LOCUS       Exported                4782 bp ds-DNA     circular SYN 26-MAY-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Path-IT
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 26, 2020 from SnapGene 5.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     promoter        36..1217
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human 
                     elongationfactor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          266..1208
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of 
                     humanEF-1-alpha"
                     /note="color: #------"
     CDS             1250..1690
                     /codon_start=1
                     /label=RetroCHMP3
                     /note="color: #ff0000"
                     /translation="MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEEVK
                     RSVKDEAKKGQKEVCVVLAKETIRSRKTVSKLYASKAHMNSVLMGMKNQLAVLRVAGSL
                     QKSTEVMKAMQSLVKILEIQATMRELSKEMMKAGIIKDVRGYI"
     CDS             1691..1717
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     polyA_site      1871..1926
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(2285..2301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    2309..2325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator 
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(2333..2363)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 2333 .. 2339 / #ffffff / -10
                        2: 2340 .. 2357 / #ffffff
                        3: 2358 .. 2363 / #ffffff / -35"
     protein_bind    2378..2399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the 
                     presenceof cAMP."
                     /note="color: #31849b"
     promoter        2458..2653
                     /label=SV40 promoter
                     /note="SV40 early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      2504..2639
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     polyA_signal    2659..2793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(3032..3620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(3791..4651)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3791 .. 4582 / #ccffcc
                        2: 4583 .. 4651 / #ccffcc / signal sequence
                     Cleavage site after base 4582"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4652..4756)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agatcatcgc gtgaggctcc ggtgcccgtc agtgggcaga
       61 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
      121 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      181 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      241 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
      301 ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg
      361 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
      421 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
      481 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
      541 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
      601 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
      661 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
      721 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
      781 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
      841 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
      901 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
      961 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     1021 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     1081 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     1141 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     1201 catttcaggt gtcgtgagga attctgcagt cgacggtacc caatttgcca tgggactgtt
     1261 tggaaaaacc caggagaagc ctcccaaaga gctggttaat gaatggtcac tgaagatcag
     1321 aaaggaaatg agagttgttg ataggcaaat aagagacatc caaagagaag aagaggaagt
     1381 aaaacggtct gtgaaagatg aagccaagaa gggccaaaag gaagtctgtg tggttctggc
     1441 caaggagacg atcaggtcaa ggaaaactgt gagcaagctc tatgcgtcca aagcacacat
     1501 gaactctgtg ctcatgggca tgaagaacca acttgcggtc ctgagagtag ctggttctct
     1561 gcagaagagc acagaagtga tgaaggcaat gcagagtctt gtgaagatcc tagaaatcca
     1621 ggccaccatg cgggagctgt ccaaagagat gatgaaagct ggaatcataa aggatgttag
     1681 aggatacatt tacccatacg atgttccaga ttacgcttga gcggccgcac tcctcaggtg
     1741 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac
     1801 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     1861 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     1921 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     1981 gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt ggctataaag
     2041 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     2101 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     2161 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     2221 catagctgtc cctcttctct tatgaagatc cctcgacctg cagcccaagc ttggcgtaat
     2281 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2341 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2401 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     2461 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     2521 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     2581 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     2641 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2701 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2761 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
     2821 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2881 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2941 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3001 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3061 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3121 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3181 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     3241 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3301 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3361 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3421 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     3481 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3541 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3601 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3661 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3721 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3781 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3841 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3901 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3961 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4021 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4081 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4141 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4201 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4261 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4321 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4381 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4441 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4501 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4561 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4621 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4681 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4741 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tg
//
