LOCUS       dCas9_VPR_P2A_mC       14796 bp    DNA        circular     20-MAY-2020
DEFINITION  .
ACCESSION   urn.local...s-687irqr
VERSION     urn.local...s-687irqr
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    10..20
                     /Recognition_pattern="CCNNNNN^NNGG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BslI"
                     /label="BslI"
                     /ApEinfo_label="BslI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    128..140
                     /Recognition_pattern="WCGNNNN^NNNCGW"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="PcsI"
                     /label="PcsI"
                     /ApEinfo_label="PcsI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(217..236)
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Pinco12"
                     /label="Pinco12"
                     /ApEinfo_label="Pinco12"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    289..299
                     /Recognition_pattern="CCNNNNN^NNGG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BslI(1)"
                     /label="BslI(1)"
                     /ApEinfo_label="BslI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(313..323)
                     /Recognition_pattern="CCANNNNNNGT(15/9)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjeI"
                     /label="CjeI"
                     /ApEinfo_label="CjeI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    343..354
                     /Recognition_pattern="CCANNNNNNNTC(14/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjePI"
                     /label="CjePI"
                     /ApEinfo_label="CjePI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(391..401)
                     /Recognition_pattern="CCANNNNNNGT(15/9)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjeI(1)"
                     /label="CjeI(1)"
                     /ApEinfo_label="CjeI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    392..402
                     /Recognition_pattern="CCANNNNNNGT(15/9)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjeI(2)"
                     /label="CjeI(2)"
                     /ApEinfo_label="CjeI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10397..11083
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff7d94"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    429..439
                     /Recognition_pattern="GCNNNNN^NNGC"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="MwoI"
                     /label="MwoI"
                     /ApEinfo_label="MwoI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_unit     complement(483..520)
                     /Description=" Tn3 inverted repeat ; Tn3 inverted repeat"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; ES-FUCCI (modified);
                     LMHT-MYCTETR; LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A;
                     RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     lentiCas9-Hygro; lentiCas9-U6-IT-;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="TN3_INV_RPT"
                     /label="TN3_INV_RPT"
                     /ApEinfo_label="TN3_INV_RPT"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    490..501
                     /Recognition_pattern="GACNNNNNNTGG(12/7)"
                     /annotation_group="GACNNNNNNTGG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; pCR4-TOPO
                     (modified); pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="Bsp24I"
                     /label="Bsp24I"
                     /ApEinfo_label="Bsp24I"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(490..501)
                     /Recognition_pattern="CCANNNNNNNTC(14/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjePI(1)"
                     /label="CjePI(1)"
                     /ApEinfo_label="CjePI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(491..501)
                     /Recognition_pattern="CCANNNNNNGT(15/9)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjeI(3)"
                     /label="CjeI(3)"
                     /ApEinfo_label="CjeI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    501..512
                     /Recognition_pattern="GAACNNNNNCTC(13/8)"
                     /annotation_group="GAACNNNNNCTC"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIKPB; RIKPB Copy;
                     RIKPH; SGEP-GFP-tTA-Linker; TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; pCR4-TOPO
                     (modified); pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="PpiI"
                     /label="PpiI"
                     /ApEinfo_label="PpiI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(610..621)
                     /Recognition_pattern="CCANNNNNNNTC(14/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjePI(2)"
                     /label="CjePI(2)"
                     /ApEinfo_label="CjePI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(630..1490)
                     /gene="Ampicillin"
                     /Transferred_Translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCS
                     DAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRD
                     WLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRM
                     EAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGF
                     IDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF
                     *"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Ampicillin"
                     /label="Ampicillin"
                     /ApEinfo_label="Ampicillin"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             complement(630..1148)
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 1 (frame 1)"
                     /label="ORF 1 (frame 1)"
                     /ApEinfo_label="ORF 1 (frame 1)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(698..708)
                     /Recognition_pattern="GAYNNNNNVTC(13/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="Hin4I"
                     /label="Hin4I"
                     /ApEinfo_label="Hin4I"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     759..778
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; ES-FUCCI (modified);
                     LMHT-MYCTETR; LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pCR4-TOPO (modified); pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pLVTHM (modified);
                     pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Pinco13"
                     /label="Pinco13"
                     /ApEinfo_label="Pinco13"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             760..1026
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /X-Transferred_From="TPGPB-Cas9(PX330);
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR)"
                     /X-Transferred_Similarity="99.63%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 1 (frame 2)"
                     /label="ORF 1 (frame 2)"
                     /ApEinfo_label="ORF 1 (frame 2)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    772..782
                     /Recognition_pattern="GAYNNNNNVTC(13/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="Hin4I(1)"
                     /label="Hin4I(1)"
                     /ApEinfo_label="Hin4I"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    951..960
                     /Recognition_pattern="CAYNN^NNRTG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="MslI"
                     /label="MslI"
                     /ApEinfo_label="MslI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1005..1017
                     /Recognition_pattern="WCGNNNN^NNNCGW"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="PcsI(1)"
                     /label="PcsI(1)"
                     /ApEinfo_label="PcsI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             1033..1203
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; SGEP-GFP-tTA-Linker; TPGPB-Cas9(PX330);
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR); VPR
                     sffv; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Hygro;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLVTHM (modified);
                     pLentiCRISPR-P2A-GFP vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 2 (frame 2)"
                     /label="ORF 2 (frame 2)"
                     /ApEinfo_label="ORF 2 (frame 2)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1082..1093)
                     /Recognition_pattern="CCANNNNNNNTC(14/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjePI(3)"
                     /label="CjePI(3)"
                     /ApEinfo_label="CjePI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1110..1119
                     /Recognition_pattern="CAYNN^NNRTG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="MslI(1)"
                     /label="MslI(1)"
                     /ApEinfo_label="MslI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1186..1196)
                     /Recognition_pattern="GACNNNNNTGA(13/11)"
                     /annotation_group="GACNNNNNTGA"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; pCR4-TOPO
                     (modified); pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="NgoAVIII"
                     /label="NgoAVIII"
                     /ApEinfo_label="NgoAVIII"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             1213..1362
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /X-Transferred_From="TPGPB-Cas9(PX330);
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR)"
                     /X-Transferred_Similarity="99.33%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 3 (frame 2)"
                     /label="ORF 3 (frame 2)"
                     /ApEinfo_label="ORF 3 (frame 2)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1219..1230
                     /Recognition_pattern="(10/12)CGANNNNNNTGC(12/10);
                     CGANNNNNNTGC(12/10)"
                     /annotation_group="CGANNNNNNTGC"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; Puro-P2A-tagBFP
                     reporter; RIKPB; RIKPB Copy; RIKPH; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9(PX330); TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; pCR4-TOPO
                     (modified); pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter
                     with desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BcgI"
                     /label="BcgI"
                     /ApEinfo_label="BcgI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             complement(1292..1393)
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; ES-FUCCI (modified);
                     LMHT-MYCTETR; LMHT-MYCTETRSNLS; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9(PX330); TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); VPR sffv; backup of
                     lentiCas9-Hygro; lentiCRISPR v2 (modified);
                     lentiCas9-Hygro; pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLVTHM
                     (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pLentiCRISPR-P2A-Thy1.1;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 60 (frame 1)"
                     /label="ORF 60 (frame 1)"
                     /ApEinfo_label="ORF 60 (frame 1)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1344..1355)
                     /Recognition_pattern="GAACNNNNNCTC(13/8)"
                     /annotation_group="GAACNNNNNCTC"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIKPB; RIKPB Copy;
                     RIKPH; SGEP-GFP-tTA-Linker; TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; pCR4-TOPO
                     (modified); pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="PpiI(1)"
                     /label="PpiI(1)"
                     /ApEinfo_label="PpiI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1469..1478
                     /Recognition_pattern="CAYNN^NNRTG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="MslI(2)"
                     /label="MslI(2)"
                     /ApEinfo_label="MslI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1531..1556
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; ES-FUCCI (modified);
                     RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of lentiCas9-Hygro;
                     lentiCas9-Hygro; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pLVTHM (modified);
                     pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promotor        complement(1532..1560)
                     /%_Identity=100
                     /Motif="ATTGTCTCATGAGCGGATACATATTTGAA"
                     /annotation_group="AMP promoter: 1 -> 29"
                     /modified_by="User"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; ES-FUCCI (modified);
                     LMHT-MYCTETR; LMHT-MYCTETRSNLS; RIEPTETronly;
                     RT3-turboGFP-miRE-neo 2PAC delta BbsI empty;
                     SGEP-GFP-tTA-Linker; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pSIN-T3G-hCD2-miRE-EF1a-Thy1.1;
                     pSIN-hU6-guideRNA-TRE3G-hCas9-P2A-Thy1.1 empty;
                     pSIN-hU6-guideRNA-TRE3G-hCas9-P2A-Thy1.1 empty Copy;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /%_Identity=100
                     /annotation_group="AMP promoter: 1 -> 29"
                     /modified_by="User"
                     /Transferred_From="amp promotor"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="AMP promoter"
                     /label="AMP promoter"
                     /ApEinfo_label="AMP promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1532..1570
                     /Description="Unknown feature type:Promoter"
                     /gene="Bacterial-Promoter prom"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; ES-FUCCI (modified);
                     LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiGuide-Puro (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo; pSIN-reporter
                     with desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Bacterial-Promoter prom"
                     /label="Bacterial-Promoter prom"
                     /ApEinfo_label="Bacterial-Promoter prom"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1584..1603
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Pinco14"
                     /label="Pinco14"
                     /ApEinfo_label="Pinco14"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1615..1626)
                     /Recognition_pattern="GACNNNNNNTGG(12/7)"
                     /annotation_group="GACNNNNNNTGG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pSIN-reporter with
                     desttagBFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="Bsp24I(1)"
                     /label="Bsp24I(1)"
                     /ApEinfo_label="Bsp24I"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1615..1626
                     /Recognition_pattern="CCANNNNNNNTC(14/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pSIN-reporter with
                     desttagBFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjePI(4)"
                     /label="CjePI(4)"
                     /ApEinfo_label="CjePI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1615..1625
                     /Recognition_pattern="CCANNNNNNGT(15/9)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pSIN-reporter with
                     desttagBFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjeI(4)"
                     /label="CjeI(4)"
                     /ApEinfo_label="CjeI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1862..2438
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="CMV_immearly_promoter"
                     /label="CMV_immearly_promoter"
                     /ApEinfo_label="CMV_immearly_promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             complement(1913..2023)
                     /Genetic_code="Standard"
                     /Reading_frame=3
                     /created_by="ORF Finder"
                     /X-Transferred_From="Clipboard Contents;
                     TPGPB-Cas9(PX330); TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Hygro;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-GFP
                     vol.2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 57 (frame 3)"
                     /label="ORF 57 (frame 3)"
                     /ApEinfo_label="ORF 57 (frame 3)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     enhancer        1937..2240
                     /Description="CMV enhancer human cytomegalovirus immediate
                     early enhancer"
                     /X-Transferred_From="pSIN-reporter with desttagBFP"
                     /X-Transferred_Similarity="99.67%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Enhancer_1"
                     /label="Enhancer_1"
                     /ApEinfo_label="Enhancer_1"
                     /ApEinfo_fwdcolor="#5ac3fa"
                     /ApEinfo_revcolor="#5ac3fa"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1941..2228
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="CAG_enhancer"
                     /label="CAG_enhancer"
                     /ApEinfo_label="CAG_enhancer"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2111..2228
                     /X-Transferred_From="LUPG-Cas9(PX335)-partial U6"
                     /X-Transferred_Similarity="99.15%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="CAG_enhancer(1)"
                     /label="CAG_enhancer(1)"
                     /ApEinfo_label="CAG_enhancer"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             2112..2287
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /X-Transferred_From="TPGPB-Cas9(PX330);
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR)"
                     /X-Transferred_Similarity="99.44%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 5 (frame 2)"
                     /label="ORF 5 (frame 2)"
                     /ApEinfo_label="ORF 5 (frame 2)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             2140..2279
                     /Genetic_code="Standard"
                     /Reading_frame=3
                     /created_by="ORF Finder"
                     /X-Transferred_From="TPGPB-Cas9(PX330);
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR)"
                     /X-Transferred_Similarity="99.29%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 6 (frame 3)"
                     /label="ORF 6 (frame 3)"
                     /ApEinfo_label="ORF 6 (frame 3)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             complement(2213..2317)
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /X-Transferred_From="Clipboard Contents;
                     TPGPB-Cas9(PX330); TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Hygro;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-GFP
                     vol.2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 56 (frame 2)"
                     /label="ORF 56 (frame 2)"
                     /ApEinfo_label="ORF 56 (frame 2)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2264..2275
                     /Recognition_pattern="CAANNNNNGTGG(12/10)"
                     /annotation_group="CAANNNNNGTGG"
                     /X-Transferred_From="pCMVR8.74 D64V; pSIN-reporter with
                     desttagBFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CspCI"
                     /label="CspCI"
                     /ApEinfo_label="CspCI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             2266..2394
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /X-Transferred_From="Clipboard Contents;
                     TPGPB-Cas9(PX330); TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Hygro;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-GFP
                     vol.2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 7 (frame 1)"
                     /label="ORF 7 (frame 1)"
                     /ApEinfo_label="ORF 7 (frame 1)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2458..2640
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5'LTR"
                     /label="5'LTR"
                     /ApEinfo_label="5'LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2458..2638
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLET*;
                     MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
                     QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATW
                     ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPVSPRRPD
                     *;
                     MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESF
                     RPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMT
                     VRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPND
                     ERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADK
                     SGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; backup of
                     lentiCas9-Hygro; lentiCRISPR 13450; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiCrispr mCherryP2AneoR; lentiCrispr
                     mCherryP2AneoR-improved tracer; pCW-Cas9 (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_5_LTR; HIV-1_5_LTR; HIV-1_5_LTR;
                     HIV-1_5_LTR; HIV-1_5_LTR; HIV-1_5_LTR; HIV-1_5_LTR;
                     HIV-1_5_L"
                     /label="HIV-1_5_LTR; HIV-1_5_LTR; HIV-1_5_LTR;
                     HIV-1_5_LTR; HIV-1_5_LTR; HIV-1_5_LTR; HIV-1_5_LTR;
                     HIV-1_5_L"
                     /ApEinfo_label="HIV-1_5_LTR; HIV-1_5_LTR; HIV-1_5_LTR;
                     HIV-1_5_LTR; HIV-1_5_LTR; HIV-1_5_LTR; HIV-1_5_LTR;
                     HIV-1_5_L"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2555..2639
                     /gene="U5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U5"
                     /label="U5"
                     /ApEinfo_label="U5"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2640..2657
                     /gene="PBS"
                     /SECDrawAs="Region"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiGuide-Puro (modified); pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="PBS"
                     /label="PBS"
                     /ApEinfo_label="PBS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2689..2826
                     /Description="Unknown feature type:Regulatory_Seq"
                     /gene="PSI(HIV) reg"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiGuide-Puro (modified); pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Psi+; psi"
                     /label="Psi+; psi"
                     /ApEinfo_label="Psi+; psi"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2701..2816
                     /gene="HIV-1 pack"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1 pack"
                     /label="HIV-1 pack"
                     /ApEinfo_label="HIV-1 pack"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2749..2793
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGASLIKHW*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; backup of
                     lentiCas9-Hygro; lentiCRISPR 13450; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiCrispr mCherryP2AneoR; lentiCrispr
                     mCherryP2AneoR-improved tracer; pCW-Cas9 (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_psi_pack"
                     /label="HIV-1_psi_pack"
                     /ApEinfo_label="HIV-1_psi_pack"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2794..3147
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Gag"
                     /label="Gag"
                     /ApEinfo_label="Gag"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2844..2853
                     /Recognition_pattern="GAANN^NNTTC"
                     /annotation_group="GAANNNNTTC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIKPB; RIKPB
                     Copy; RIKPH; SGEP-GFP-tTA-Linker; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="XmnI, Asp700I, MroXI, PdmI"
                     /label="XmnI, Asp700I, MroXI, PdmI"
                     /ApEinfo_label="XmnI, Asp700I, MroXI, PdmI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3208..3223
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_int"
                     /label="SV40_int"
                     /ApEinfo_label="SV40_int"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3297..3595
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RRE"
                     /label="RRE"
                     /ApEinfo_label="RRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3314..3518
                     /Description="Rev responsive element; Unknown feature
                     type:Regulatory_Seq"
                     /gene="RRE(HIV) reg"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Hygro; pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RRE; RRE(HIV) reg"
                     /label="RRE; RRE(HIV) reg"
                     /ApEinfo_label="RRE; RRE(HIV) reg"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3797..3810
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_int(1)"
                     /label="SV40_int(1)"
                     /ApEinfo_label="SV40_int"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4040..4062
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiGuide-Puro (modified); pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HRP"
                     /label="HRP"
                     /ApEinfo_label="HRP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4060..4183
                     /gene="PPT"
                     /SECDrawAs="Region"
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="PPT"
                     /label="PPT"
                     /ApEinfo_label="PPT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4063..4076
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro; lentiGuide-Puro (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="cPPT"
                     /label="cPPT"
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4064..4187
                     /Description="central polypurine tract (cPPT) and the
                     central termination sequence (CTS)"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Hygro; lentiGuide-Puro (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="cPPT; cPPT/CTS"
                     /label="cPPT; cPPT/CTS"
                     /ApEinfo_label="cPPT; cPPT/CTS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4067..4088
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U3PPT"
                     /label="U3PPT"
                     /ApEinfo_label="U3PPT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4067..4082
                     /X-Transferred_Translation="MEWDREINNYTSLIHSLIEESQNQQEKNEQ
                     ELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLS
                     FQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSRRYRQMAVFIHNFKRKGGIGGY
                     SAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRDPVWIDKLDIEFLQPR*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRIDTVDLEGINSS
                     SVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDLLFACTGSLWLDQI*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRIDTVDLET*;
                     MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
                     QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATW
                     ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPVSPRRPD
                     *;
                     MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGA
                     ASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLKDQ
                     QLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDREINNYTSLIHSLIEE
                     SQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFAVLSIVN
                     RVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSALRAPILQTNGS
                     IHPQF*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; backup of
                     lentiCas9-Hygro; lentiCRISPR 13450; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiCrispr mCherryP2AneoR; lentiCrispr
                     mCherryP2AneoR-improved tracer; pCW-Cas9 (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="cPPT(1)"
                     /label="cPPT(1)"
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4248..4498
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="EFIas; EFIas; EFS; EFS; EFS"
                     /label="EFIas; EFIas; EFS; EFS; EFS"
                     /ApEinfo_label="EFIas; EFIas; EFS; EFS; EFS"
                     /ApEinfo_fwdcolor="#bc8dbf"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             4508..8647
                     /X-Transferred_Translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSK
                     KFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSN
                     EMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDST
                     DKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINAS
                     GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDA
                     KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSAS
                     MIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKP
                     ILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDN
                     REKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIER
                     MTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL
                     FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNE
                     ENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLIN
                     GIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIAN
                     LAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRI
                     EEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIV
                     PQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL
                     TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITL
                     KSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVY
                     DVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVW
                     DKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYG
                     GFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEV
                     KKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGS
                     PEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQA
                     ENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQ
                     LGGDSRADPKKKRKV*"
                     /X-Transferred_From="pAdSh.PGK.Cas9 (modified)"
                     /X-Transferred_Similarity="99.81%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF frame 1"
                     /label="ORF frame 1"
                     /ApEinfo_label="ORF frame 1"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             complement(5912..6430)
                     /Transferred_Translation="MSVSFQAFFNHLPIFKQRKGEDNVLKNVLVLL
                     IVQEVLVFNDFQEIVIRSQGCVEAILHSADFNRVETFNLFEIVFFELFHGNFPVRLEE
                     EVHDSFLLLSRQECWLSHPFCDVFDLGELVINCEVLVQQRVFRKHLFVRQIFIKVSHP
                     FDEGLGRGPLIHDFLEVPGSDGLF*"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF frame 1(1)"
                     /label="ORF frame 1(1)"
                     /ApEinfo_label="ORF frame 1"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6049..6061)
                     /Recognition_pattern="GAAGNNNNNNTAC(12/7)"
                     /annotation_group="GAAGNNNNNNTAC"
                     /X-Transferred_From="TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; Vol2
                     pSGRNAUITEC9PG; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BarI"
                     /label="BarI"
                     /ApEinfo_label="BarI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7159..7169
                     /Recognition_pattern="GCANNNNN^TGC; GCANNNN^NTGC"
                     /annotation_group="GCANNNNNTGC"
                     /X-Transferred_From="Clipboard Contents; Vol2
                     pSGRNAUITEC9PG; pCRISPR-6xTET-sffv-CAS9-P2A-GFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="ApaBI; ApaBI; BstAPI; BstAPI"
                     /label="ApaBI; ApaBI; BstAPI; BstAPI"
                     /ApEinfo_label="ApaBI; ApaBI; BstAPI; BstAPI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7636..7645
                     /Recognition_pattern="GAANN^NNTTC"
                     /annotation_group="GAANNNNTTC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIKPB; RIKPB
                     Copy; RIKPH; SGEP-GFP-tTA-Linker; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="XmnI, Asp700I, MroXI, PdmI(1)"
                     /label="XmnI, Asp700I, MroXI, PdmI(1)"
                     /ApEinfo_label="XmnI, Asp700I, MroXI, PdmI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             complement(8021..8554)
                     /Transferred_Translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQ
                     VGQSKQVDNVFCLLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLF
                     HEQLLLLIIFGRPFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKER
                     VLGKLNDEVFFDLFISFRLEKVDGVFFEA*"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF frame 1(2)"
                     /label="ORF frame 1(2)"
                     /ApEinfo_label="ORF frame 1"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8624..8644
                     /Transferred_Translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQ
                     VGQSKQVDNVFCLLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLF
                     HEQLLLLIIFGRPFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKER
                     VLGKLNDEVFFDLFISFRLEKVDGVFFEA*"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8681..8705
                     /Transferred_Translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQ
                     VGQSKQVDNVFCLLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLF
                     HEQLLLLIIFGRPFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKER
                     VLGKLNDEVFFDLFISFRLEKVDGVFFEA*"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="attB1"
                     /label="attB1"
                     /ApEinfo_label="attB1"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(8692..8701)
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     lentiCrispr mCherryP2AneoR; pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro, lentiCRISPR
                     v2 (modified), lentiCrispr mCherryP2AneoR,
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2;
                     LentiCrisprmCherryP2Apuro; Vol2 pSGRNAUITEC9PG;
                     lentiCas9-U6-IT-; lentiCrispr mCherryP2AneoR;
                     pLentiCRISPR-P2A-Thy1.1; pRRL-PX335-P2A-GFP Delta BbsI"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U6 terminator"
                     /label="U6 terminator"
                     /ApEinfo_label="U6 terminator"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     active_site     8711..8896
                     /created_by="User"
                     /X-Transferred_From="Clipboard Contents 6; M-ST1-VPR
                     (modified); VPR sffv"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="VP64"
                     /label="VP64"
                     /ApEinfo_label="VP64"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8909..8932
                     /Transferred_Translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQ
                     VGQSKQVDNVFCLLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLF
                     HEQLLLLIIFGRPFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKER
                     VLGKLNDEVFFDLFISFRLEKVDGVFFEA*"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 NLS(1)"
                     /label="SV40 NLS(1)"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     active_site     8933..9715
                     /created_by="User"
                     /X-Transferred_From="M-ST1-VPR (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="P65"
                     /label="P65"
                     /ApEinfo_label="P65"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9502..9512
                     /Recognition_pattern="GCANNNNN^TGC; GCANNNN^NTGC"
                     /annotation_group="GCANNNNNTGC"
                     /X-Transferred_From="Clipboard Contents; Vol2
                     pSGRNAUITEC9PG; pCRISPR-6xTET-sffv-CAS9-P2A-GFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="ApaBI; ApaBI; BstAPI; BstAPI(1)"
                     /label="ApaBI; ApaBI; BstAPI; BstAPI(1)"
                     /ApEinfo_label="ApaBI; ApaBI; BstAPI; BstAPI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     active_site     9733..10303
                     /created_by="User"
                     /X-Transferred_From="Clipboard Contents 6; M-ST1-VPR
                     (modified); VPR sffv"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RTA"
                     /label="RTA"
                     /ApEinfo_label="RTA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     DNA_probe_bind  complement(10278..10317)
                     /Sequence="CCGCTGCCGGATCCAAACAGAGATGTGTCGAAGATGGACA"
                     /created_by="User"
                     /locus_tag="zz"
                     /label="zz"
                     /ApEinfo_label="zz"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10310..10375
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; pLentiCRISPR;
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#f6989d"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    11111..11699
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro; lentiGuide-Puro (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pLentiCRISPR;
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#bc8dbf"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11887..11908
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDLLFACTGSLWLDQI*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRIDTVDLET*;
                     MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
                     QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATW
                     ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPVSPRRPD
                     *"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; backup of
                     lentiCas9-Hygro; lentiCRISPR 13450; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiCrispr mCherryP2AneoR; lentiCrispr
                     mCherryP2AneoR-improved tracer; pCW-Cas9 (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U3PPT(1)"
                     /label="U3PPT(1)"
                     /ApEinfo_label="U3PPT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11903..11956
                     /invitrogen=1410000
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Blast (modified); lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="delta U3"
                     /label="delta U3"
                     /ApEinfo_label="delta U3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             12170..12405
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Blast (modified); lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="3' LTR SIN"
                     /label="3' LTR SIN"
                     /ApEinfo_label="3' LTR SIN"
                     /ApEinfo_fwdcolor="#8882be"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             12224..12405
                     /gene="RU5"
                     /SECDrawAs="Region"
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RU5"
                     /label="RU5"
                     /ApEinfo_label="RU5"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12224..12406
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5'LTR(1)"
                     /label="5'LTR(1)"
                     /ApEinfo_label="5'LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12224..12404
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLET*;
                     MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
                     QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATW
                     ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPVSPRRPD
                     *;
                     MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESF
                     RPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMT
                     VRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPND
                     ERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADK
                     SGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; backup of
                     lentiCas9-Hygro; lentiCRISPR 13450; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiCrispr mCherryP2AneoR; lentiCrispr
                     mCherryP2AneoR-improved tracer; pCW-Cas9 (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_5_LTR"
                     /label="HIV-1_5_LTR"
                     /ApEinfo_label="HIV-1_5_LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12224..12322
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="R"
                     /label="R"
                     /ApEinfo_label="R"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12321..12405
                     /gene="U5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U5(1)"
                     /label="U5(1)"
                     /ApEinfo_label="U5"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             12412..12643
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="bGH polyA"
                     /label="bGH polyA"
                     /ApEinfo_label="bGH polyA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      12433..12660
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLET*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRVDFKTNDLQGSC
                     RS*"
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="bGH_PA_terminator"
                     /label="bGH_PA_terminator"
                     /ApEinfo_label="bGH_PA_terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      12706..13161
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#f49ac2"
                     /ApEinfo_revcolor="#f49ac2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12879..13037
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified)"
                     /X-Transferred_Similarity="99.37%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SQ10"
                     /label="SQ10"
                     /ApEinfo_label="SQ10"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12977..12988
                     /Recognition_pattern="GACNNNN^NNGTC"
                     /annotation_group="GACNNNNNNGTC"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIKPB; RIKPB
                     Copy; RIKPH; SGEP-GFP-tTA-Linker; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="DrdI, AasI, DseDI"
                     /label="DrdI, AasI, DseDI"
                     /ApEinfo_label="DrdI, AasI, DseDI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(12983..12995)
                     /Recognition_pattern="(7/12)GAACNNNNNNTCC(12/7);
                     GAACNNNNNNTCC(12/7)"
                     /annotation_group="GAACNNNNNNTCC"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIKPB; RIKPB
                     Copy; RIKPH; SGEP-GFP-tTA-Linker; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="AloI"
                     /label="AloI"
                     /ApEinfo_label="AloI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    13130..13139
                     /Recognition_pattern="GAANN^NNTTC"
                     /X-Transferred_From="LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="XmnI"
                     /label="XmnI"
                     /ApEinfo_label="XmnI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(13149..13364)
                     /X-Transferred_From="TIGPB-Cas9(PX330); TIGPN-Cas9(PX330);
                     TPGPB-Cas9(PX330); backup of lentiCas9-Hygro; lentiCRISPR
                     v2 (modified); lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_Translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQ
                     VGQSKQVDNVFCLLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLF
                     HEQLLLLIIFGRPFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKER
                     VLGKLNDEVFFDLFISFRLEKVDGVFFEA*"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_enhancer"
                     /label="SV40_enhancer"
                     /ApEinfo_label="SV40_enhancer"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    13161..13429
                     /X-Transferred_From="Puro-P2A-tagBFP reporter; TIGPB;
                     TIGPB-Cas9(PX330); TIGPN; TIGPN-Cas9(PX330);
                     TPGPB-Cas9(PX330); TPGPB-Cas9lentiCRISPR);
                     TPGPB-ERT1Cas9ERT2noNLS; TPGPB-ERTCas9; TPGPB-ERTCas9ERT;
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     pLVTHM (modified)"
                     /X-Transferred_Similarity="99.63%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="SV40_promoter"
                     /label="SV40_promoter"
                     /ApEinfo_label="SV40_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    13161..13212
                     /Description="Unknown feature type:Promoter"
                     /gene="SV40 prom"
                     /X-Transferred_From="TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pSIN-reporter with
                     desttagBFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 Promoter (destroyed); SV40 prom"
                     /label="SV40 Promoter (destroyed); SV40 prom"
                     /ApEinfo_label="SV40 Promoter (destroyed); SV40 prom"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    13185..13207
                     /X-Transferred_From="TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pSIN-reporter with
                     desttagBFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 Rev; SV40seqRev"
                     /label="SV40 Rev; SV40seqRev"
                     /ApEinfo_label="SV40 Rev; SV40seqRev"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    13233..13284
                     /Description="Unknown feature type:Promoter"
                     /gene="SV40 prom"
                     /X-Transferred_From="TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pSIN-reporter with
                     desttagBFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 Promoter (destroyed); SV40 prom(1)"
                     /label="SV40 Promoter (destroyed); SV40 prom(1)"
                     /ApEinfo_label="SV40 Promoter (destroyed); SV40 prom"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    13257..13279
                     /X-Transferred_From="TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pSIN-reporter with
                     desttagBFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 Rev; SV40seqRev(1)"
                     /label="SV40 Rev; SV40seqRev(1)"
                     /ApEinfo_label="SV40 Rev; SV40seqRev"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    13308..13371
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer;
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9GFPERT2; backup
                     of lentiCas9-Hygro; lentiCRISPR v2 (modified);
                     lentiCas9-Hygro; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pLVTHM (modified);
                     pLentiCRISPR-P2A-GFP vol.2; pLentiCRISPR-P2A-Thy1.1;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 3x21bp repeat"
                     /label="SV40 3x21bp repeat"
                     /ApEinfo_label="SV40 3x21bp repeat"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      13328..13463
                     /note="SV40 ori; SV40 origin of replication"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="99.26%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 ori rep origin"
                     /label="SV40 ori rep origin"
                     /ApEinfo_label="SV40 ori rep origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      13328..13405
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRVDFKTNDLQGSCRS*"
                     /X-Transferred_From="TIGPB-Cas9(PX330); TIGPN-Cas9(PX330);
                     TPGPB-Cas9(PX330); backup of lentiCas9-Hygro; lentiCRISPR
                     v2 (modified); lentiCas9-Hygro; lentiGuide-Puro
                     (modified); pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentisgRNA Thy1.1-P2A-neo +Kosak; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak - improved backbone andkilled BsmBi
                     site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_Translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQ
                     VGQSKQVDNVFCLLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLF
                     HEQLLLLIIFGRPFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKER
                     VLGKLNDEVFFDLFISFRLEKVDGVFFEA*"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_origin"
                     /label="SV40_origin"
                     /ApEinfo_label="SV40_origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    13406..13416
                     /Recognition_pattern="(8/13)GAGNNNNNCTC(13/8);
                     GAGNNNNNCTC(13/-24)"
                     /annotation_group="GAGNNNNNCTC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; Puro-P2A-tagBFP
                     reporter; RIKPB; RIKPB Copy; RIKPH; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9(PX330); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter
                     with desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BplI"
                     /label="BplI"
                     /ApEinfo_label="BplI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        13523..13590
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLET*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRVDFKTNDLQGSC
                     RS*"
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="EM7_promoter"
                     /label="EM7_promoter"
                     /ApEinfo_label="EM7_promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            13591..13962
                     /gene="bleo"
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLET*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRVDFKTNDLQGSC
                     RS*"
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="bleo"
                     /label="bleo"
                     /ApEinfo_label="bleo"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     14095..14225
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; VPR sffv; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 polyA"
                     /label="SV40 polyA"
                     /ApEinfo_label="SV40 polyA"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      14098..14229
                     /X-Transferred_From="Clipboard Contents; backup of
                     lentiCas9-Hygro; lentiCRISPR v2 (modified);
                     lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-GFP
                     vol.2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_PA_terminator"
                     /label="SV40_PA_terminator"
                     /ApEinfo_label="SV40_PA_terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14255..14484
                     /Description="lac operon sequence"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Misc_Feature_4"
                     /label="Misc_Feature_4"
                     /ApEinfo_label="Misc_Feature_4"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(14261..14279)
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiGuide-Puro (modified); pCR4-TOPO (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-GFP
                     vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="M13_reverse_primer"
                     /label="M13_reverse_primer"
                     /ApEinfo_label="M13_reverse_primer"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(14278..14300)
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLET*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRVDFKTNDLQGSC
                     RS*;
                     MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESF
                     RPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMT
                     VRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPND
                     ERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADK
                     SGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; backup of
                     lentiCas9-Hygro; lentiCRISPR 13450; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiCrispr mCherryP2AneoR; lentiCrispr
                     mCherryP2AneoR-improved tracer; lentiGuide-Puro
                     (modified); pCW-Cas9 (modified); pLentiCRISPR-P2A-Thy1.1;
                     pLentisgRNA Thy1.1-P2A-neo +Kosak; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak - improved backbone andkilled BsmBi
                     site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_Translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCS
                     DAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRD
                     WLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRM
                     EAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGF
                     IDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF
                     *"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="M13_pUC_rev_primer"
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_label="M13_pUC_rev_primer"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(14287..14307)
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiGuide-Puro (modified); pCR4-TOPO (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-GFP
                     vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="LacO(1)"
                     /label="LacO(1)"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(14289..14305)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).; lac
                     operator"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="lac operator protein bind"
                     /label="lac operator protein bind"
                     /ApEinfo_label="lac operator protein bind"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14289..14305
                     /Description="lac operator"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LentiCrisprmCherryP2Apuro-improved
                     tracer; RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiGuide-Puro (modified); pCR4-TOPO (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-GFP
                     vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Misc_Feature_5"
                     /label="Misc_Feature_5"
                     /ApEinfo_label="Misc_Feature_5"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(14313..14343)
                     /note="lac promoter; promoter for the E. coli lac operon"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14344..14354
                     /Recognition_pattern="GCNNNNN^NNGC"
                     /X-Transferred_From="LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="MwoI(1)"
                     /label="MwoI(1)"
                     /ApEinfo_label="MwoI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(14358..14379)
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP.; CAP binding site"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="CAP binding site protein bind"
                     /label="CAP binding site protein bind"
                     /ApEinfo_label="CAP binding site protein bind"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14363..14374
                     /Recognition_pattern="TGANNNNNNTCA(12/-24)"
                     /annotation_group="TGANNNNNNTCA"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     SGEP-GFP-tTA-Linker; VPR sffv; pCR4-TOPO (modified);
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BdaI"
                     /label="BdaI"
                     /ApEinfo_label="BdaI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14388..14398
                     /Recognition_pattern="GCNNNNN^NNGC"
                     /X-Transferred_From="LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="MwoI(2)"
                     /label="MwoI(2)"
                     /ApEinfo_label="MwoI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14448..14458
                     /Recognition_pattern="CCNNNNN^NNGG"
                     /X-Transferred_From="LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BslI(2)"
                     /label="BslI(2)"
                     /ApEinfo_label="BslI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(14459..14469)
                     /Recognition_pattern="ACNNNNNCTCC(10/7)"
                     /annotation_group="ACNNNNNCTCC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     SGEP-GFP-tTA-Linker; VPR sffv; pCR4-TOPO (modified);
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BsaXI"
                     /label="BsaXI"
                     /ApEinfo_label="BsaXI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14472..14482
                     /Recognition_pattern="GCNNNNN^NNGC"
                     /X-Transferred_From="LMHT-MYCTETR; LMHT-MYCTETRSNLS;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="MwoI(3)"
                     /label="MwoI(3)"
                     /ApEinfo_label="MwoI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(14483..14497)
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; RIEPTETronly; SGEP-GFP-tTA-Linker;
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiCas9-U6-IT-; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pLVTHM (modified);
                     pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_Translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKV
                     GIHGVPAADKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL
                     LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEE
                     DKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGH
                     FLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENL
                     IAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIG
                     DQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
                     GNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLY
                     EYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE
                     CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREM
                     IEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFA
                     NRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDEL
                     VKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQ
                     LQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN
                     RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQL
                     VETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREIN
                     NYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYF
                     FYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIV
                     KKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGK
                     SKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRK
                     RMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEI
                     IEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYF
                     DTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAGQAKKKK*
                     /X-Transferred_Translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKR
                     KVGIHGVPAADKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIG
                     ALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLV
                     EEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFR
                     GHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALV
                     RQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNRED
                     LLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPL
                     ARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSL
                     LYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKK
                     IECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDR
                     EMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDG
                     FANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD
                     ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVEN
                     TQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSD
                     KNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKR
                     QLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVRE
                     INNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAK
                     YFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVN
                     IVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEK
                     GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENG
                     RKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFK
                     YFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAGQAKKK
                     K*"
                     /X-Transferred_From="lentiCRISPR 13450; pCW-Cas9
                     (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR 13450, pCW-Cas9
                     (modified),pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="80.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(14484..14547)
                     /Description=" L-strand Y effector site ; L-strand Y
                     effector site; L-strand Y effector site "
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     lentiCas9-Hygro; lentiCas9-U6-IT-;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="L_Y_EFF"
                     /label="L_Y_EFF"
                     /ApEinfo_label="L_Y_EFF"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(14489..14500)
                     /product="3' stem, pBR pas(L)"
                     /gene="pas'"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     lentiCas9-Hygro; lentiCas9-U6-IT-;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo; pSIN-reporter
                     with desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="(pas')"
                     /label="(pas')"
                     /ApEinfo_label="(pas')"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14526..14538
                     /Recognition_pattern="WCGNNNN^NNNCGW"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="PcsI(2)"
                     /label="PcsI(2)"
                     /ApEinfo_label="PcsI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(14532..14542)
                     /product="5' stem, pBR primosome ass'y site (L)"
                     /gene="pas"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; ES-FUCCI (modified);
                     LMHT-MYCTETR; LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A;
                     RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     lentiCas9-Hygro; lentiCas9-U6-IT-;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo; pSIN-reporter
                     with desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="(pas)"
                     /label="(pas)"
                     /ApEinfo_label="(pas)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14539..14549
                     /Recognition_pattern="GCNNNNN^NNGC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="MwoI(4)"
                     /label="MwoI(4)"
                     /ApEinfo_label="MwoI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             complement(14584..14697)
                     /Genetic_code="Standard"
                     /Reading_frame=3
                     /created_by="ORF Finder"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; RIKPB; RIKPB Copy; RIKPH;
                     SGEP-GFP-tTA-Linker; TPGPB-Cas9(PX330);
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR); VPR
                     sffv; backup of lentiCas9-Hygro; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCRISPR v2
                     (modified); lentiCas9-Hygro;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLVTHM (modified);
                     pLentiCRISPR-P2A-GFP vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified),
                     pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 42 (frame 3)"
                     /label="ORF 42 (frame 3)"
                     /ApEinfo_label="ORF 42 (frame 3)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(14608..14796,1..485)
                     /Description="derived originally from a ColE1-like
                     plasmid, pMB1"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(join(14614..14796,1..38))
                     /Description="putative"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; VPR sffv; pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="PMB1"
                     /label="PMB1"
                     /ApEinfo_label="PMB1"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14622..14632
                     /Recognition_pattern="CCNNNNN^NNGG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BslI(3)"
                     /label="BslI(3)"
                     /ApEinfo_label="BslI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14633..14643
                     /Recognition_pattern="CCANNNNNNGT(15/9)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CjeI(5)"
                     /label="CjeI(5)"
                     /ApEinfo_label="CjeI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14640..14650
                     /Recognition_pattern="CCNNNNN^NNGG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BslI(4)"
                     /label="BslI(4)"
                     /ApEinfo_label="BslI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(14652..14796,1..475)
                     /Description="Unknown feature type:Rep_Origin"
                     /gene="pUC origin"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="pUC origin"
                     /label="pUC origin"
                     /ApEinfo_label="pUC origin"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14653..14663
                     /Recognition_pattern="GCNNNNN^NNGC"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9lentiCRISPR); TPmCPB-Cas9(lentiCRISPR);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; Vol2 pSGRNAUITEC9PG; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="MwoI(5)"
                     /label="MwoI(5)"
                     /ApEinfo_label="MwoI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14688..14699
                     /Recognition_pattern="TGANNNNNNTCA(12/-24)"
                     /annotation_group="TGANNNNNNTCA"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIKPB; RIKPB Copy;
                     RIKPH; SGEP-GFP-tTA-Linker; TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; pCR4-TOPO
                     (modified); pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BdaI(1)"
                     /label="BdaI(1)"
                     /ApEinfo_label="BdaI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    14708..14719
                     /Recognition_pattern="GACNNNN^NNGTC"
                     /annotation_group="GACNNNNNNGTC"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; Puro-P2A-tagBFP
                     reporter; RIKPB; RIKPB Copy; RIKPH; SGEP-GFP-tTA-Linker;
                     TPGPB-Cas9(PX330); TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv; Vol2
                     pSGRNAUITEC9PG; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; pCR4-TOPO
                     (modified); pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter
                     with desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="SP-dCas9-VPR (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="DrdI; DrdI, AasI, DseDI; DrdI, AasI, DseDI;
                     DrdI, AasI, DseDI; DrdI, AasI, DseDI; DrdI; DrdI, AasI, D"
                     /label="DrdI; DrdI, AasI, DseDI; DrdI, AasI, DseDI; DrdI,
                     AasI, DseDI; DrdI, AasI, DseDI; DrdI; DrdI, AasI, D"
                     /ApEinfo_label="DrdI; DrdI, AasI, DseDI; DrdI, AasI,
                     DseDI; DrdI, AasI, DseDI; DrdI, AasI, DseDI; DrdI; DrdI,
                     AasI, D"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     orf             complement(join(14743..14796,1..66))
                     /Genetic_code="Standard"
                     /Reading_frame=3
                     /created_by="ORF Finder"
                     /X-Transferred_From="TPGPB-Cas9(PX330); backup of
                     lentiCas9-Hygro; lentiCRISPR v2 (modified);
                     lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR-P2A-GFP vol.2"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="ORF 41 (frame 3)"
                     /label="ORF 41 (frame 3)"
                     /ApEinfo_label="ORF 41 (frame 3)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10666..10666
                     /locus_tag="Silent SNP, A instead of G in seq"
                     /label="Silent SNP, A instead of G in seq"
                     /ApEinfo_label="Silent SNP, A instead of G in seq"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
       61 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      121 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      181 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      241 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      301 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      361 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      421 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      481 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      541 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      601 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      661 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      721 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      781 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      841 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      901 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      961 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1021 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1081 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1141 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1201 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1261 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1321 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1381 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1441 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1501 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1561 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1621 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1681 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1741 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1801 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     1861 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     1921 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     1981 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2041 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2101 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2161 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2221 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2281 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2341 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2401 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     2461 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2521 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2581 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2641 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     2701 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     2761 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     2821 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     2881 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     2941 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     3001 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     3061 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     3121 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     3181 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     3241 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     3301 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     3361 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     3421 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     3481 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     3541 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     3601 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     3661 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     3721 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     3781 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     3841 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     3901 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     3961 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     4021 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     4081 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     4141 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4201 cagagatcca gtttggttaa ttagctagct aggtcttgaa aggagtggga attggctccg
     4261 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     4321 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     4381 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     4441 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggaccgg ttctagagcg
     4501 ctccaccatg gacaagaagt actccattgg gctcgctatc ggcacaaaca gcgtcggctg
     4561 ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac
     4621 cgatcgccac agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac
     4681 ggccgaagcc acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg
     4741 gatctgctac ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt
     4801 ccataggctg gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat
     4861 ctttggcaat atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct
     4921 gaggaagaag cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct
     4981 ggcgcatatg atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa
     5041 cagcgatgtc gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga
     5101 gaacccgatc aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa
     5161 atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt
     5221 tggtaatctt atcgccctgt cactcgggct gacccccaac tttaaatcta acttcgacct
     5281 ggccgaagat gccaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct
     5341 gctggcccag atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga
     5401 cgccattctg ctgagtgata ttctgcgagt gaacacggag atcaccaaag ctccgctgag
     5461 cgctagtatg atcaagcgct atgatgagca ccaccaagac ttgactttgc tgaaggccct
     5521 tgtcagacag caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg
     5581 ctacgccgga tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc
     5641 catcttggaa aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct
     5701 gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc caccagattc acctgggcga
     5761 actgcacgct atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga
     5821 aaagattgag aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg
     5881 aaattccaga ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt
     5941 cgaggaagtc gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt
     6001 tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt
     6061 cacagtttat aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc
     6121 attcctgtct ggagagcaga agaaagctat cgtggacctc ctcttcaaga cgaaccggaa
     6181 agttaccgtg aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt
     6241 tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct
     6301 gaaaatcatt aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga
     6361 cattgtcctc acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac
     6421 ttacgctcat ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg
     6481 atgggggcgg ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac
     6541 aatcctggat tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca
     6601 tgatgactct ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga
     6661 cagtcttcac gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact
     6721 gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa
     6781 tatcgttatc gagatggccc gagagaacca aactacccag aagggacaga agaacagtag
     6841 ggaaaggatg aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga
     6901 acacccagtt gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa
     6961 cggcagggac atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt
     7021 ggctgctatc gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac
     7081 aagatccgat aaagctagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa
     7141 aatgaaaaat tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga
     7201 taatctgact aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa
     7261 aaggcagctt gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg
     7321 catgaacacc aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct
     7381 gaagtctaag ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat
     7441 caacaattac caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat
     7501 caaaaaatat cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt
     7561 taggaaaatg atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt
     7621 ttacagcaat attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg
     7681 gaagcgacca cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag
     7741 ggatttcgcg acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac
     7801 cgaagtacag accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa
     7861 gctgatcgca cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac
     7921 agtcgcttac agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa
     7981 aagcgtcaag gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc
     8041 catcgacttt ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct
     8101 tcccaagtac tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg
     8161 cgagctgcag aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct
     8221 ggccagccac tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt
     8281 cgtggaacaa cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa
     8341 aagagtgatc ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag
     8401 ggataagccc atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt
     8461 gggcgcgcct gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc
     8521 tacaaaggag gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac
     8581 aagaatcgac ctctctcagc tcggtggaga cagcagggct gaccccaaga agaagaggaa
     8641 ggtgtcgcca gggatccgtc gacttgacgc gttgatatca acaagtttgt acaaaaaagc
     8701 aggctacaaa gaggccagcg gttccggacg ggctgacgca ttggacgatt ttgatctgga
     8761 tatgctggga agtgacgccc tcgatgattt tgaccttgac atgcttggtt cggatgccct
     8821 tgatgacttt gacctcgaca tgctcggcag tgacgccctt gatgatttcg acctggacat
     8881 gctgattaac tctagaagtt ccggatctcc gaaaaagaaa cgcaaagttg gtagccagta
     8941 cctgcccgac accgacgacc ggcaccggat cgaggaaaag cggaagcgga cctacgagac
     9001 attcaagagc atcatgaaga agtccccctt cagcggcccc accgacccta gacctccacc
     9061 tagaagaatc gccgtgccca gcagatccag cgccagcgtg ccaaaacctg ccccccagcc
     9121 ttaccccttc accagcagcc tgagcaccat caactacgac gagttcccta ccatggtgtt
     9181 ccccagcggc cagatctctc aggcctctgc tctggctcca gcccctcctc aggtgctgcc
     9241 tcaggctcct gctcctgcac cagctccagc catggtgtct gcactggctc aggcaccagc
     9301 acccgtgcct gtgctggctc ctggacctcc acaggctgtg gctccaccag cccctaaacc
     9361 tacacaggcc ggcgagggca cactgtctga agctctgctg cagctgcagt tcgacgacga
     9421 ggatctggga gccctgctgg gaaacagcac cgatcctgcc gtgttcaccg acctggccag
     9481 cgtggacaac agcgagttcc agcagctgct gaaccagggc atccctgtgg cccctcacac
     9541 caccgagccc atgctgatgg aataccccga ggccatcacc cggctcgtga caggcgctca
     9601 gaggcctcct gatccagctc ctgcccctct gggagcacca ggcctgccta atggactgct
     9661 gtctggcgac gaggacttca gctctatcgc cgatatggat ttctcagcct tgctgggctc
     9721 tggcagcggc agccgggatt ccagggaagg gatgtttttg ccgaagcctg aggccggctc
     9781 cgctattagt gacgtgtttg agggccgcga ggtgtgccag ccaaaacgaa tccggccatt
     9841 tcatcctcca ggaagtccat gggccaaccg cccactcccc gccagcctcg caccaacacc
     9901 aaccggtcca gtacatgagc cagtcgggtc actgaccccg gcaccagtcc ctcagccact
     9961 ggatccagcg cccgcagtga ctcccgaggc cagtcacctg ttggaggatc ccgatgaaga
    10021 gacgagccag gctgtcaaag cccttcggga gatggccgat actgtgattc cccagaagga
    10081 agaggctgca atctgtggcc aaatggacct ttcccatccg cccccaaggg gccatctgga
    10141 tgagctgaca accacacttg agtccatgac cgaggatctg aacctggact cacccctgac
    10201 cccggaattg aacgagattc tggatacctt cctgaacgac gagtgcctct tgcatgccat
    10261 gcatatcagc acaggactgt ccatcttcga cacatctctg tttggatccg gcagcggcgc
    10321 caccaacttc agcctgctga agcaggccgg cgacgtggag gagaaccccg gccccatggt
    10381 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
    10441 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
    10501 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
    10561 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
    10621 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
    10681 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
    10741 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
    10801 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
    10861 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
    10921 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
    10981 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
    11041 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtgagaat tcgatatcaa
    11101 gcttatcggt aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
    11161 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
    11221 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
    11281 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
    11341 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
    11401 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
    11461 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
    11521 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
    11581 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
    11641 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
    11701 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
    11761 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
    11821 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
    11881 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
    11941 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
    12001 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
    12061 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
    12121 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
    12181 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
    12241 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    12301 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    12361 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
    12421 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
    12481 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
    12541 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
    12601 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
    12661 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
    12721 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    12781 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    12841 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    12901 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    12961 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    13021 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    13081 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
    13141 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    13201 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    13261 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    13321 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    13381 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
    13441 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
    13501 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
    13561 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
    13621 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
    13681 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
    13741 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
    13801 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
    13861 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
    13921 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
    13981 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    14041 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
    14101 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    14161 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    14221 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    14281 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    14341 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    14401 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    14461 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    14521 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    14581 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    14641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    14701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    14761 tttccccctg gaagctccct cgtgcgctct cctgtt  
//
