LOCUS       Exported                6124 bp ds-DNA     circular SYN 26-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Path-IT
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 26, 2020 from SnapGene 5.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    769..789
                     /label=CMV promoter
                     /note="ApEinfo_fwdcolor=#ff2249ApEinfo_graphicformat=arrow_
                     data {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=green"
                     /note="color: #ff2249"
     primer_bind     863..882
                     /label=T7
                     /note="ApEinfo_fwdcolor=#2054ffApEinfo_graphicformat=arrow_
                     data {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=green"
                     /note="color: #2054ff; direction: RIGHT"
     misc_feature    914
                     /label=Apa1
                     /note="ApEinfo_fwdcolor=#fffa63ApEinfo_graphicformat=arrow_
                     data {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=green"
                     /note="color: #fffa63"
     misc_feature    915..923
                     /label=KoZAK
                     /note="ApEinfo_fwdcolor=#ffa9a4ApEinfo_graphicformat=arrow_
                     data {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=green"
                     /note="color: #ffa9a4"
     CDS             924..1595
                     /codon_start=1
                     /label=coding
                     /note="ApEinfo_fwdcolor=cyanApEinfo_graphicformat=arrow_dat
                     a {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=green"
                     /note="color: #00ffff"
                     /translation="MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVK
                     RSVKDAAKKGQKEVCVVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSL
                     QKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEAAEM
                     EIDRILFEITAGALGKAPSKVTDALPEPEPAGAMAASEEGEEEEDEEDLEAMQSRLATL
                     RS"
     misc_feature    1596..1622
                     /label=HA TAG
                     /note="ApEinfo_fwdcolor=#ff98ccApEinfo_graphicformat=arrow_
                     data {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=green"
                     /note="color: #ff98cc"
     misc_feature    1623..1625
                     /label=STOP
                     /note="ApEinfo_fwdcolor=#ff1829ApEinfo_graphicformat=arrow_
                     data {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=green"
                     /note="color: #ff1829"
     misc_feature    1626
                     /label=HindIII
                     /note="ApEinfo_fwdcolor=#fdff67ApEinfo_graphicformat=arrow_
                     data {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=green"
                     /note="color: #fdff67"
     rep_origin      2008..2314
                     /label=F1 ori
                     /note="ApEinfo_fwdcolor=gray50ApEinfo_graphicformat=arrow_d
                     ata {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=gray50"
                     /note="color: #808080"
     CDS             2832..3623
                     /label=Kan/neoR
                     /note="ApEinfo_fwdcolor=#ff500fApEinfo_graphicformat=arrow_
                     data {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=yellow"
                     /note="color: #ff500f; direction: RIGHT"
     primer_bind     complement(3963..3983)
                     /label=M13-rev
                     /note="ApEinfo_fwdcolor=cyanApEinfo_graphicformat=arrow_dat
                     a {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     misc_binding    complement(3989..4011)
                     /label=LacO
                     /note="ApEinfo_fwdcolor=#6495edApEinfo_graphicformat=arrow_
                     data {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=#6495ed"
                     /note="color: #6495ed; direction: LEFT"
     rep_origin      complement(4351..5033)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ApEinfo_fwdcolor=gray50ApEinfo_graphicformat=arrow_d
                     ata {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=gray50"
                     /note="color: #808080"
     CDS             complement(5131..5790)
                     /label=AmpR
                     /note="ApEinfo_fwdcolor=#ff6b14ApEinfo_graphicformat=arrow_
                     data {{0 1 2 0 0 -1} {} 0} width 5 offset 
                     0ApEinfo_revcolor=#ff8a12"
                     /note="color: #ff8a12; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccccaattt gccatgggac tctttggaaa aacccaggag aagcctccca
      961 aagagctggt taatgaatgg tcactgaaga tcagaaagga aatgagagtt gttgacaggc
     1021 aaataagaga tatccaaaga gaagaagaga aagtaaaacg gtctgtgaaa gatgcagcca
     1081 agaagggcca aaaggaagtc tgtgtggttc tggccaagga gatgatcagg tcaaggaaag
     1141 ccgtgagcaa gctctatgcc tccaaagcac acatgaactc tgtgctcatg ggcatgaaga
     1201 accaactcgc ggtcctgcga gtagctggtt ctctgcagaa gagcacagaa gtgatgaagg
     1261 caatgcagag tcttgtgaag atcccagaaa tccaggccac catgcgggag ctgtccaaag
     1321 agatgatgaa ggctggaatc atagaggaga tgttagagga tacatttgaa agcatggatg
     1381 atcaagaaga aatggaagaa gcagctgaga tggagattga cagaatcctc tttgaaatca
     1441 cagcaggagc cttgggcaaa gcacccagta aagtaaccga tgcccttcct gagcctgaac
     1501 ccgcaggagc gatggctgcc tccgaagagg gagaagagga agaagatgaa gaggacctgg
     1561 aggctatgca gtcacggctg gccacactca gaagctaccc atacgatgtt ccagattacg
     1621 cttgaaagct tgctagcaga tctttttccc tctgccaaaa attatgggga catcatgaag
     1681 ccccttgagc acttaagttt aaaccgctga tcagcctcga ctgtgccttc tagttgccag
     1741 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     1801 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     1861 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     1921 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     1981 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2041 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2101 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2161 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2221 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2281 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2341 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2401 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     2461 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     2521 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2581 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     2641 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     2701 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     2761 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     2821 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     2881 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     2941 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     3001 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     3061 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     3121 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     3181 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     3241 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     3301 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     3361 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     3421 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     3481 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     3541 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     3601 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     3661 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     3721 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     3781 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3841 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3901 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     3961 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4021 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4081 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4141 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4201 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4261 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4321 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4381 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4441 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4501 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4561 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4621 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4681 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4741 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4801 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4861 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4921 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4981 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5041 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5101 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5161 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5221 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     5281 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5341 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5401 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5461 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5521 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5581 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5641 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5701 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5761 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5821 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5881 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5941 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6001 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6061 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6121 cgtc
//
