     /label=Amp prom
     CDS             1781..2440
                     /label=AmpR
     rep_origin      2592..3220
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        3526..3555
                     /label=lac
     misc_binding    3560..3582
                     /label=LacO
     primer_bind     3588..3608
                     /label=M13-rev
ORIGIN
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
      781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
     1081 accatggatt acaaggatga cgatgacaag taactcgagc actaagtgat taacctcagg
     1141 ttatacatat attttgaatt taattaatta tacatatatt ttatattatt tttgtctttt
     1201 attatcgagg ggccgttgtt ggtgtggggt tttgcataga aataacaatg ggagttggcg
     1261 acgttgctgc gccaacacca cctcccttcc ctcctttcat catgtatctg tagataaaat
     1321 aaaatattaa acctaaaaac aagaccgcgc ctatcaacaa aatgataggc attaacttgc
     1381 cgctgacgct gtcactaacg ttggacgatt tgccgactaa accttcatcg cccagtaacc
     1441 aatctagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1501 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1561 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1621 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1681 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1741 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1801 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     1861 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1921 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1981 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2041 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2101 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2161 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2221 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2281 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2341 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2401 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2461 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2521 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2581 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2641 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2701 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2761 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2821 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2881 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2941 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3001 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3061 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3121 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3181 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3241 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3301 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3361 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     3421 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     3481 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     3541 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     3601 tgaccatgat tacgaattcc cggg
//
