LOCUS       CaTCH_BC1               8101 bp    DNA        circular     26-MAY-2020
DEFINITION  .
ACCESSION   urn.local...1c8-5frjtwu
VERSION     urn.local...1c8-5frjtwu
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1678..1745
                     /locus_tag="BC1"
                     /label="BC1"
                     /ApEinfo_label="BC1"
                     /ApEinfo_fwdcolor="#fdcc28"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             105..287
                     /Description="3' end of full length 3'LTR"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG-Cas9(PX335)-partial U6;
                     SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Truncated 3'LTR"
                     /label="Truncated 3'LTR"
                     /ApEinfo_label="Truncated 3'LTR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1902..2621
                     /locus_tag="GFP"
                     /label="GFP"
                     /ApEinfo_label="GFP"
                     /ApEinfo_fwdcolor="#009c0d"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    105..287
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5'LTR"
                     /label="5'LTR"
                     /ApEinfo_label="5'LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    106..201
                     /gene="R"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; SGEP-GFP-tTA-Linker; VPR
                     sffv; pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-PX335-P2A-GFP Delta BbsI"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="R"
                     /label="R"
                     /ApEinfo_label="R"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1896..1901
                     /locus_tag="Kozak"
                     /label="Kozak"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#9becff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1761..1884
                     /vntifkey=29
                     /locus_tag="From TRE3G (contains mCMV)"
                     /label="From TRE3G (contains mCMV)"
                     /ApEinfo_label="From TRE3G (contains mCMV)"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1409..1442
                     /locus_tag="SBS-12 Seq - Fwd"
                     /label="SBS-12 Seq - Fwd"
                     /ApEinfo_label="SBS-12 Seq - Fwd"
                     /ApEinfo_fwdcolor="#ff8200"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    204..285
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     lentiCas9-Blast (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U5"
                     /label="U5"
                     /ApEinfo_label="U5"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    332..457
                     /note="HIV-1 Psi; packaging signal of human
                     immunodeficiency virus  type 1"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1 Psi"
                     /label="HIV-1 Psi"
                     /ApEinfo_label="HIV-1 Psi"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1414..1436
                     /locus_tag="1st PCR - Primer-binding site"
                     /label="1st PCR - Primer-binding site"
                     /ApEinfo_label="1st PCR - Primer-binding site"
                     /ApEinfo_fwdcolor="#c3112f"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1414..1432
                     /locus_tag="2nd-primer binding site"
                     /label="2nd-primer binding site"
                     /ApEinfo_label="2nd-primer binding site"
                     /ApEinfo_fwdcolor="#8f0a20"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    348..463
                     /gene="HIV-1 pack"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LUPG FKBP tTA P2A;
                     RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1 pack"
                     /label="HIV-1 pack"
                     /ApEinfo_label="HIV-1 pack"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            396..440
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.TETO.SFFV.G (modified); pRRL.TETOrev.SFF;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_psi_pack"
                     /label="HIV-1_psi_pack"
                     /ApEinfo_label="HIV-1_psi_pack"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    396..440
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR-improved tracer; pLV-tTRKRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="RIKPB, pLV-tTRKRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6;
                     LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIEPH;
                     RIEPTETronly; RIKPB; RIKPB Copy; RIKPH;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     lentiCas9-Blast (modified); lentiCrispr mCherryP2AneoR;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLV-tTRKRAB (modified); pLVTHM (modified);
                     pLentiCRISPR-P2A-GFP vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pLentisgRNA Thy1.1-P2A-neo +Kosak; pLentisgRNA
                     Thy1.1-P2A-neo +KosakDeltaBbsI Library Vector;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_psi_pack(1)"
                     /label="HIV-1_psi_pack(1)"
                     /ApEinfo_label="HIV-1_psi_pack"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    441..794
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LUPG FKBP tTA P2A;
                     RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Gag"
                     /label="Gag"
                     /ApEinfo_label="Gag"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    441..484
                     /gene="dGag'"
                     /SECDrawAs="Region"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.PPT.CBX3.SF; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="dGag' misc"
                     /label="dGag' misc"
                     /ApEinfo_label="dGag' misc"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    485..805
                     /gene="'dGag"
                     /SECDrawAs="Region"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.PPT.CBX3.SF; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="'dGag misc"
                     /label="'dGag misc"
                     /ApEinfo_label="'dGag misc"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    855..870
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_int"
                     /label="SV40_int"
                     /ApEinfo_label="SV40_int"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    950..1183
                     /X-Transferred_Translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRR
                     VVQREKRAVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQ
                     QHLLQLTVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLE
                     QIWNHTTWMEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNW
                     LWYIKLFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEE
                     GGERDRDRSIRLVNGSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALK
                     YWWNLLQYWSQELKNSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLE
                     RILL*;
                     MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGA
                     ASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLKDQ
                     QLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDREINNYTSLIHSLIEE
                     SQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFAVLSIVN
                     RVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSRRYRCRR*;
                     MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGA
                     ASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLKDQ
                     QLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDREINNYTSLIHSLIEE
                     SQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFAVLSIVN
                     RVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSRRYRDVDDKLCK
                     DG*"
                     /note="RRE; The Rev response element (RRE) of HIV-1 allows
                     for Rev-dependent mRNA export from the nucleus to
                     thecytoplasm."
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Exported; LUPG FKBP tTA P2A;
                     LUPG-Cas9(PX335)-partial U6; LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR; pCMVR8.74 D64V;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLV-tTRKRAB (modified);
                     pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +KosakDeltaBbsI Library
                     Vector; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RRE"
                     /label="RRE"
                     /ApEinfo_label="RRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    961..1165
                     /Description="Rev responsive element; Unknown feature
                     type:Regulatory_Seq"
                     /gene="RRE(HIV) reg"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLVTHM
                     (modified); pLentiCRISPR-P2A-GFP vol.2; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RRE; RRE(HIV) reg"
                     /label="RRE; RRE(HIV) reg"
                     /ApEinfo_label="RRE; RRE(HIV) reg"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1678..1697
                     /locus_tag="Site-C"
                     /label="Site-C"
                     /ApEinfo_label="Site-C"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2640..2662
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; lentiCas9-Blast (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-GFP vol.2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HRP"
                     /label="HRP"
                     /ApEinfo_label="HRP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1726..1745
                     /locus_tag="Site-A"
                     /label="Site-A"
                     /ApEinfo_label="Site-A"
                     /ApEinfo_fwdcolor="#51e4ec"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2664..2787
                     /Description="central polypurine tract (cPPT) and the
                     central termination sequence (CTS)"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LUPG-Cas9(PX335)-partial U6;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-GFP vol.2; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="cPPT; cPPT; cPPT/CTS"
                     /label="cPPT; cPPT; cPPT/CTS"
                     /ApEinfo_label="cPPT; cPPT; cPPT/CTS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1702..1721
                     /locus_tag="Site-B"
                     /label="Site-B"
                     /ApEinfo_label="Site-B"
                     /ApEinfo_fwdcolor="#b8d8f2"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2824..3074
                     /X-Transferred_From="Clipboard Contents 2; lentiCrispr
                     mCherryP2AneoR; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-PX335-P2A-GFP Delta BbsI"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter uukaryotic"
                     /locus_tag="EF1as"
                     /label="EF1as"
                     /ApEinfo_label="EF1as"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             3097..3816
                     /X-Transferred_From="TRE3G-dsRed-miRE (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="TRE3G-dsRed-miRE (modified),
                     pLKO.sgRNA.2stepGibson Reporter"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="eBFP2"
                     /label="eBFP2"
                     /ApEinfo_label="eBFP2"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3554..3564)
                     /Recognition_pattern="GACNNNNRTGA(12/10)"
                     /annotation_group="GACNNNNRTGA"
                     /X-Transferred_From="gBlock sgRNA test;
                     pLKO.sgRNA.reporter vector"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="gBlock sgRNA test,
                     pLKO.sgRNA.2stepGibson Reporter, pLKO.sgRNA.reporter
                     vector"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="SdeOSI"
                     /label="SdeOSI"
                     /ApEinfo_label="SdeOSI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3583..3593
                     /Recognition_pattern="AAGNNNNNCTT(13/-24)"
                     /annotation_group="AAGNNNNNCTT"
                     /X-Transferred_From="LMN.miRE.Pten.1523;
                     TPGPB-Cas9lentiCRISPR); TRE3G-Cas9-PGK-Blast; gBlock sgRNA
                     test; pLKO.sgRNA.reporter vector"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="gBlock sgRNA test,
                     pLKO.sgRNA.2stepGibson Reporter, pLKO.sgRNA.reporter
                     vector"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="FalI"
                     /label="FalI"
                     /ApEinfo_label="FalI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3603..3613
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /X-Transferred_From="gBlock sgRNA test;
                     pLKO.sgRNA.reporter vector"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="gBlock sgRNA test,
                     pLKO.sgRNA.2stepGibson Reporter, pLKO.sgRNA.reporter
                     vector"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="PflMI"
                     /label="PflMI"
                     /ApEinfo_label="PflMI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3833..4408
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pLentiCRISPR-P2A-GFP vol.2; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             complement(4302..4313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /X-Transferred_Translation="IEGR"
                     /X-Transferred_From="Clipboard Contents 6; Exported;
                     SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Factor Xa recognition and cleavage site CDS"
                     /label="Factor Xa recognition and cleavage site CDS"
                     /ApEinfo_label="Factor Xa recognition and cleavage site
                     CDS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     4474..4495
                     /SECDrawAs="Label"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; SGEP-GFP-tTA-Linker; VPR
                     sffv; pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U3PPT"
                     /label="U3PPT"
                     /ApEinfo_label="U3PPT"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             4490..4725
                     /X-Transferred_From="pLentiCRISPR;
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="RIKPB"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A; RIEPH; RIEPTETronly; RIKPB;
                     RIKPB Copy; RIKPH; SGEP-GFP-tTA-Linker; VPR sffv; backup
                     of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pLentiCRISPR; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="3' LTR SIN"
                     /label="3' LTR SIN"
                     /ApEinfo_label="3' LTR SIN"
                     /ApEinfo_fwdcolor="#8882be"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4490..4543
                     /invitrogen=1410000
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="delta U3"
                     /label="delta U3"
                     /ApEinfo_label="delta U3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            4491..4543
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.TETO.SFFV.G (modified); pRRL.TETOrev.SFF;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="delta_U3"
                     /label="delta_U3"
                     /ApEinfo_label="delta_U3"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4491..4724
                     /gene="SIN"
                     /SECDrawAs="Region"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.PPT.CBX3.SF; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SIN misc"
                     /label="SIN misc"
                     /ApEinfo_label="SIN misc"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             4544..4724
                     /note="5' LTR (truncated); truncated 5' long terminal
                     repeat (LTR) from HIV-1"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5' LTR (truncated)"
                     /label="5' LTR (truncated)"
                     /ApEinfo_label="5' LTR (truncated)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4544..4726
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5'LTR(1)"
                     /label="5'LTR(1)"
                     /ApEinfo_label="5'LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4544..4725
                     /gene="RU5"
                     /SECDrawAs="Region"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pRRL.PPT.CBX3.SF; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RU5 misc"
                     /label="RU5 misc"
                     /ApEinfo_label="RU5 misc"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4545..4640
                     /gene="R"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; SGEP-GFP-tTA-Linker; VPR
                     sffv; pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-PX335-P2A-GFP Delta BbsI"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="R(1)"
                     /label="R(1)"
                     /ApEinfo_label="R"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4641..4725
                     /gene="U5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LUPG FKBP tTA P2A;
                     RIEPTETronly; SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U5(1)"
                     /label="U5(1)"
                     /ApEinfo_label="U5"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4802..4933
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 EEL poly(A)s"
                     /label="SV40 EEL poly(A)s"
                     /ApEinfo_label="SV40 EEL poly(A)s"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4893..4912
                     /X-Transferred_Translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDF
                     PSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILF
                     FLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFK
                     KPPTPPPEPET*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR-improved tracer; pLV-tTRKRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_Translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPS
                     ELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFL
                     TPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKP
                     PTPPPEPET*"
                     /Transferred_From="RIKPB, pLV-tTRKRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_Translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIG
                     CSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAG
                     RDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERART
                     RMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFS
                     GFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDE
                     FF*;
                     MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFCLLPDGLIPVLIVSR
                     KHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGRPFELFIVAGQIQEI
                     NVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFDLFISFRLEKVDGVF
                     FEA*;
                     MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKE
                     KAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICK
                     GVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; M-ST1-VPR
                     (modified); RIEPH; RIEPTETronly; RIKPB; RIKPB Copy; RIKPH;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCrispr
                     mCherryP2AneoR; pAdSh.PGK.Cas9 (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLV-tTRKRAB (modified); pLVTHM (modified);
                     pLentiCRISPR-P2A-GFP vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pLentisgRNA Thy1.1-P2A-neo +Kosak; pLentisgRNA
                     Thy1.1-P2A-neo +KosakDeltaBbsI Library Vector;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="EBV_rev_primer"
                     /label="EBV_rev_primer"
                     /ApEinfo_label="EBV_rev_primer"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4943..5006
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LUPG FKBP tTA P2A;
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9GFPERT2;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 3x21bp repeat"
                     /label="SV40 3x21bp repeat"
                     /ApEinfo_label="SV40 3x21bp repeat"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      4963..5098
                     /note="SV40 ori; SV40 origin of replication"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 ori rep origin"
                     /label="SV40 ori rep origin"
                     /ApEinfo_label="SV40 ori rep origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5043..5055
                     /Recognition_pattern="GGCCNNNN^NGGCC"
                     /annotation_group="GGCCNNNNNGGCC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; Puro-P2A-tagBFP
                     reporter; RIEPTETronly; RIKPB; RIKPB Copy; RIKPH;
                     SGEP-GFP-tTA-Linker; TPGPB-Cas9(PX330);
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; pCMVR8.74
                     D64V; pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL.SFFV-rtTA3-IRES-EcoR-PGK-Puro (pRRL.RIEP) Copy;
                     pRRL.TETO.SFFV.G (modified); pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="SfiI"
                     /label="SfiI"
                     /ApEinfo_label="SfiI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5322..5628
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGASLIKHW*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR-improved tracer; pGEM-T vector;
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="RIKPB"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6;
                     LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIEPH;
                     RIEPTETronly; RIKPB; RIKPB Copy; RIKPH;
                     SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo; lentiCrispr
                     mCherryP2AneoR; pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pLentiCRISPR-P2A-GFP vol.2; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="f1_origin"
                     /label="f1_origin"
                     /ApEinfo_label="f1_origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5576..5587
                     /Recognition_pattern="GACNNNN^NNGTC"
                     /annotation_group="GACNNNNNNGTC"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A;
                     LentiCrisprmCherryP2Apuro-improved tracer; RIEPTETronly;
                     RIKPB; RIKPB Copy; RIKPH; SGEP-GFP-tTA-Linker; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="DrdI, AasI, DseDI"
                     /label="DrdI, AasI, DseDI"
                     /ApEinfo_label="DrdI, AasI, DseDI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5755..5766
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10);
                     CAANNNNNGTGG(12/10)"
                     /annotation_group="CAANNNNNGTGG"
                     /X-Transferred_From="Clipboard Contents 2; LUPG FKBP tTA
                     P2A; LentiCrisprmCherryP2Apuro-improved tracer;
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="CspCI"
                     /label="CspCI"
                     /ApEinfo_label="CspCI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5778..5797)
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Pinco14"
                     /label="Pinco14"
                     /ApEinfo_label="Pinco14"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5792..5896
                     /invitrogen=2330000
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LMHT-MYCTETR; LMHT-MYCTETRSNLS; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="bla (Amp) promoter; bla promoter"
                     /label="bla (Amp) promoter; bla promoter"
                     /ApEinfo_label="bla (Amp) promoter; bla promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6599..6609)
                     /Recognition_pattern="GAYNNNNNVTC(13/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="Hin4I"
                     /label="Hin4I"
                     /ApEinfo_label="Hin4I"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6603..6622)
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents; Clipboard
                     Contents 2; Clipboard Contents 6; ES-FUCCI (modified);
                     LMHT-MYCTETR; LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A;
                     RIEPTETronly; SGEP-GFP-tTA-Linker;
                     TRE3G-Cas9+LinkerERT2-P2A-GFP; TRE3G-Cas9ERT2-P2A-GFP;
                     TRE3G-Cas9GFPERT2; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     lentiCas9-Blast (modified); pCR4-TOPO (modified);
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLVTHM (modified); pLentiCRISPR-P2A-GFP vol.2;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-CBX3-sffv-GFP-mirE-PGKneo Copy;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL-UCOeminimal-4xTET-sffv-GFP-mirE-PGKneo;
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="Pinco13"
                     /label="Pinco13"
                     /ApEinfo_label="Pinco13"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6673..6683)
                     /Recognition_pattern="GAYNNNNNVTC(13/8)"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="Hin4I(1)"
                     /label="Hin4I(1)"
                     /ApEinfo_label="Hin4I"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6880..6891)
                     /Recognition_pattern="GACNNNNNNTGG(12/7)"
                     /annotation_group="GACNNNNNNTGG"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; ES-FUCCI (modified); LMHT-MYCTETR;
                     LMHT-MYCTETRSNLS; LUPG FKBP tTA P2A; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; TPGPB-Cas9lentiCRISPR);
                     TPmCPB-Cas9(lentiCRISPR); TRE3G-Cas9+LinkerERT2-P2A-GFP;
                     TRE3G-Cas9ERT2-P2A-GFP; TRE3G-Cas9GFPERT2; VPR sffv;
                     backup of pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pCR4-TOPO (modified); pCRISPR-6xTET-sffv-CAS9-P2A-GFP;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR; pSIN-reporter with
                     desttagBFP; pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="Bsp24I"
                     /label="Bsp24I"
                     /ApEinfo_label="Bsp24I"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6896..7569
                     /Description="3 extra bp in the middle, compared to pLVX
                     ori - ACAAAAAAACC(ACC)GCTACCAG"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LUPG-Cas9(PX335)-partial U6;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-GFP
                     vol.2; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      6906..7525
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGASLIKHW*"
                     /X-Transferred_From="Clipboard Contents; Clipboard
                     Contents 2; LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR; pCMVR8.74 D64V;
                     pCRISPR-6xTET-sffv-CAS9-P2A-GFP; pLentiCRISPR-P2A-Thy1.1;
                     tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="pBR322_origin"
                     /label="pBR322_origin"
                     /ApEinfo_label="pBR322_origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      7343..7563
                     /Description="putative"
                     /X-Transferred_From="AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-
                     U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-
                     ITR (AAV-KPL) (modified); Clipboard Contents 2; Clipboard
                     Contents 6; VPR sffv; pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="PMB1"
                     /label="PMB1"
                     /ApEinfo_label="PMB1"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7708..7718
                     /Recognition_pattern="ACNNNNNCTCC(10/7)"
                     /annotation_group="ACNNNNNCTCC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LMHT-MYCTETR; LMHT-MYCTETRSNLS; RIEPTETronly;
                     SGEP-GFP-tTA-Linker; VPR sffv; pCR4-TOPO (modified);
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-SFFV-TETR-KRAB-PGK-hygroR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="BsaXI"
                     /label="BsaXI"
                     /ApEinfo_label="BsaXI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    7798..7819
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP.; CAP binding site"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; Exported; SGEP-GFP-tTA-Linker; VPR sffv;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="CAP binding site protein bind"
                     /label="CAP binding site protein bind"
                     /ApEinfo_label="CAP binding site protein bind"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(7975..8101,1..286)
                     /X-Transferred_From="Clipboard Contents 2;
                     LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCrispr
                     mCherryP2AneoR; pLentiCRISPR-P2A-Thy1.1; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak; pLentisgRNA Thy1.1-P2A-neo
                     +KosakDeltaBbsI Library Vector; tta-mcherry tag"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5'LTR(2)"
                     /label="5'LTR(2)"
                     /ApEinfo_label="5'LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        join(7977..8101,1..104)
                     /note="RSV promoter; Rous sarcoma virus enhancer/promoter"
                     /X-Transferred_From="Exported"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RSV promoter"
                     /label="RSV promoter"
                     /ApEinfo_label="RSV promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(8079..8090)
                     /Recognition_pattern="CACNNNNNNTCC(12/7)"
                     /annotation_group="CACNNNNNNTCC"
                     /X-Transferred_From="Clipboard Contents 2; Clipboard
                     Contents 6; LUPG FKBP tTA P2A; RIEPTETronly; RIKPB; RIKPB
                     Copy; RIKPH; SGEP-GFP-tTA-Linker; VPR sffv; backup of
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pLentiCRISPR-P2A-Thy1.1;
                     pRRL-4xTEToSFFV-TETr-GFP-KRAB-mirE-PGK-neo;
                     pRRL-4xtetsffv-GFP-mirE-EfIas-KRABP2Aneo;
                     pRRL-CBX3-SFFV-TETr-KRAB-P2A-Hygro;
                     pRRL-EfIa-TETr-KRAB-PGK-hygro Copy; pRRL-PX335-P2A-GFP
                     Delta BbsI; pRRL-SFFV-TETR-KRAB-PGK-hygroR;
                     pRRL.TETO.SFFV.G (modified);
                     pVLH-CBX3-SFFV-TETR-KRAB-P2A-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: restriction site"
                     /locus_tag="TstI"
                     /label="TstI"
                     /ApEinfo_label="TstI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1385..1408
                     /locus_tag="P7-Fwd"
                     /label="P7-Fwd"
                     /ApEinfo_label="P7-Fwd"
                     /ApEinfo_fwdcolor="#fffc00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1746..1765
                     /locus_tag="1st-PCR reverse-primer"
                     /label="1st-PCR reverse-primer"
                     /ApEinfo_label="1st-PCR reverse-primer"
                     /ApEinfo_fwdcolor="#ff8200"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1746..1748
                     /locus_tag="PAM"
                     /label="PAM"
                     /ApEinfo_label="PAM"
                     /ApEinfo_fwdcolor="#d204ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1722..1724
                     /locus_tag="PAM(1)"
                     /label="PAM(1)"
                     /ApEinfo_label="PAM"
                     /ApEinfo_fwdcolor="#d104fe"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1698..1700
                     /locus_tag="PAM(2)"
                     /label="PAM(2)"
                     /ApEinfo_label="PAM"
                     /ApEinfo_fwdcolor="#d104fe"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
       61 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
      121 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      181 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      241 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      301 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      361 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      421 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      481 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      541 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      601 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      661 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
      721 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
      781 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
      841 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
      901 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
      961 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1021 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1081 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1141 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1201 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1261 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1321 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1381 aattCAAGCA GAAGACGGCA TACGAGATGT GACTGGAGTT CAGACGTGTG CTCTTCCGAT
     1441 CTattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1501 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1561 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1621 cacctcccaa ccccgagggg accctatcat atgctgggta ccctgtctag agcGAACGGG
     1681 CGAGCGCGCG CAAATGGcgg cGACTAGTGA TATCCGTCTC TcggcGCTGT GCTCGTTCCA
     1741 CACACcggca ggagaagctt taggcgtgta cggtgggcgc ctataaaagc agagctcgtt
     1801 tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat accaactttc
     1861 cgtaccactt cctaccctcg taaactggag gatccgccac catggtgagc aagggcgagg
     1921 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1981 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2041 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2101 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2161 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2221 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2281 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2341 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2401 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2461 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2521 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2581 ccgccgggat cactctcggc atggacgagc tgtacaagta agaattctag atcttgagac
     2641 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2701 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2761 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     2821 ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2881 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     2941 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     3001 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     3061 gtgtcgtgac gcggatcgta ccccgaattt atgatcatgg tgagcaaggg cgaggagctg
     3121 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3181 agcgtgaggg gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
     3241 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagccacggc
     3301 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3361 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg cacctacaag
     3421 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3481 gtcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc
     3541 cacaacatct atatcatggc cgtcaagcag aagaacggca tcaaggtgaa cttcaagatc
     3601 cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3661 atcggcgacg gccccgtgct gctgcccgac agccactacc tgagcaccca gtccgtgctg
     3721 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttccg caccgccgcc
     3781 gggatcactc tcggcatgga cgagctgtac aagtaacgcg ttaagtcgac aatcaacctc
     3841 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3901 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3961 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4021 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4081 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4141 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4201 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4261 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4321 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4381 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     4441 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4501 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     4561 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4621 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4681 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     4741 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     4801 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4921 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     4981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5041 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5101 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     5161 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5221 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5281 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5341 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5401 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5461 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5521 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5581 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5641 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5701 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5761 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5821 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5881 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5941 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6001 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6061 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6121 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6181 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6241 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6301 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6361 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6421 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6481 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6541 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6601 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6661 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6721 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6781 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6841 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6901 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6961 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7021 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7081 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7141 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7201 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7261 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7321 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7381 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7441 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7501 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7561 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7621 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7681 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7741 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     7801 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     7861 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     7921 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     7981 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     8041 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     8101 g     
//
